Collaborative work on evaluation of ovarian toxicity 11) Two- or four-week repeated-dose studies and fertility study of ethylene glycol monomethyl ether in female rats

Dodo, Tetsushi; Taketa, Yoshikazu; Sugiyama, Mayumi; Inomata, Akira; Sonoda, Jiro; Okuda, Yasuyuki; Mineshima, Hiroshi; Hosokawa, Satoru; Aoki, Toyohiko

doi:10.2131/jts.34.s121

Cited by 19 publications

(16 citation statements)

References 10 publications

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“…These findings indicate that EGME at 100 mg/kg is likely to have effects on the ovulatory process which may be associated with prolonged tonic blood estrogen levels. In terms of the persistent diestrus at 100 mg/kg, the change was observed from low dose and in mature animals without any effect on body weight (Dodo et al, 2009). It appears that the persistent diestrus at this dose level also can be considered a primary effect of EGME, although this change could be complicated by the secondary effect of decreases in body weight gain.…”

Section: Discussionmentioning

confidence: 96%

“…* P < 0.01, Significant difference compared to control group NC: Not calculated since no animals showed first estrus Prolonged estrous cycle interval: 6 days or longer interval between cycles Persistent estrus: Estrus stage continued 3 days or longer PRL levels (Dodo et al, 2009;Taketa et al, 2011b). The prolonged estrous interval noted in the present study at low dose of 50 mg/kg in pubertal rats was similar to mature female rats; this change was considered to have possibly developed through disruption of the HPG axis during the juvenile to pubertal stages.…”

Section: Discussionmentioning

confidence: 99%

“…EGME was orally administered to juvenile female rats (9 animals/group) for 21 days, at a dose level of 50, 100, or 300 mg/kg from PND 21 to 41. Effective doses on the female reproductive organs were selected based on the relevant literature for the mature female rat (Davis et al, 1997;Dodo et al, 2009;Taketa et al, 2011a). A control group (8 animals) received an equivalent volume of water for injection.…”

Section: Experimental Designmentioning

confidence: 99%

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The effects of ethylene glycol monomethyl ether on female reproductive system in juvenile rats

et al. 2017

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-Ethylene glycol monomethyl ether (EGME), which is widely used in various industrial products, is known for adverse effects on the reproductive system in adult rats. However, the effects of EGME on reproductive development in juvenile rats have not been demonstrated. In order to investigate the effects of EGME on the female reproductive system and pubertal development in juvenile rats, EGME was administered to female Sprague Dawley rats from postnatal day 21 to 41 at a dose level of 0, 50, 100, or 300 mg/kg. The animals were examined for general condition, body weight, vaginal opening (VO), estrous cyclicity, and histopathology of reproductive organs. EGME treatment resulted in a prolonged estrous cycle interval characterized by persistent diestrus at 50 mg/kg without effects on body weight, timing of VO, or histology of the reproductive organs. EGME at 100 mg/kg induced decreases in body weight gain, a delay of VO, and irregular estrous cycle with absence of corpora lutea and hypertrophy of uterine epithelium indicating disturbance of the ovulatory process associated with hormonal effect. At 300 mg/kg, there was significant delay of puberty due to severe growth retardation. The present results revealed that irregular estrous cycle is a first indicator of the effects of EGME on the female reproductive system in juvenile rats, with delayed pubertal onset and ovulatory process disturbance at a higher dose.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

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The effects of ethylene glycol monomethyl ether on female reproductive system in juvenile rats

et al. 2017

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“…30 mg/kg of EGME in the fertility study (Dodo et al 2009). However, Davis et al (1997) reported that EGME had no effect on estrus cyclicity less than 100 mg/kg in a 1-week study.…”

Section: Discussionmentioning

confidence: 98%

Histopathological Characteristics of Luteal Hypertrophy Induced by Ethylene Glycol Monomethyl Ether with a Comparison to Normal Luteal Morphology in Rats

Taketa¹,

Inomata²,

Hosokawa³

et al. 2010

Toxicol Pathol

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Ethylene glycol monomethyl ether (EGME) is a known reproductive toxicant that induces luteal hypertrophy in rat ovaries. In this study, we characterized the histopathological features of corpora lutea (CL) from EGME-treated rats and compared them with normal CL formation and regression. Normally cycling female Sprague-Dawley rats were treated with 5-bromo-2 0 -deoxyuridine (BrdU) intraperitoneally on the morning of estrus and their ovaries were examined 1 (metestrus), 4 (estrus), 8 (estrus), or 12 (estrus) days later to observe the transition of BrdU-labeled cells within in the CL. CL at each time point of estrus stage were classified into 4 types: Type I (newly formed CL), Type II (mature CL), Type III (regressing CL), and Type IV (residual CL). CL almost fully regressed within 4 estrus cycles. In contrast, in female rats given EGME orally (30, 100, or 300 mg/kg for 2 or 4 weeks), luteal cells were hypertrophic with abundant cytoplasm. Although the size of CL varied, all CL in EGME-treated rats had histological features similar to Type II CL, but they were more hypertrophic with less apoptosis. These results suggest that EGME has a luteal hypertrophic effect on all CL phases, including regression.

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“…Because clients who undergo eyelash extension procedures are mostly women of childbearing age, it is necessary to warn women who are or may be pregnant of a risk of miscarriage. 12 It seems necessary to make practitioners always disinfect their fingers and hands as well as devices, provide handling instructions for glues and removers, establish adequate environmental conditions in institutions by providing ventilation or other relevant actions, perform health management for employees, and have ophthalmologic knowledge and knowledge regarding ingredients of glues and removers and their hazards. The current opinion of the Japanese Ministry of Health, Labour and Welfare is expressed in the notification entitled ''Aiming at complete prevention of harm and injury resulting from eyelash extensions,'' dated March 7, 2008, which was issued to the Hygiene Supervision Department of each municipal government, in which it is clearly stated that eyelash extensions should be regarded as a beauty treatment in accordance with the Beauticians Act.…”

Section: Discussionmentioning

confidence: 99%

Ocular Disorders Due to Eyelash Extensions

Amano

Sugimoto

Sugita

2012

Cornea

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Eyelash extension procedures may cause ocular disorders, such as keratoconjunctivitis and allergic blepharitis; indeed, all glues for eyelash extensions analyzed in the present study contained formaldehyde, which can cause keratoconjunctivitis. From the viewpoint of hygienics, it is necessary to disinfect devices, provide handling instructions for organic solvents, improve glue ingredients, and improve the ophthalmologic knowledge of the practitioners.

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Collaborative work on evaluation of ovarian toxicity 11) Two- or four-week repeated-dose studies and fertility study of ethylene glycol monomethyl ether in female rats

Cited by 19 publications

References 10 publications

The effects of ethylene glycol monomethyl ether on female reproductive system in juvenile rats

The effects of ethylene glycol monomethyl ether on female reproductive system in juvenile rats

Histopathological Characteristics of Luteal Hypertrophy Induced by Ethylene Glycol Monomethyl Ether with a Comparison to Normal Luteal Morphology in Rats

Ocular Disorders Due to Eyelash Extensions

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