Collagen filaments as a scaffold for nerve regeneration

Yoshii, Satoru; Oka, M.

doi:10.1002/1097-4636(20010905)56:3<400::aid-jbm1109>3.0.co;2-7

Cited by 91 publications

(68 citation statements)

References 25 publications

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“…In addition, the simultaneous immobilization of bioactive substances is feasible. Our previous study 35, [37][38][39]. The bioactive substances used are laminin and fibronectin, which are cell adhesion proteins and showed that the release of bioactive substances from photogels depends on the degree of swelling of the phowhich accelerate cell migration and proliferation (7,11,17,20,22,30,31,37,38).…”

Section: Electrophysiological Responsesmentioning

confidence: 99%

“…Therefore, longitudinally directed growth (10,18,20,27,29,30-32, 38,39). Collagen has been used to fabricate such neurothe combined use of a high surface area and bioactive substances may provide an adequate microenvironment prosthetic devices (6, 16,20,30,31,39).…”

Section: Electrophysiological Responsesmentioning

confidence: 99%

“…Our approach was to utilize photocurable gelatin to conducive to guided axon migration and growth over appreciable distances (16,20,30,(37)(38)(39). fabricate such devices.…”

Section: Electrophysiological Responsesmentioning

confidence: 99%

“…(7,11,17,20,37,38) and fibronectin (7,11,37) in a tubular Tokyo, Japan; maximum wavelength of illumination, 420-430 nm; and light intensity, 1.5 × 10 6 lx) for 15 min conduit. The other is to incorporate multiple contact guide artificial neurofilaments (20,22,(29)(30)(31)38,39) or using a custom-designed equipment. The photocured gelatin nerve conduit had the following dimensions: inmultimicroporous foams into a nerve conduit (10,18,27,36).…”

Section: Introductionmentioning

confidence: 99%

“…Such a structurally integral tubular conduit packed ner diameter, 1.2 mm; outer diameter, 2.4 mm; wall thickness, 0.6 mm; and length, 15 mm. The 40 wt% photowith multiple guidance fibers (22,30,31,39) or a sponge impregnated with extracellular matrix proteins or growth cured gelatin fibers, which were impregnated with bioactive substances (1 mg of laminin, 1 mg of fibronectin; factors (31), and incorporated with or without of Schwann cells (1,9,34) when placed between the severed ends, Becton Dickinson Labware, Bedford, MA), and 100 µg of nerve growth factor (Invitrogen Life Technologies, had a profound effect on nerve regeneration by orienting neurotrophin diffusion, matrix formation, and cellular Carlsbad, CA) had diameters ranging from 10 to 100 µm and a length of 10 mm. The following three types and axonal growth within the tube, thereby enhancing regeneration.…”

Section: Introductionmentioning

confidence: 99%

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Photofabricated Gelatin-Based Nerve Conduits: Nerve Tissue Regeneration Potentials

et al. 2004

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There is a strong demand for development of nerve guide conduit with prompt nerve regeneration potential for injury-induced nerve defect. Prior to study on nerve tissue engineering using Schwann cells or nerve stem cells, the effectiveness of photofabricated scaffolds based on photocurable gelatin was examined. This study describes the evaluation of in vivo nerve tissue regeneration potentials of three custom-designed and -fabricated prostheses (inner diameter, 1.2 mm; outer diameter, 2.4 mm; wall thickness, 0.60 mm; and length, 15 mm) made of photocured gelatin: a plain photocured gelatin tube (model I), a photocured gelatin tube packed with bioactive substances (laminin, fibronectin, and nerve growth factor) coimmobilized in a photocured gelatin rod (model II), and a photocured gelatin tube packed with bioactive substances coimmobilized in multifilament fibers (model III). These prostheses were implanted between the proximal and distal stumps 10 mm of the dissected right sciatic nerve of 70 adult male Lewis rats for up to 1 year. The highest regenerative potentials were found using the model III prosthesis, followed by the model II prosthesis. Markedly retarded neural regeneration was observed using the model I prosthesis. These were evaluated from the viewpoints of functional recovery, electrophysiological responses, and tissue morphological regeneration. The significance of the synergistic cooperative functions of multifilaments, which serve as a platform that provides contact guidance to direct longitudinal cell movement and tissue ingrowth and as a cell adhesive matrix with high surface area, and immobilized bioactive substances, which enhance nerve regeneration via biological stimulation, is discussed.

