Collagen biomaterial doped with colominic acid for cell culture applications with regard to peripheral nerve repair

Bruns, Stephanie; Stark, Yvonne; Röker, Stefanie; Wieland, Martin; Dräger, Gerald; Kirschning, Andreas; Stahl, Frank; Kasper, Cornelia; Scheper, Thomas

doi:10.1016/j.jbiotec.2007.06.024

Cited by 19 publications

(13 citation statements)

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“…Certainly, this drawback will be solved after development of solid polySia-based scaffold materials for which a prolonged regeneration promoting effect can be expected. 38 Autotransplantation and SCþK1-polySia grafts did not account for substantial functional recovery across a 13 mm nerve gap. This is in contrast to the recent demonstration that polySia-mimicking peptides promote structural as well as functional recovery across short, 2 mm, femoral nerve gaps in mice.…”

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confidence: 91%

In Vivo Evaluation of Polysialic Acid as Part of Tissue-Engineered Nerve Transplants

Haastert‐Talini

Schaper-Rinkel²,

Schmitte³

et al. 2010

Tissue Engineering Part A

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With the aim to develop new biomaterials for peripheral nerve grafts, the current study used bioidentical polysialic acid (polySia) as complement in synthetic conduits. polySia provides an important guidance cue during nervous system development and regeneration. First in vivo results on the use of cell-free and Schwann cell-containing synthetic peripheral nerve grafts complemented with soluble exogenous K1-polySia are presented. Reconstructing 10 mm rat sciatic nerve gaps, K1-polySia complementation significantly improved structural nerve regeneration in comparison to cell-free and K1-polySia-free grafts. Subsequently, long nerve gaps (13 mm) were reconstructed by Schwann cell transplants plus K1-polySia and compared to nerve autotransplantation. Structural but also functional regeneration could be observed using K1-polySia transplants; however, autotransplantation was still significantly more successful. Overall, the current study demonstrates that exogenous K1-polySia has no negative but rather regeneration promoting effects. This is important novel evidence on the applicability of exogenous polySia in vivo. Further studies are required to develop solid three-dimensional polySia-based scaffolds for nerve tissue engineering. Biocompatible and assessable biodegrading materials will ensure long-lasting presence of polySia to allow its applicability and prolonged efficacy in the slow regenerating scenario of human peripheral nerve reconstruction.

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mentioning

confidence: 91%

In Vivo Evaluation of Polysialic Acid as Part of Tissue-Engineered Nerve Transplants

Haastert‐Talini

Schaper-Rinkel²,

Schmitte³

et al. 2010

Tissue Engineering Part A

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“…Traumatic injuries can damage both skeletal muscle and peripheral nerves, resulting in loss of function at neuromuscular junctions . Both skeletal muscle and peripheral nerves have a limited capacity to regenerate proportional to the amount of tissue loss . After skeletal muscle injury, satellite cells become activated, proliferate, and fuse to form multinucleated myotubes to repair the damaged site.…”

Section: Introductionmentioning

confidence: 99%

Poly(3,4‐ethylenedioxythiophene) nanoparticle and poly(ɛ‐caprolactone) electrospun scaffold characterization for skeletal muscle regeneration

Fischer

Browe

Olabisi

et al. 2015

J Biomedical Materials Res

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Injuries to peripheral nerves and/or skeletal muscle can cause scar tissue formation and loss of function. The focus of this article is the creation of a conductive, biocompatible scaffold with appropriate mechanical properties to regenerate skeletal muscle. Poly(3,4-ethylenedioxythiophene) (PEDOT) nanoparticles (Np) were electrospun with poly(ɛ-caprolactone) (PCL) to form conductive scaffolds. During electrospinning, ribboning, larger fiber diameters, and unaligned scaffolds were observed with increasing PEDOT amounts. To address this, PEDOT Np were sonicated prior to electrospinning, which resulted in decreased conductivity and increased mechanical properties. Multi-walled carbon nanotubes (MWCNT) were added to the 1:2 solution in an effort to increase conductivity. However, the addition of MWCNT had little effect on scaffold conductivity, and the elastic modulus and yield stress of the scaffold increased as a result. Rat muscle cells attached and were active on the 1-10, 1-2, 3-4, and 1-1 PCL-PEDOT scaffolds; however, the 3-4 scaffolds had the lowest level of metabolic activity. Although the scaffolds were cytocompatible, further development of the fabrication method is necessary to produce more highly aligned scaffolds capable of promoting skeletal muscle cell alignment and eventual regeneration.

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“…Some of these proteins have been used in clinical studies, and their functions were observed to be similar to those of PEGylation. PSA may also be employed in nerve cell tissue engi neering because of the similarity of its structure to NCAM [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and characterization of polysialic acid/carboxymethyl chitosan hydrogel with potential for drug delivery

Zhan

Zheng

et al. 2015

Russ J Bioorg Chem

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A novel hydrogel was prepared from polysialic acid (PSA) and carboxymethyl chitosan (CMCS) using glutaraldehyde as the cross-linking agent. The resulting PSA-CMCS hydrogel exhibited pH sensitivity, in which the swelling ratio under acidic conditions was higher than those under neutral or alkaline conditions. The swelling ratio of PSA-CMCS hydrogel at equilibrium depended on the medium pH, the cross-linking agent concentration, and the ratio of PSA to CMCS (w/w). Bovine serum albumin (BSA) and 5-fluorouracil (5-FU) were used as model drugs to prepare hydrogel delivery systems. The loading efficiencies of the hydrogel for BSA and 5-FU were 26.25 and 36.74%, respectively. Release behaviors of BSA and 5-FU were influenced by the pH. MTT assays confirmed that PSA-CMCS hydrogel has no cytotoxicity toward the NIH-3T3 cell line; in fact, the 100% aqueous extract of the PSA-CMCS hydrogel enhanced cell growth. These results suggest that PSA-CMCS hydrogel may be a promising pH-sensitive delivery system, especially for hydrophobic chemicals.

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Collagen biomaterial doped with colominic acid for cell culture applications with regard to peripheral nerve repair

Cited by 19 publications

References 50 publications

In Vivo Evaluation of Polysialic Acid as Part of Tissue-Engineered Nerve Transplants

In Vivo Evaluation of Polysialic Acid as Part of Tissue-Engineered Nerve Transplants

Poly(3,4‐ethylenedioxythiophene) nanoparticle and poly(ɛ‐caprolactone) electrospun scaffold characterization for skeletal muscle regeneration

Synthesis and characterization of polysialic acid/carboxymethyl chitosan hydrogel with potential for drug delivery

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