Collagen as Temporary Dressing and Blood Vessel Replacement

Benjamin, H. B.; Pawlowski, Eugene; Becker, Alan

doi:10.1001/archsurg.1964.01310230001001

Cited by 14 publications

(3 citation statements)

References 2 publications

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“…On the practical side, collagen‐based wound care dressings have the advantage of long‐term storage under sterile conditions. Thus, they may be easily stockpiled for emergent needs . On scientific considerations, although observational studies report favorable outcomes, the underlying mechanisms of action remain speculative and not established in wound healing studies.…”

Section: Discussionmentioning

confidence: 99%

A modified collagen gel enhances healing outcome in a preclinical swine model of excisional wounds

Elgharably

Roy

Khanna

et al. 2013

Wound Repair Regeneration

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Collagen-based dressings are of great interest in wound care. However, evidence supporting their mechanism of action in a wound setting in vivo is scanty. This work providesfirst results from a pre-clinical swine model of excisional wounds elucidating the mechanism of action of a modified collagen gel (MCG) dressing. Following wounding, wound-edge tissue was collected at specific time intervals (3, 7, 14, and 21 days post-wounding). On day 7, histological analysis showed significant increase in the length of rete ridges suggesting improved biomechanical properties of the healing wound tissue. Rapid and transient mounting of inflammation is necessary for efficient healing. MCG significantly accelerated neutrophil and macrophages recruitment to the wound site on day 3 and day 7 with successful resolution of inflammation on day 21. MCG induced MCP-1 expression in neutrophil-like HL-60 cells in vitro. In vivo, MCG treated wound tissue displayed elevated VEGF expression. Consistently, MCG-treated wounds displayed significantly higher abundance of endothelial cells with increased blood flow to the wound area indicating improved vascularization. This observation was explained by the finding that MCG enhanced proliferation of wound-site endothelial cells. In MCG-treated wound tissue, Masson’s Trichrome and Picrosirius red staining showed higher abundance of collagen and increased collagen type I:III ratio. This work presents first evidence from a pre-clinical experimental setting explaining how a collagen-based dressing may improve wound closure by targeting multiple key mechanisms as compared to standard of care i.e., Tegadem treated wounds. The current findings warrant additional studies to determine whether the responses to the MCG are different from other modified or unmodified collagen based products used in clinical setting.

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Section: Discussionmentioning

confidence: 99%

A modified collagen gel enhances healing outcome in a preclinical swine model of excisional wounds

Elgharably

Roy

Khanna

et al. 2013

Wound Repair Regeneration

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“…Other methods of preparing aligned fibrils have been evaluated, including electrical gradients, gravity‐driven flow, and polymerization, etc. Of the different methods assessed, magnetic alignment of the fibrils was superior . Besides application of external forces, alignment of protein fibrils using cell‐induced forces has been explored.…”

Section: Strategic Design Of Ngcmentioning

confidence: 99%

Strategic Design and Fabrication of Nerve Guidance Conduits for Peripheral Nerve Regeneration

Sarker

Naghieh

McInnes

et al. 2018

Biotechnology Journal

150

111

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Nerve guidance conduits (NGCs) have been drawing considerable attention as an aid to promote regeneration of injured axons across damaged peripheral nerves. Ideally, NGCs should include physical and topographic axon guidance cues embedded as part of their composition. Over the past decades, much progress has been made in the development of NGCs that promote directional axonal regrowth so as to repair severed nerves. This paper briefly reviews the recent designs and fabrication techniques of NGCs for peripheral nerve regeneration. Studies associated with versatile design and preparation of NGCs fabricated with either conventional or rapid prototyping (RP) techniques have been examined and reviewed. The effect of topographic features of the filler material as well as porous structure of NGCs on axonal regeneration has also been examined from the previous studies. While such strategies as macroscale channels, lumen size, groove geometry, use of hydrogel/matrix, and unidirectional freeze-dried surface are seen to promote nerve regeneration, shortcomings such as axonal dispersion and wrong target reinnervation still remain unsolved. On this basis, future research directions are identified and discussed.

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“…In addition to this drainage method, several research groups have produced organized layer(s) of collagen fibrils (often with the intention of using them for guiding cell culture systems). Methods employed to influence collagen fibril organization during self-assembly include, electro-spinning [23], the use of strong magnetic fields [24-26], electrical gradients [27], flows through a microfluidic channel [28, 29], a combination of fluid flow and magnetic field [30], dip-pen nanolithography [31], cholesteric methods [32], and even freezing and thawing [33]. Though great progress has been made in controlling collagen organization, in general, the kinetics of collagen polymerization during influenced assembly has generally been neglected.…”

Section: Introductionmentioning

confidence: 99%

Dynamic shear-influenced collagen self-assembly

2009

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The ability to influence the direction of polymerization of a self-assembling biomolecular system has the potential to generate materials with extremely high anisotropy. In biological systems where highly-oriented cellular populations give rise to aligned and often load-bearing tissue such organized molecular scaffolds could aid in the contact guidance of cells for engineered tissue constructs (e.g cornea and tendon). In this investigation we examine the detailed dynamics of pepsin-extracted type I bovine collagen assembly on a glass surface under the influence of flow between two plates. Differential Interference Contrast (DIC) imaging (60x-1.4NA) with focal plane stabilization was used to resolve and track the growth of collagen aggregates on borosilicate glass for 4 different shear rates (500, 80, 20, and 9 s -1 ). The detailed morphology of the collagen fibrils/aggregates was examined using Quick Freeze Deep Etch electron microscopy. Nucleation of fibrils on the glass was observed to occur rapidly (~2 min) followed by continued growth of the fibrils. The growth rates were dependent on flow in a complex manner with the highest rate of axial growth (0.1 microns/sec) occurring at a shear rate of 9 s -1 . The lowest growth rate occurred at the highest shear. Fibrils were observed to both branch and join during the experiments. The best alignment of fibrils was observed at intermediate shear rates of 20 and 80s -1 . However, the investigation revealed that fibril directional growth was not stable. At high shear rates, fibrils would often turn downstream forming what we term "hooks" which are likely the combined result of monomer interaction with the initial collagen layer or "mat" and the high shear rate. Further, QFDE examination of fibril morphology demonstrated that the assembled fibrillar structure did not possess native D-periodicity. Instead, fibrils comprised a collection of generally aligned, monomers which were self-assembled to form a fibril-like aggregate. In conclusion, though constant shear-rate clearly influences collagen fibrillar alignment, the formation of highlyorganized collagenous arrays of native-like D-banded fibrils remains a challenge. Modulation of shear in combination with surface energy patterning to produce a highly-aligned initial mat may provide significant improvement of both the fibril morphology and alignment.

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Collagen as Temporary Dressing and Blood Vessel Replacement

Cited by 14 publications

References 2 publications

A modified collagen gel enhances healing outcome in a preclinical swine model of excisional wounds

A modified collagen gel enhances healing outcome in a preclinical swine model of excisional wounds

Strategic Design and Fabrication of Nerve Guidance Conduits for Peripheral Nerve Regeneration

Dynamic shear-influenced collagen self-assembly

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