Colistin resistance in<i>Salmonella</i>and<i>Escherichia coli</i>isolates from a pig farm in Great Britain

Anjum, Muna F.; Duggett, Nicholas; AbuOun, Manal; Randall, Luke; Nuñéz-García, Javier; Ellis, Richard J.; Rogers, Jon; Horton, Robert A.; Brena, Camilla; Williamson, Susanna; Martelli, Francesca; Davies, Robert; Teale, Christopher

doi:10.1093/jac/dkw149

Cited by 128 publications

(111 citation statements)

References 27 publications

Supporting

Mentioning

101

Contrasting

Unclassified

Order By: Relevance

“…The complete sequence of a ϳ60-kb conjugative plasmid, recovered from the S. Enteritidis HSSH23 strain, pHSSH23-MCR1, was obtained. It was found to belong to an IncI2-type plasmid with a backbone structure similar to that of pHNSHP45 (GenBank accession number KP347127) and other plasmids reported previously (8,9). Compared to plasmid pHNSHP45, pHSSH23-MCR1 with a size of 60,035 bp was found to lack an IS683-mediated fragment (Table 2 and Fig.…”

Section: Resultssupporting

confidence: 62%

“…Java, and 1 S. enterica serotype Virchow strain (8). The mcr-1 gene was subsequently reported to be recoverable from Salmonella strains isolated from food, animals, and clinical specimens in Europe, the United States, and China (9)(10)(11)(12)(13)(14) and was found to be harbored mainly by IncX4 and IncI1 plasmids of various sizes (10,11). However, these data remain scattered and do not provide a comprehensive view on either the prevalence of the mcr-1 gene in Salmonella or the transmission kinetics of the different types of mobile elements that harbor such resistance determinants.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Prevalence and Molecular Characterization of mcr-1 -Positive Salmonella Strains Recovered from Clinical Specimens in China

Cui

Zhang

Gu³

et al. 2017

Antimicrob Agents Chemother

View full text Add to dashboard Cite

The recently discovered colistin resistance element, mcr-1, adds to the list of antimicrobial resistance genes that rapidly erode the antimicrobial efficacy of not only the commonly used antibiotics but also the last-line agents of carbapenems and colistin. This study investigated the prevalence of the mobile colistin resistance determinant mcr-1 in Salmonella strains recovered from clinical settings in China and the transmission potential of mcr-1-bearing mobile elements harbored by such isolates. The mcr-1 gene was recoverable in 1.4% of clinical isolates tested, with the majority of them belonging to Salmonella enterica serotype Typhimurium. These isolates exhibited diverse pulsed-field gel electrophoresis (PFGE) profiles and high resistance to antibiotics other than colistin and particularly to cephalosporins. Plasmid analysis showed that mcr-1 was carried on a variety of plasmids with sizes ranging from ϳ30 to ϳ250 kb, among which there were conjugative plasmids of ϳ30 kb, ϳ60 kb, and ϳ250 kb and nonconjugative plasmids of ϳ140 kb, ϳ180 kb, and ϳ240 kb. Sequencing of representative mcr-1-carrying plasmids revealed that all conjugative plasmids belonged to the IncX4, IncI2, and IncHI2 types and were highly similar to the corresponding types of plasmids reported previously. Nonconjugative plasmids all belonged to the IncHI2 type, and the nontransferability of these plasmids was attributed to the loss of a region carrying partial or complete tra genes. Our data revealed that, similar to the situation in Escherichia coli, mcr-1 transmission in Salmonella was accelerated by various plasmids, suggesting that transmission of mcr-1-carrying plasmids between different species of Enterobacteriaceae may be a common event.

show abstract

Section: Resultssupporting

confidence: 62%

mentioning

confidence: 99%

Prevalence and Molecular Characterization of mcr-1 -Positive Salmonella Strains Recovered from Clinical Specimens in China

Cui

Zhang

Gu³

et al. 2017

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…Isolates from Belgium and APHA were sequenced at APHA as described by Anjum et al (2016). Isolates from PHE and AFBI were sequenced at PHE Genomic Service Unit on Illumina HiSeq platform using Nextera XT library preparations from DNA extracted on QIAsymphony instrument—DNA DSP mini kit.…”

