Collagen-Based Matrices Improve the Delivery of Transplanted Circulating Progenitor Cells

Zhang, Yan; Thorn, Stephanie; DaSilva, Jean N.; Lamoureux, Marc; deKemp, Robert A.; Beanlands, Rob; Ruel, Marc; Suuronen, Erik J.

doi:10.1161/circimaging.108.781120

Cited by 52 publications

(15 citation statements)

References 31 publications

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“…34 In addition, the low engraftment and survival 35 of transplanted cells limit their benefits. In this regard, we have previously demonstrated by tracking cells with PET and by histology that our collagen-based matrix can improve cell retention and prevent excessive relocation of transplanted cells to nontarget tissues, 36 in addition to improving capillary density. 14 We also previously showed that CACs exposed to the matrix demonstrated higher levels of phosphorylated Akt (PI3K/Akt pathway) and increased survival under hypoxia.…”

Section: Discussionmentioning

confidence: 99%

Preclinical Evaluation of Biopolymer-Delivered Circulating Angiogenic Cells in a Swine Model of Hibernating Myocardium

Giordano

Thorn

Renaud

et al. 2013

Circ: Cardiovascular Imaging

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M yocardial hibernation is a common clinical condition affecting patients with advanced coronary artery disease, 1 for whom cell-based therapies are targeted. In hibernation, repetitive episodes of ischemia and reperfusion lead to metabolic and functional changes in cardiomyocytes, ultimately impairing left ventricular (LV) function. Current therapies such as coronary artery bypass grafting or percutaneous coronary intervention are well established but not suitable for all patients; in previous clinical research from our group, more than one third of patients referred for revascularization did not undergo intervention because of unsuitable vessel anatomy, comorbidities, or other reasons.1 These patients are at high risk of cardiac events, and novel approaches such as cell-based vasculogenic therapy could provide them with an alternative form of therapy. Clinical Perspective on p 991Circulating angiogenic cells (CACs) constitute a heterogeneous population of peripheral blood-derived fibronectincultured cells. Although what defines their phenotype is still debated, 2 they were shown to uptake acetylated low-density lipoprotein, bind Ulex europaeus agglutinin-1 lectin, and express the pan-leukocyte marker CD45. They can also stain positive for monocyte/macrophage (CD14, CD11b/Mac-1, and CD11c) and endothelial (vascular endothelial growth factor receptor 2, von Willebrand factor, vascular endothelialcadherin, and CD31) markers. [3][4][5] Transplanted cells likely contribute to neovascularization through a paracrine mechanism by secreting and recruiting cardioprotective or proangiogenic growth factors. 6,7 The revascularization potential of Background-Vasculogenic cell-based therapy combined with tissue engineering is a promising revascularization approach targeted at patients with advanced coronary artery disease, many of whom exhibit myocardial hibernation. However, to date, no experimental data have been available in this context; we therefore examined the biopolymer-supported delivery of circulating angiogenic cells using a clinically relevant swine model of hibernating myocardium. Methods and Results-Twenty-five swine underwent placement of an ameroid constrictor on the left circumflex artery.After 2 weeks, animals underwent echocardiography, rest and stress ammonia-positron emission tomography perfusion, and fluorodeoxyglucose positron emission tomography viability scans. The following week, swine were randomized to receive intramyocardial injections of PBS control (n=10), circulating angiogenic cells (n=8), or circulating angiogenic cells+collagen-based matrix (n=7). The imaging protocol was repeated after 7 weeks. Baseline positron emission tomography myocardial blood flow and myocardial flow reserve were reduced in the left circumflex artery territory (both P<0.001), and hibernation (mismatch) was observed. At follow-up, stress myocardial blood flow had increased (P≤0.01) and hibernation decreased (P<0.01) in the cells+matrix group only. Microsphere-measured myocardial blood flow validated the perfusion resu...

