Collagen fibrillar networks as skeletal frameworks. A demonstration by cell-maceration/scanning electron microscope method.

Ohtani, Osamu; Ushiki, Tatsuo; Taguchi, Takehito; Kikuta, Akio

doi:10.1679/aohc.51.249

Cited by 205 publications

(130 citation statements)

References 25 publications

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“…The present study was conducted by the cellmaceration/SEM method (OHTANI, 1987, OHTANI et al, 1988 which allows a three-dimensional demonstration of individual collagen fibrils in their natural form and location. This paper shows threedimensionally the elaborate network of the fibrils which constitutes the skeletal framework of the liver.…”

Section: Discussionmentioning

confidence: 99%

Three-dimensional organization of the collagen fibrillar framework of the human and rat livers.

Ohtani

1988

Archives of Histology and Cytology

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Section: Discussionmentioning

confidence: 99%

Three-dimensional organization of the collagen fibrillar framework of the human and rat livers.

Ohtani

1988

Archives of Histology and Cytology

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“…Ultrathin sections were analyzed in Philips CM-10 electron microscope (Philips, Amsterdam, The Netherlands). Scanning electron microscopy was performed on whole-mount specimens prepared by alkali maceration (41) and analyzed in a Philips 515 electron microscope. For quantification of matrix amount, the images were converted to white and black pixels that represented matrix and empty areas, respectively.…”

Section: Methodsmentioning

confidence: 99%

Collagen-binding proteoglycan fibromodulin can determine stroma matrix structure and fluid balance in experimental carcinoma

Oldberg¹,

Kalamajski²,

Salnikov

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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Research on the biology of the tumor stroma has the potential to lead to development of more effective treatment regimes enhancing the efficacy of drug-based treatment of solid malignancies. Tumor stroma is characterized by distorted blood vessels and activated connective tissue cells producing a collagen-rich matrix, which is accompanied by elevated interstitial fluid pressure (IFP), indicating a transport barrier between tumor tissue and blood. Here, we show that the collagen-binding proteoglycan fibromodulin controls stroma structure and fluid balance in experimental carcinoma. Gene ablation or inhibition of expression by antiinflammatory agents showed that fibromodulin promoted the formation of a dense stroma and an elevated IFP. Fibromodulindeficiency did not affect vasculature but increased the extracellular fluid volume and lowered IFP. Our data suggest that fibromodulin controls stroma matrix structure that in turn modulates fluid convection inside and out of the stroma. This finding is particularly important in relation to the demonstration that targeted modulations of the fluid balance in carcinoma can increase the response to cancer therapeutic agents.physiology ͉ TGF-␤ ͉ tumor physiology ͉ inflammation ͉ interstitial fluid pressure

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“…The cell-maceration method of Ohtani et al (1988) was utilized. After initial fixation in 3 % glutaraldehyde, right soleus muscles were cut into 3×3×3 mm blocks and immersed in 10 % aqueous sodium hydroxide solution for 14 d at room temperature; subsequently, tissues were rinsed in distilled water for 3 d. These samples were placed in a 1 % aqueous tannic acid solution for 2 h and rinsed in distilled water for 1 h, followed by postfixing in a 1 % aqueous solution of osmium tetroxide for 2 h, after which they were dehydrated in a series of graded concentrations of ethanol and dried by the t-butyl alcohol freeze-drying method (Inoue and Osatake, 1988).…”

Section: Morphological Analysis Of the Endomysiummentioning

confidence: 99%

Effects of Therapeutic Ultrasound on Joint Mobility and Collagen Fibril Arrangement in the Endomysium of Immobilized Rat Soleus Muscle

Okita

Nakano

Kataoka³

et al. 2009

Ultrasound in Medicine & Biology

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This study examined effects of therapeutic ultrasound on joint mobility and collagen fibril arrangement in the endomysium of immobilized rat soleus muscle.Twenty-two male Wistar rats were divided randomly into control (n=5) and experimental groups (n=17).In the experimental group, bilateral ankle joints of each rat were fixed in full plantar flexion with a plaster cast over a 4-wk period.Five animals in the experimental group were immobilized throughout the 4-wk (immobilization group) period, whereas the remaining rats in the experimental group were randomly divided into the ultrasound (US, n=6) and sham (n=6) treatment groups.Under anesthesia, continuous ultrasonic energy (frequency, 1 MHz; intensity, 1.0 W/cm 2 ) was delivered to the triceps surae muscle of the US group for 15 min per d, 6 d per wk over the 4-wk immobilization period. Ultrasonic energy was not delivered to the triceps surae muscle in sham animals; only the transducer head was moved.Ankle joint mobility on dorsiflexion in the immobilization, sham and US groups was significantly smaller than that of the control group, whereas in the US group, this parameter was significantly greater than in the immobilization and sham groups.Collagen fibril arrangement in the endomysium of the control and US groups was longitudinal to the axis of the muscle fibers; in contrast, it was circumferential in the immobilization and sham groups. Our findings revealed that joint immobilization induces decreased joint mobility and collagen fibril movement in the endomysium; 3 furthermore, ultrasound treatment can prevent these changes. We hypothesized that therapeutic ultrasound during the immobilization process may inhibit deterioration of muscle contracture.

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Collagen fibrillar networks as skeletal frameworks. A demonstration by cell-maceration/scanning electron microscope method.

Cited by 205 publications

References 25 publications

Three-dimensional organization of the collagen fibrillar framework of the human and rat livers.

Three-dimensional organization of the collagen fibrillar framework of the human and rat livers.

Collagen-binding proteoglycan fibromodulin can determine stroma matrix structure and fluid balance in experimental carcinoma

Effects of Therapeutic Ultrasound on Joint Mobility and Collagen Fibril Arrangement in the Endomysium of Immobilized Rat Soleus Muscle

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