Colistin A and colistin B among inhibitory substances of Paenibacillus polymyxa JB05-01-1

Naghmouchi, Karim; Hammami, Riadh; Fliss, Ismaı̈l; Teather, R. M.; Baah, J.; Drider, Djamel

doi:10.1007/s00203-011-0764-z

Cited by 18 publications

(8 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous reports have shown that the anti-fungal mechanism of action of Paenibacillus sp. involves production of potent antifungal compounds including polymyxins, fusaricidins, colistins, volatiles, and lytic enzymes (He et al, 2007 ; Raza et al, 2008 , 2009 , 2015 ; Naghmouchi et al, 2012 ). In particular, Paenibacillus is well-known to combat F. graminearum by employing fusaricidin, a compound named after Fusarium as noted earlier (Kajimura and Kaneda, 1996 , 1997 ; Beatty and Jensen, 2002 ; Choi et al, 2008 ).…”

Section: Discussionmentioning

confidence: 99%

Bacterial endophytes from wild maize suppress Fusarium graminearum in modern maize and inhibit mycotoxin accumulation

et al. 2015

View full text Add to dashboard Cite

Wild maize (teosinte) has been reported to be less susceptible to pests than their modern maize (corn) relatives. Endophytes, defined as microbes that inhabit plants without causing disease, are known for their ability to antagonize plant pests and pathogens. We hypothesized that the wild relatives of modern maize may host endophytes that combat pathogens. Fusarium graminearum is the fungus that causes Gibberella Ear Rot (GER) in modern maize and produces the mycotoxin, deoxynivalenol (DON). In this study, 215 bacterial endophytes, previously isolated from diverse maize genotypes including wild teosintes, traditional landraces and modern varieties, were tested for their ability to antagonize F. graminearum in vitro. Candidate endophytes were then tested for their ability to suppress GER in modern maize in independent greenhouse trials. The results revealed that three candidate endophytes derived from wild teosintes were most potent in suppressing F. graminearum in vitro and GER in a modern maize hybrid. These wild teosinte endophytes could suppress a broad spectrum of fungal pathogens of modern crops in vitro. The teosinte endophytes also suppressed DON mycotoxin during storage to below acceptable safety threshold levels. A fourth, less robust anti-fungal strain was isolated from a modern maize hybrid. Three of the anti-fungal endophytes were predicted to be Paenibacillus polymyxa, along with one strain of Citrobacter. Microscopy studies suggested a fungicidal mode of action by all four strains. Molecular and biochemical studies showed that the P. polymyxa strains produced the previously characterized anti-Fusarium compound, fusaricidin. Our results suggest that the wild relatives of modern crops may serve as a valuable reservoir for endophytes in the ongoing fight against serious threats to modern agriculture. We discuss the possible impact of crop evolution and domestication on endophytes in the context of plant defense.

show abstract

Section: Discussionmentioning

confidence: 99%

Bacterial endophytes from wild maize suppress Fusarium graminearum in modern maize and inhibit mycotoxin accumulation

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The main roles of P. polymyxa are to stimulate plant growth through the production of various plant hormones and to promote immunity of the rhizosphere . In addition, P. polymyxa strains are also known to produce several antibiotics and hydrolytic enzymes, including polymyxins, fusaricidins, colistin, proteases, β ‐1,3‐glucanases, cellulases, xylanase, chitinases, and so on, which play important roles in the biocontrol of plant pathogens . P. polymyxa E681 was isolated from the rhizosphere of winter barley grown in South Korea .…”

Section: Introductionmentioning

confidence: 99%

Proteomic analyses of the interaction between the plant‐growth promoting rhizobacterium Paenibacillus polymyxa E681 and Arabidopsis thaliana

