Collagen and glycosaminoglycans of Wharton's jelly and their alterations in EPH-gestosis

Bańkowski, Edward; Sobolewski, Krzysztof; Romanowicz, Lech; Chyczewski, L; Jaworski, Stefan

doi:10.1016/0301-2115(96)02390-1

Cited by 107 publications

(79 citation statements)

References 15 publications

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“…Furthermore, we reported in previous papers that EPH gestosis is accompanied by a significant increase in sulfated GAGs including heparin and heparan and dermatan sulfates in umbilical cord tissues [10,11]. It is known that these GAGs decrease the affinity of IGFbinding protein-3 for IGF-I.…”

Section: Discussionmentioning

confidence: 99%

“…We have found in our previous studies that the umbilical cord artery contains hyaluronic acid, chondroitin sulfates, dermatan sulfate, heparan sulfate, keratan sulfate and heparin. EPH gestosis results in a premature reduction of hyaluronic acid in the UC artery wall as well as in Wharton's jelly and replacement of hyaluronic acid by sulfated GAGs [10,11].…”

Section: Discussionmentioning

confidence: 99%

“…We found in previous studies that the umbilical cord contains significant amounts of extracellular matrix components, mainly collagen [9,10] and GAGs [10,11]. It is known that extracellular matrix compounds serve as a storage site to concentrate and stabilize growth factors in the vicinity of cells [12].…”

Section: Introductionmentioning

confidence: 99%

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Stimulation of Glycosaminoglycan Biosynthesis by Umbilical Cord Serum of Newborns Delivered by Mothers with EPH Gestosis (Preeclampsia)

Romanowicz¹,

Bańkowski²,

Jaworski

2000

Pathobiology

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Objective: Edema, proteinuria, hypertension (EPH) gestosis, also known as preeclampsia, is the most common, pregnancy-associated pathological syndrome. It is accompanied by a significant increase in collagen content in the umbilical cord arteries and early replacement of hyaluronic acid by sulfated glycosaminoglycans (GAGs) both in these arteries and in Wharton’s jelly. Such a remodelling of the umbilical cord tissues is accompanied by an increase in insulin-like growth factor-I (IGF-I) concentration in the umbilical cord serum. Methods: Contact-inhibited human fibroblasts were incubated in Dulbecco culture medium containing control or EPH umbilical cord serum and supplemented with ¹⁴C-glucosamine or ³⁵S-sulfate. Radioactive GAGs were isolated, submitted to electrophoretic fractionation and quantified. Results: The presence of umbilical cord serum in culture medium strongly stimulated the incorporation of ¹⁴C-glucosamine and ³⁵S-sulfate into GAGs synthesized by these cells. EPH serum was much more active in stimulation of sulfated GAGs biosynthesis than control serum, whereas the biosynthesis of hyaluronic acid was stimulated by both sera to a similar degree. Conclusion: Since IGF-I is known as a stimulator of collagen and sulfated GAG biosynthesis, the high concentration of this growth factor in the umbilical cord plasma may be responsible for preeclampsia-associated remodeling of the umbilical cord.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Stimulation of Glycosaminoglycan Biosynthesis by Umbilical Cord Serum of Newborns Delivered by Mothers with EPH Gestosis (Preeclampsia)

Romanowicz¹,

Bańkowski²,

Jaworski

2000

Pathobiology

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“…WJE is rich in collagen type I (Bańkowski et al, 1996) which could interact and bind to Gal-3 ( Lee and Juliano, 2004). Umbelical cord (UC) Wharton's jelly extract was kindly obtained from Mrs. Bahare Beigi (PhD student, School of Biology, University of Tehran).…”

Section: Extraction Of Wharton's Jelly From Human Umbilical Cord As Smentioning

confidence: 99%

Synergistic Effects of PectaSol-C Modified Citrus Pectin an Inhibitor of Galectin-3 and Paclitaxel on Apoptosis of Human SKOV-3 Ovarian Cancer Cells

Hossein

Keshavarz²,

Ahmadi³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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“…Some investigations have shown that altered composition and metabolism of this organ are observed in some diseases occurring in pregnancy such as fetal growth restriction [1], preeclampsia [2] and diabetes [3].…”

Section: Introductionmentioning

confidence: 99%

Lectin binding in the umbilical cord in altered glycemia

Sgambati

Marini

Thyrion

et al. 2007

European Journal of Obstetrics & Gynecology and Reproductiv

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Objective: Content and distribution of the oligosaccharides in the umbilical cord from pregnancies with altered glycemia were investigated. Study design: A prospective cohort study was conducted in the Florence Policlinic of Careggi, Italy. Samples of cord from physiological pregnancies (n = 20), from pregnancies with minor degree of glucose intolerance (n = 20) and from pregnancies with gestational diabetes mellitus (GDM) treated with insulin (n = 20) were collected. Eleven lectins were used (ConA, WGA, PNA, SBA, DBA, LTA, UEA I, OOA, GSL II, MAL II and SNA) in combination with chemical and enzymatic treatments. Results: Increase of N-acetyl-D-glucosamine and a loss of sialic acid in the umbilical cord of the cases with minor degree of glucose intolerance with respect to the other study groups was observed. D-Galactose(b1 ! 3)-N-acetyl-D-galactosamine, N-acetyl-D-galactosamine and L-fucose were in less amount in both the pathological groups with respect to the control one. Conclusion: The increase of some glycoconjugates carbohydrates and the loss of others in the umbilical cord from pregnancies with minor degree of glucose intolerance might be related to its morphofunctional alterations in a not diabetic altered glycemia. Moreover, the treatment with insulin in the GDM might play a role in restoring partially the normal glycosilation in the cord components in the attempt to renew some their functions. #

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Collagen and glycosaminoglycans of Wharton's jelly and their alterations in EPH-gestosis

Cited by 107 publications

References 15 publications

Stimulation of Glycosaminoglycan Biosynthesis by Umbilical Cord Serum of Newborns Delivered by Mothers with EPH Gestosis (Preeclampsia)

Stimulation of Glycosaminoglycan Biosynthesis by Umbilical Cord Serum of Newborns Delivered by Mothers with EPH Gestosis (Preeclampsia)

Synergistic Effects of PectaSol-C Modified Citrus Pectin an Inhibitor of Galectin-3 and Paclitaxel on Apoptosis of Human SKOV-3 Ovarian Cancer Cells

Lectin binding in the umbilical cord in altered glycemia

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