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Section: Discussionmentioning

confidence: 99%

Preclinical Evaluation of Biopolymer-Delivered Circulating Angiogenic Cells in a Swine Model of Hibernating Myocardium

Giordano

Thorn

Renaud

et al. 2013

Circ: Cardiovascular Imaging

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“…More specifically, no relocated cells were found in the liver of animals receiving this combination treatment, which is, however, normally a sink for the relocation of injected cells [328]. Similarly, another study demonstrated improved early engraftment and directed localization of transplanted endothelial progenitor cells (EPCs) post-transplantation via enhancing progenitor cell retention and limiting distribution to nonspecific tissues when the cells were impregnated in an injectable collagen matrix [329]. ECM-mimetic hydrogels containing the collagen I-derived peptide GPQGIAGQ were also demonstrated to induce endothelial cell adhesion and capillary-like network formation [330].…”

Section: Human Tissue Ecm-based Biomaterialsmentioning

confidence: 99%

Advancing biomaterials of human origin for tissue engineering

Chen

Liu

2016

Progress in Polymer Science

963

513

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Biomaterials have played an increasingly prominent role in the success of biomedical devices and in the development of tissue engineering, which seeks to unlock the regenerative potential innate to human tissues/organs in a state of deterioration and to restore or reestablish normal bodily function. Advances in our understanding of regenerative biomaterials and their roles in new tissue formation can potentially open a new frontier in the fast-growing field of regenerative medicine. Taking inspiration from the role and multi-component construction of native extracellular matrices (ECMs) for cell accommodation, the synthetic biomaterials produced today routinely incorporate biologically active components to define an artificial in vivo milieu with complex and dynamic interactions that foster and regulate stem cells, similar to the events occurring in a natural cellular microenvironment. The range and degree of biomaterial sophistication have also dramatically increased as more knowledge has accumulated through materials science, matrix biology and tissue engineering. However, achieving clinical translation and commercial success requires regenerative biomaterials to be not only efficacious and safe but also cost-effective and convenient for use and production. Utilizing biomaterials of human origin as building blocks for therapeutic purposes has provided a facilitated approach that closely mimics the critical aspects of natural tissue with regard to its physical and chemical properties for the orchestration of wound healing and tissue regeneration. In addition to directly using tissue transfers and transplants for repair, new applications of human-derived biomaterials are now focusing on the use of naturally occurring biomacromolecules, decellularized ECM scaffolds and autologous preparations rich in growth factors/non-expanded stem cells to either target acceleration/magnification of the body's own repair capacity or use nature's paradigms to create new tissues for restoration. In particular, there is increasing interest in separating ECMs into simplified functional domains and/or biopolymeric assemblies so that these components/constituents can be discretely exploited and manipulated for the production of bioscaffolds and new biomimetic biomaterials. Here, following an overview of tissue auto-/allo-transplantation, we discuss the recent trends and advances as well as the challenges and future directions in the evolution and application of human-derived biomaterials for reconstructive surgery and tissue engineering. In particular, we focus on an exploration of the structural, mechanical, biochemical and biological information present in native human tissue for bioengineering applications and to provide inspiration for the design of future biomaterials.

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“…Chitosan and collagen are naturally derived, biodegradable, biocompatible polymers that have been explored for their potential use in tissue engineering [13,[28][29][30][31][32]. To improve upon the resulting hydrogel mechanical properties, it is common for these compounds, either alone or in combination, to be cross-linked using exogenous (occasionally toxic) chemical cross-linkers [28,33,34].…”

Section: Hydrogel Formationmentioning

confidence: 99%

Modifications of collagen-based biomaterials with immobilized growth factors or peptides

Xiao

Reis

Zhao

et al. 2015

Methods

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Collagen-Based Matrices Improve the Delivery of Transplanted Circulating Progenitor Cells

Abstract: Background-Collagen

Cited by 52 publications

References 31 publications

Preclinical Evaluation of Biopolymer-Delivered Circulating Angiogenic Cells in a Swine Model of Hibernating Myocardium

Preclinical Evaluation of Biopolymer-Delivered Circulating Angiogenic Cells in a Swine Model of Hibernating Myocardium

Advancing biomaterials of human origin for tissue engineering

Modifications of collagen-based biomaterials with immobilized growth factors or peptides

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