Collagen Hydrogel Scaffold and Fibroblast Growth Factor-2 Accelerate Periodontal Healing of Class II Furcation Defects in Dog

Abstract

Objective:Collagen hydrogel scaffold exhibits bio-safe properties and facilitates periodontal wound healing. However, regenerated tissue volume is insufficient. Fibroblast growth factor-2 (FGF2) up-regulates cell behaviors and subsequent wound healing. We evaluated whether periodontal wound healing is promoted by application of collagen hydrogel scaffold in combination with FGF2 in furcation defects in beagle dogs.Methods:Collagen hydrogel was fabricated from bovine type I collagen with an ascorbate-copper ion cross-linking system. Collagen hydrogel was mingled with FGF2 and injected into sponge-form collagen. Subsequently, FGF2 (50 µg)/collagen hydrogel scaffold and collagen hydrogel scaffold alone were implanted into class II furcation defects in dogs. In addition, no implantation was performed as a control. Histometric parameters were assessed at 10 days and 4 weeks after surgery.Result:FGF2 application to scaffold promoted considerable cell and tissue ingrowth containing numerous cells and blood vessel-like structure at day 10. At 4 weeks, reconstruction of alveolar bone was stimulated by implantation of scaffold loaded with FGF2. Furthermore, periodontal attachment, consisting of cementum-like tissue, periodontal ligament-like tissue and Sharpey’s fibers, was also repaired, indicating that FGF2-loaded scaffold guided self-assembly and then re-established the function of periodontal organs. Aberrant healing, such as ankylosis and root resorption, was not observed.Conclusion:FGF2-loaded collagen hydrogel scaffold possessed excellent biocompatibility and strongly promoted periodontal tissue engineering, including periodontal attachment re-organization.

Collagen hydrogels loaded with fibroblast growth factor-2 as a bridge to repair brain vessels in organotypic brain slices

“…Collagen gels together with FGF-2 treatment provided higher survival of embedded endothelial cells and enhanced vessel branching from embedded vessel fragments (Satake et al 1998 ; Nicosia et al 2005 ). Collagen hydrogels loaded with FGF-2 were used before incorporated into collagen sponges in periodontal wound healing and provided early ingrowth of vessel-like structures besides promoting healing in different tissue types (Momose et al 2016 ). In the light of these studies, collagen hydrogels can be a useful tool for providing an extracellular matrix for endothelial cell proliferation and migration, as well as a modifiable platform for delivering angiogenic factors.…”

Accelerated construction of an in vitro model of human periodontal ligament tissue: vacuum plasma combined with fibronectin coating and a polydimethylsiloxane matrix

“…After gaining polarity, both groups maintained it until day 7, which suggested that this model can maintain maturity until at least day 7 (consistent with the PCR result). FGF-2 is an important growth factor that enhances PDL regeneration (Momose et al, 2016). Both PN and PF groups showed similar increase and decrease patterns for FGF-2 and OCN , but without statistically significant differences under most circumstances.…”

Using Mesenchymal Stem Cells to Treat Female Infertility: An Update on Female Reproductive Diseases

“…Several studies have shown that MB-MSCs reduce apoptosis in granulosa cells and fibrosis of the ovarian interstitium, thereby improving folliculogenesis and rescuing overall ovarian function in an animal model of POF [58, 59], including restoring fertility [60]. In addition, Wang et al demonstrated that MB-MSCs produced a high level of fibroblast growth factor 2, which enhanced cell survival, proliferation, and function to repair tissue damage [61, 62]. Furthermore, Yan et al indicated that MB-MSCs reduced granulosa cell apoptosis and improved ovarian functions in mice by downregulating Gadd45b protein expression (a stress sensor whose effects are mediated via physical interactions with other cellular proteins implicated in cell cycle regulation) and upregulating cyclinB1 and CDC2 (regulators of the G2/M transition in mammalian cells) [63–66].…”