et al. 2016

View full text Add to dashboard Cite

Plant growth-promoting rhizobacteria (PGPR) facilitate the plant growth and enhance their induced systemic resistance (ISR) against a variety of environmental stresses. In this study, we carried out integrative analyses on the proteome, transcriptome, and metabolome to investigate Arabidopsis root and shoot responses to the well-known PGPR strain Paenibacillus polymyxa (P. polymyxa) E681. Shoot fresh and root dry weights were increased, whereas root length was decreased by treatment with P. polymyxa E681. 2DE approach in conjunction with MALDI-TOF/TOF analysis revealed a total of 41 (17 spots in root, 24 spots in shoot) that were differentially expressed in response to P. polymyxa E681. Biological process- and molecular function-based bioinformatics analysis resulted in their classification into seven different protein groups. Of these, 36 proteins including amino acid metabolism, antioxidant, defense and stress response, photosynthesis, and plant hormone-related proteins were up-regulated, whereas five proteins including three carbohydrate metabolism- and one amino acid metabolism-related, and one unknown protein were down-regulated, respectively. A good correlation was observed between protein and transcript abundances for the 12 differentially expressed proteins during interactions as determined by qPCR analysis. Metabolite analysis using LC-MS/MS revealed highly increased levels of tryptophan, indole-3-acetonitrile (IAN), indole-3-acetic acid (IAA), and camalexin in the treated plants. Arabidopsis plant inoculated P. polymyxa E681 also showed resistance to Botrytis cinerea infection. Taken together these results suggest that P. polymyxa E681 may promote plant growth by induced metabolism and activation of defense-related proteins against fungal pathogen.

show abstract

“…Auxin, cytokinin, indole‐3‐acetic acid (IAA) and other indolic compounds referring to simulating plant growth were found in P. polymyxa culture (Lebuhn et al , ; Timmusk et al , ; Phi et al , ). P. polymyxa strains are also known to produce several antibiotics and hydrolytic enzymes, including polymyxins, fusaricidins, colistin, proteases, β ‐1,3‐glucanases, cellulases, xylanase, chitinases and so on, which play important roles in the biocontrol of plant pathogens (Parry et al , ; Beatty & Jensen, ; He et al , ; Li & Jensen, ; Raza et al , ; Ariza et al , ; Naghmouchi et al , ). Some strains of P. polymyxa were capable to colonise host root tips and form robust biofilms (Timmusk et al , ) as well as change plant defence‐related gene expression (Timmusk & Wagner, ).…”

Section: Introductionmentioning

confidence: 99%

Induced systemic resistance and growth promotion in tomato by an indole‐3‐acetic acid‐producing strain of Paenibacillus polymyxa

Liang

Zhang³

et al. 2014

Annals of Applied Biology

View full text Add to dashboard Cite

Paenibacillus polymyxa strain CF05, a plant-growth-promoting rhizobacterium (PGPR) showed inhibitory effect on Fusarium oxysporum f. sp. lycopersici in vitro, causal agent of Fusarium-wilt of tomato. In the greenhouse experiments, tomatoes inoculated with strain CF05 promoted the significant growth. Meanwhile, strain CF05 suppressed the Fusarium-wilt with 78.24% in control efficiency, which was similar to the carbendazim treatment. The bacterial culture supernatant was found to have the presence of indole-3-acetic acid (IAA), which could be accumulated throughout the growth. Furthermore, strain CF05 could greatly stimulate the activities of phenylalanine ammonialyase (PAL), polyphenol oxidase (PPO), peroxidase (POD) and superoxide dismutase (SOD) in either the absence or the presence of fungal pathogen, and the stimulation stayed throughout the entire experimental assessment. Activities of POD and SOD reached the maximum amount at the second day post-inoculation (dpi) after inoculation while activities of PAL and PPO reached the maximum amount at the third dpi and the fourth dpi, respectively. Moreover, strain CF05 could stimulate the H 2 O 2 and phenol contents in the initial phase after pathogen inoculation. These results suggest that the sustained and timely induction of defence enzymes and accumulation of H 2 O 2 and phenolics treated with P. polymyxa might have contributed to restriction of invasion of fungal pathogen in tomato roots. In summary, P. polymyxa strain CF05, which elicited both plant growth promotion and induction of defence response, could be potential agent to improve the yield of tomato and manage Fusarium-wilt disease.

show abstract

Colistin A and colistin B among inhibitory substances of Paenibacillus polymyxa JB05-01-1

Cited by 18 publications

References 26 publications

Bacterial endophytes from wild maize suppress Fusarium graminearum in modern maize and inhibit mycotoxin accumulation

Bacterial endophytes from wild maize suppress Fusarium graminearum in modern maize and inhibit mycotoxin accumulation

Proteomic analyses of the interaction between the plant‐growth promoting rhizobacterium Paenibacillus polymyxa E681 and Arabidopsis thaliana

Induced systemic resistance and growth promotion in tomato by an indole‐3‐acetic acid‐producing strain of Paenibacillus polymyxa

Contact Info

Product

Resources

About