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Section: Electrophysiological Responsesmentioning

confidence: 99%

Section: Electrophysiological Responsesmentioning

confidence: 99%

“…Our approach was to utilize photocurable gelatin to conducive to guided axon migration and growth over appreciable distances (16,20,30,(37)(38)(39). fabricate such devices.…”

Section: Electrophysiological Responsesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Photofabricated Gelatin-Based Nerve Conduits: Nerve Tissue Regeneration Potentials

et al. 2004

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Biomimetic Architectures for Peripheral Nerve Repair: A Review of Biofabrication Strategies

Wieringa

Pinho

Micera

et al. 2018

Adv Healthcare Materials

124

116

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Biofabrication techniques have endeavored to improve the regeneration of the peripheral nervous system (PNS), but nothing has surpassed the performance of current clinical practices. However, these current approaches have intrinsic limitations that compromise patient care. The "gold standard" autograft provides the best outcomes but requires suitable donor material, while implantable hollow nerve guide conduits (NGCs) can only repair small nerve defects. This review places emphasis on approaches that create structural cues within a hollow NGC lumen in order to match or exceed the regenerative performance of the autograft. An overview of the PNS and nerve regeneration is provided. This is followed by an assessment of reported devices, divided into three major categories: isotropic hydrogel fillers, acting as unstructured interluminal support for regenerating nerves; fibrous interluminal fillers, presenting neurites with topographical guidance within the lumen; and patterned interluminal scaffolds, providing 3D support for nerve growth via structures that mimic native PNS tissue. Also presented is a critical framework to evaluate the impact of reported outcomes. While a universal and versatile nerve repair strategy remains elusive, outlined here is a roadmap of past, present, and emerging fabrication techniques to inform and motivate new developments in the field of peripheral nerve regeneration.

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Tunable Enzymatically Cross‐Linked Silk Fibroin Tubular Conduits for Guided Tissue Regeneration

Carvalho

Costa

Morais

et al. 2018

Adv Healthcare Materials

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Hollow tubular conduits (TCs) with tunable architecture and biological properties are in great need for modulating cell functions and drug delivery in guided tissue regeneration. Here, a new methodology to produce enzymatically cross-linked silk fibroin TCs is described, which takes advantage of the tyrosine groups present in silk structure that are known to allow the formation of a covalently cross-linked hydrogel. Three different processing methods are used as a final step to modulate the properties of the silk-based TCs. This approach allows to virtually adjust any characteristic of the final TCs. The final microstructure ranges from a nonporous to a highly porous network, allowing the TCs to be selectively porous to 4 kDa molecules, but not to human skin fibroblasts. Mechanical properties are dependent both on the processing method and thickness of the TCs. Bioactivity is observed after 30 days of immersion in simulated body fluid only for the TCs submitted to a drying processing method (50 °C). The in vivo study performed in mice demonstrates the good biocompatibility of the TCs. The enzymatically cross-linked silk fibroin TCs are versatile and have adjustable characteristics that can be exploited in a variety of biomedical applications, particularly in guidance of peripheral nerve regeneration.

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Collagen filaments as a scaffold for nerve regeneration

Cited by 91 publications

References 25 publications

Photofabricated Gelatin-Based Nerve Conduits: Nerve Tissue Regeneration Potentials

Photofabricated Gelatin-Based Nerve Conduits: Nerve Tissue Regeneration Potentials

Biomimetic Architectures for Peripheral Nerve Repair: A Review of Biofabrication Strategies

Tunable Enzymatically Cross‐Linked Silk Fibroin Tubular Conduits for Guided Tissue Regeneration

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