Collagen fibre implant for tendon and ligament biological augmentation. In vivo study in an ovine model

Enea, Davide; Gwynne, J.H.; Kew, Simon J.; Arumugam, Meera; Shepherd, Jennifer H.; Brooks, Roger A.; Ghose, Siddhartha; Best, Serena M.; Cameron, Ruth E.; Rushton, Neil

doi:10.1007/s00167-012-2102-7

Cited by 48 publications

(43 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further, in a subcutaneous model, none of the substrates induced cellular orientation parallel to the direction of the substrate topography. It is tempting to hypothesise that two-dimensional imprinted substrates are overwhelmed with body fluids and protein adsorption upon implantation, prohibiting favourable cell / material interaction at the substrate-tissue nano-biointerface and that three-dimensional fibrous constructs are more effective for directional neural [77][78][79], tendon [29,35,80], bone [81][82][83] and skin [84][85][86] neotissue formation and promote relatively enhanced cell growth, motility, matrix deposition and neotissue growth through the provision of a true three-dimensional environment.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Substrate topography: A valuable in vitro tool, but a clinical red herring for in vivo tenogenesis

et al. 2015

View full text Add to dashboard Cite

This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractControlling the cell-substrate interactions at the bio-interface is becoming an inherent element in the design of implantable devices. Modulation of cellular adhesion in vitro, through topographical cues, is a well-documented process that offers control over subsequent cellular functions. However, it is still unclear whether surface topography can be translated into a clinically functional response in vivo at the tissue / device interface. Herein, we demonstrated that anisotropic substrates with a groove depth of ~317 nm and ~1,988 nm promoted human tenocyte alignment parallel to the underlying topography in vitro. However, the rigid poly(lactic-co-glycolic acid) substrates used in this study upregulated the expression of chondrogenic and osteogenic genes, indicating possible tenocyte trans-differentiation. Of significant importance is that none of the topographies assessed (~37 nm, ~317 nm and ~1,988 nm groove depth) induced extracellular matrix orientation parallel to the substrate orientation in a rat patellar tendon model. These data indicate that two-dimensional imprinting technologies are useful tools for in vitro cell phenotype maintenance, rather than for organised neotissue formation in vivo, should multifactorial approaches that consider both surface topography and substrate rigidity be established.

show abstract

Section: Discussionmentioning

confidence: 99%

“…electro-spinning [26][27][28][29][30][31], fibre extrusion [32][33][34][35], isoelectric focusing [36,37] and imprinting [38][39][40]) have been at the forefront of scientific and technological research and innovation to recapitulate native tendon extracellular matrix (ECM) supramolecular assemblies. Although fibrous constructs (e.g.…”

Section: Introductionmentioning

confidence: 99%

Substrate topography: A valuable in vitro tool, but a clinical red herring for in vivo tenogenesis

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Further in vitro analysis demonstrated that such materials, largely attributed to their surface features, not only facilitate bidirectional cell alignment, but also maintain tenogenic phenotype in vitro [360]. Further, preclinical experimentation in peripheral nerve repair and in tendon small and large animal models enhanced the clinical potential of these fibres, as judged by improved structural alignment and biomechanics [361][362][363]. Using an isoelectric focusing setup, aligned collagen fibres have been produced with ultrastructural characteristics and physical properties similar to native tendon [364,365].…”

Section: Bottom-up Approached For Tendon Repair Based On Natural In Omentioning

confidence: 99%

The past, present and future in scaffold-based tendon treatments

Lomas

Ryan

Sorushanova

et al. 2015

Advanced Drug Delivery Reviews

183

188

View full text Add to dashboard Cite

“…The scaffold was incorporated within the native tendon, in concert with our results. Similarly, Enea et al 52 worked with a patellar tendon defect in a sheep model and used an experimental protocol similar to ours, but with a different type of collagen implant, and they found that it was incorporated into the repaired tissue after 3-6 months.…”

mentioning

confidence: 75%

Implantation of a Novel Biologic and Hybridized Tissue Engineered Bioimplant in Large Tendon Defect: AnIn VivoInvestigation

Oryan

Moshiri

Parizi

et al. 2013

Tissue Engineering Part A

View full text Add to dashboard Cite

Surgical reconstruction of large Achilles tendon defects is technically demanding. There is no standard method, and tissue engineering may be a valuable option. We investigated the effects of 3D collagen and collagenpolydioxanone sheath (PDS) implants on a large tendon defect model in rabbits. Ninety rabbits were divided into three groups: control, collagen, and collagen-PDS. In all groups, 2 cm of the left Achilles tendon were excised and discarded. A modified Kessler suture was applied to all injured tendons to retain the gap length. The control group received no graft, the treated groups were repaired using the collagen only or the collagen-PDS prostheses. The bioelectrical characteristics of the injured areas were measured at weekly intervals. The animals were euthanized at 60 days after the procedure. Gross, histopathological and ultrastructural morphology and biophysical characteristics of the injured and intact tendons were investigated. Another 90 pilot animals were also used to investigate the inflammatory response and mechanism of graft incorporation during tendon healing. The control tendons showed severe hyperemia and peritendinous adhesion, and the gastrocnemius muscle of the control animals showed severe atrophy and fibrosis, with a loose areolar connective tissue filling the injured area. The tendons receiving either collagen or collagen-PDS implants showed lower amounts of peritendinous adhesion, hyperemia and muscle atrophy, and a dense tendon filled the defect area. Compared to the control tendons, application of collagen and collagen-PDS implants significantly improved water uptake, water delivery, direct transitional electrical current and tissue resistance to direct transitional electrical current. Compared to the control tendons, both prostheses showed significantly increased diameter, density and alignment of the collagen fibrils and maturity of the tenoblasts at ultrastructure level. Both prostheses influenced favorably tendon healing compared to the control tendons, with no significant differences between collagen and collagen-PDS groups. Implantation of the 3D collagen and collagen-PDS implants accelerated the production of a new tendon in the defect area, and may become a valuable option in clinical practice.

show abstract

Collagen fibre implant for tendon and ligament biological augmentation. In vivo study in an ovine model

Cited by 48 publications

References 36 publications

Substrate topography: A valuable in vitro tool, but a clinical red herring for in vivo tenogenesis

Substrate topography: A valuable in vitro tool, but a clinical red herring for in vivo tenogenesis

The past, present and future in scaffold-based tendon treatments

Implantation of a Novel Biologic and Hybridized Tissue Engineered Bioimplant in Large Tendon Defect: AnIn VivoInvestigation

Contact Info

Product

Resources

About