Colistin-resistant Pseudomonas aeruginosa clinical strains with defective biofilm formation

Azimi, Leila; Lari, Abdolaziz Rastegar

doi:10.3205/dgkh000328

Cited by 11 publications

(4 citation statements)

References 12 publications

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“…Based on the literature, the burden of Gram-negative resistance bacteria as a major cause of hospital-acquired infection is increasing day by day [ 7 – 9 ]. For years, carbapenem was used as one of the last lines of antibiotic therapy for resistant bacteria, especially ESBL producing [ 10 , 11 ]. Resistance to carbapenem leads to serious challenges for physicians and push them to use colistin as the last option, despite of its nephrotoxic effect.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Susceptibility Patterns for Carbapenem-Resistant Enterobacteriaceae

Armin

Fallah

Karimi

et al. 2023

International Journal of Microbiology

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Carbapenem is a broad-spectrum beta-lactam antibiotic considered the last choice for the treatment of antibiotic-resistant Gram-negative bacteria. Thus, the increasing rate of carbapenem resistance (CR) in Enterobacteriaceae is an urgent public health threat. This study aimed to evaluate the antibiotic susceptibility pattern of carbapenem-resistant Enterobacteriaceae (CRE) to new and old antibiotics. In this study, Klebsiella pneumoniae, E. coli, and Enterobacter spp. were collected from 10 hospitals in Iran for one year. CRE is recognized by resistance to meropenem and/or imipenem disk after identification of the collected bacteria. Antibiotic susceptibility of CRE against fosfomycin, rifampin, metronidazole, tigecycline, and aztreonam was detected by disk diffusion method and colistin by MIC. In this study, 1222 E. coli, 696 K. pneumoniae, and 621 Enterobacter spp. were collected from 10 hospitals in Iran in one year. Fifty-four E. coli (4.4%), 84 K. pneumoniae (12%), and 51 Enterobacter spp. (8.2%) were CRE. All CRE strains were resistant to metronidazole and rifampicin. Tigecycline has the highest sensitivity on CRE and levofloxacin for Enterobacter spp. Tigecycline showed an acceptable effectiveness rate of sensitivity on the CRE strain. Therefore, we suggest that clinicians consider this valuable antibiotic to treat CRE.

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Section: Discussionmentioning

confidence: 99%

Antibiotic Susceptibility Patterns for Carbapenem-Resistant Enterobacteriaceae

Armin

Fallah

Karimi

et al. 2023

International Journal of Microbiology

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“…Moreover, the low prevalence of mcr genes in our population might be partially explained by the fact that colistin-resistance in Enterobacterales has been linked mainly to mgrB mutations [ 35 ], rather than to plasmid-mediated genes. In the case of P. aeruginosa , the main drivers of colistin resistance have also been shown to be mainly due to chromosome mutations leading to the overexpression of efflux pumps or defective biofilm formation [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital

Galindo-Méndez,

Navarrete-Salazar,

Pacheco-Vásquez

et al. 2023

Microorganisms

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The aim of this study was to determine the prevalence of plasmid-mediated colistin resistance mcr-1 to mcr-5 genes among colistin and multi-drug-resistant Gram-negative bacilli strains isolated from patients in a tertiary hospital in Toluca, Mexico. The presence of mcr genes among the 241 strains collected was assessed by PCR. In the case of mcr-carrying E. coli, further PCR tests were performed to determine the presence of blaCTX-M and whether the strains belonged to the O25b-ST131 clone. Conjugation experiments were also carried out to assess the horizontal transmission of colistin resistance. A total of twelve strains (5.0%), of which four were E. coli; four were P. aeruginosa; three were K. pneumoniae, and one E. cloacae, were found to be resistant to colistin. Of these strains, two E. coli isolates were found to carry mcr-1, and Southern blot hybridization demonstrated its presence on an approximately 60 kb plasmid. Both mcr-1-carrying E. coli strains were found to co-express blaCTX-M, belong to the O25b-ST131 clone, and horizontally transmit their colistin resistance. The results of this study confirm the presence of plasmid-mediated colistin resistance in hospitalized patients in Mexico and demonstrated that the multi-drug-resistant O25b-ST131 E. coli clone can acquire mcr genes and transmit such resistance traits to other bacteria.

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“…A number of resistance mechanisms such as target modification, limited permeability of the cell wall, increased activity of efflux pumps and antibiotic hydrolysing enzymes that render antibiotics ineffective have been documented [ 3 , 4 ]. Biofilm formation leads to cross resistance due to low penetration and plays a vital role in the development of preliminary antibiotic resistance mechanisms in A. baumannii and P. aeruginosa [ 5 , 8 ]. The World Health Organization classified carbapenem resistant A. baumannii and carbapenem resistant P. aeruginosa as critical pathogens that require urgent research and development for new drug molecules [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of MMV Pandemic Response Box compounds to identify potent compounds against clinically relevant bacterial and fungal clinical isolates in vitro

Sivasankar,

Boppe,

Grobusch

et al. 2024

New Microbes and New Infections

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Colistin-resistant Pseudomonas aeruginosa clinical strains with defective biofilm formation

Cited by 11 publications

References 12 publications

Antibiotic Susceptibility Patterns for Carbapenem-Resistant Enterobacteriaceae

Antibiotic Susceptibility Patterns for Carbapenem-Resistant Enterobacteriaceae

Detection of Plasmid-Mediated Resistance against Colistin in Multi-Drug-Resistant Gram-Negative Bacilli Isolated from a Tertiary Hospital

Evaluation of MMV Pandemic Response Box compounds to identify potent compounds against clinically relevant bacterial and fungal clinical isolates in vitro

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