Section: Methodsmentioning

confidence: 99%

Livestock-Associated Methicillin Resistant Staphylococcus aureus (LA-MRSA) Clonal Complex (CC) 398 Isolated from UK Animals belong to European Lineages

et al. 2016

Self Cite

View full text Add to dashboard Cite

In recent years, there has been an increase in the number of livestock-associated methicillin resistant Staphylococcus aureus (LA-MRSA) clonal complex (CC) 398 recovered from S. aureus isolated animals in the UK. To determine possible origins of 12 LA-MRSA CC398 isolates collected after screening more than a thousand S. aureus animal isolates from the UK between 2013 and 2015, whole genome sequences (WGS) of CC398 European, including UK, and non-European isolates from diverse animal hosts were compared. Phylogenetic reconstruction applied to WGS data to assess genetic relatedness of all 89 isolates, clustered the 12 UK CC398 LA-MRSA within the European sub-lineages, although on different nodes; implicating multiple independent incursions into the UK, as opposed to a single introduction followed by clonal expansion. Three UK isolates from healthy pigs and one from turkey clustered within the cassette chromosome recombinases ccr C S. aureus protein A (spa)-type t011 European sub-lineage and three UK isolates from horses within the ccrA2B2 t011 European sub-lineage. The remaining UK isolates, mostly from pigs, clustered within the t034 European lineage. Presence of virulence, antimicrobial (AMR), heavy metal (HMR), and disinfectant (DR) resistance genes were determined using an in-house pipeline. Most, including UK isolates, harbored resistance genes to ≥3 antimicrobial classes in addition to β-lactams. HMR genes were detected in most European ccrC positive isolates, with >80% harboring czrC, encoding zinc and cadmium resistance; in contrast ~60% ccrC isolates within non-European lineages and 6% ccrA2B2 isolates showed this characteristic. The UK turkey MRSA isolate did not harbor φAVβ avian prophage genes (SAAV_2008 and SAAV_2009) present in US MSSA isolates from turkey and pigs. Absence of some of the major human-associated MRSA toxigenic and virulence genes in the UK LA-MRSA animal isolates was not unexpected. Therefore, we can conclude that the 12 UK LA-MRSA isolates collected in the past 2 years most likely represent separate incursions into the UK from other European countries. The presence of zinc and cadmium resistance in all nine food animal isolates (pig and poultry), which was absent from the 3 horse isolates may suggest heavy metal use/exposure has a possible role in selection of some MRSA.

show abstract

“…To the best of our knowledge, this is the first report of an IncY plasmid carrying the mcr-1 gene. To date, the mcr-1 gene has been found in seven different plasmid incompatibility groups (IncI2 [20], IncFII [21], IncX4 [22], IncHI1 [15], IncHI2 [23], IncP [22], and IncF [10]), suggesting the wide distribution and high dissemination capacity of mcr-1-carrying plasmids. Given the fact that many phage-related genes are encoded by pMCR-1-P3 and most are homologous to the complete E. coli genome (GenBank accession number CP007392), this plasmid was likely formed via homologous recombination from a prophage that was originally located in the E. coli genome.…”

mentioning

confidence: 99%

A Phage-Like IncY Plasmid Carrying the mcr-1 Gene in Escherichia coli from a Pig Farm in China

Zhang

Feng

Liu

et al. 2017

Antimicrob Agents Chemother

View full text Add to dashboard Cite

We report here a new type of plasmid that carries the mcr-1 gene, the pMCR-1-P3 plasmid, harbored in an Escherichia coli strain isolated from a pig farm in China. pMCR-1-P3 belongs to the IncY incompatibility group and is a phage-like plasmid that contains a large portion of phage-related sequences. The backbone of this plasmid is different from that of other mcr-1-carrying plasmids reported previously.

show abstract

Colistin resistance inSalmonellaandEscherichia coliisolates from a pig farm in Great Britain

Cited by 128 publications

References 27 publications

Prevalence and Molecular Characterization of mcr-1 -Positive Salmonella Strains Recovered from Clinical Specimens in China

Prevalence and Molecular Characterization of mcr-1 -Positive Salmonella Strains Recovered from Clinical Specimens in China

Livestock-Associated Methicillin Resistant Staphylococcus aureus (LA-MRSA) Clonal Complex (CC) 398 Isolated from UK Animals belong to European Lineages

A Phage-Like IncY Plasmid Carrying the mcr-1 Gene in Escherichia coli from a Pig Farm in China

Contact Info

Product

Resources

About