Collagen Extraction from Recent and Fossil Bones: Quantitative and Qualitative Aspects

Reanalysis of Tyrannosaurus rex Mass Spectra

“…Hemoglobin and collagen are plausible proteins to find in fossil bone, because they are two of the most abundant proteins in bone and bone marrow. Schweitzer et al [15] previously reported multiple lines of evidence, including immunological reactions, for hemoglobin-derived compounds in T. rex bone, and collagen from younger fossil bones is well known [17]. Contamination remains a tricky and possibly unresolvable issue for this particular sample.…”

Evaluating bone collagen extraction methods for stable isotope analysis in dietary studies

“…Several techniques have been developed to prepare bone samples for isotope analysis. Most of these consider and adjust for factors such as humic acids and lipids that might influence the reproducibility of the measurements Brown et al, 1988;Collins and Galley, 1998;Garvie-Lok et al, 2004;Lidén et al, 1995;Nielsen-Marsh and Hedges, 2000;Semal and Orban, 1995).…”

Suitability of Immunohistochemistry for the Determination of Collagen Stability in Historic Bone Tissue

“…Additionally, she did not try to identify collagen break-down fragments-for example by immunoblotting-which may have been in the pool of ''NCPs''. In summary, there is still a demand for careful and extensive biochemical analyses and the recent analysis by Semal & Orban (1995) underlines the importance of solubilization and therefore analysis techniques.…”