Collagen Hydrolysate as Sustainable Additive for Cereal Seed Treatment

Gaidău, Carmen; Niculescu, Mihaela; Epure, Doru Gabriel; Tudorache, Mădălina; Radu, Elena; Stoica, Rusăndica; Ignat, Madalina; Gidea, Mihai

doi:10.37358/rc.17.12.5978

Cited by 4 publications

(7 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The authors concluded that these genes, and their possible transcriptional regulation through the two transcription factors, could be an important mechanism regulating improved plant growth following gelatin seed treatment. The use of collagen hydrolysate in wheat seed treatment showed a stimulation of seed metabolism by increasing endogenous gibberellic acid, and an enhancement of emergence and seedling biomass, and a reduction of abnormal seedlings ( Gaidau et al, 2015 ). Furthermore, Gaidau et al (2013) showed that cereal seed treatments with collagen-based hydrolysate mixes with fungicides and insecticides reduced pesticide needs, with diminished environmental impact and reduced cost of seed treatment.…”

Section: Effects On Physiological and Agronomic Traits Of Cropsmentioning

confidence: 99%

Biostimulant Action of Protein Hydrolysates: Unraveling Their Effects on Plant Physiology and Microbiome

et al. 2017

View full text Add to dashboard Cite

Plant-derived protein hydrolysates (PHs) have gained prominence as plant biostimulants because of their potential to increase the germination, productivity and quality of a wide range of horticultural and agronomic crops. Application of PHs can also alleviate the negative effects of abiotic plant stress due to salinity, drought and heavy metals. Recent studies aimed at uncovering the mechanisms regulating these beneficial effects indicate that PHs could be directly affecting plants by stimulating carbon and nitrogen metabolism, and interfering with hormonal activity. Indirect effects could also play a role as PHs could enhance nutrient availability in plant growth substrates, and increase nutrient uptake and nutrient-use efficiency in plants. Moreover, the beneficial effects of PHs also could be due to the stimulation of plant microbiomes. Plants are colonized by an abundant and diverse assortment of microbial taxa that can help plants acquire nutrients and water and withstand biotic and abiotic stress. The substrates provided by PHs, such as amino acids, could provide an ideal food source for these plant-associated microbes. Indeed, recent studies have provided evidence that plant microbiomes are modified by the application of PHs, supporting the hypothesis that PHs might be acting, at least in part, via changes in the composition and activity of these microbial communities. Application of PHs has great potential to meet the twin challenges of a feeding a growing population while minimizing agriculture’s impact on human health and the environment. However, to fully realize the potential of PHs, further studies are required to shed light on the mechanisms conferring the beneficial effects of these products, as well as identify product formulations and application methods that optimize benefits under a range of agro-ecological conditions.

show abstract

Section: Effects On Physiological and Agronomic Traits Of Cropsmentioning

confidence: 99%

Biostimulant Action of Protein Hydrolysates: Unraveling Their Effects on Plant Physiology and Microbiome

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The main physical-chemical characteristics of collagen hydrolysate (HC10) before and after concentration (HC10CC), RCG and KH extracts are shown in Table 2. [36], and its viscosity raised up to 615 cP (Table 2), which makes it an interesting material for electrospinning. The conductivity of KH shows the highest value due to the high content in salts, which influences the particle size and stability.…”

Section: Characterization Of Hc10 Hc10cc Rcg and Kh Extractsmentioning

confidence: 99%

New Nanofibers based on Protein By-Products with Bioactive Potential for Tissue Engineering

Râpă¹,

Gaidău²,

Stefan³

et al. 2020

Prime Archives in Material Science

View full text Add to dashboard Cite

Concentrated collagen hydrolysate (HC10CC), rabbit collagen glue (RCG) and keratin hydrolysate (KH) were investigated in terms of their extraction from mammalian by-products and processing by electrospinning. The electrospun nanofibers were characterized by scanning electron microscopy coupled with the energy dispersive X-ray spectroscopy (SEM/EDS), attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FT-IR), differential scanning calorimetry (DSC), and indentation tests. The cytotoxicity of the electrospun nanofibers was conducted on L929 fibroblast cells using MTT and LDH assays and cell morphology observations. The electrospun RCG and KH nanofibers morphology showed an average size of nanofibers ranging between 44 and 410 nm, while the electrospun HC10CC nanofibers exhibited higher sizes. The ATR-FT-IR spectra performed both on extracted proteins and electrospun nanofibers showed that the triple helix structure of collagen is partially preserved. The results were in agreement with the circular dichroism analysis for protein extracts. Also, the viscoelastic properties of electrospun KH nanofibers were superior to those of electrospun RCG nanofibers. Based on both quantitative and qualitative analysis, the electrospun nanofibers were not cytotoxic, inducing a healthy cellular response. The results of new electrospun protein-based nanofibers may be useful for further research on bioactive properties of these nanofibers for tissue engineering.

show abstract

“…Total nitrogen and protein substances, dry substances, and total ash were measured according to ISO 5397, EN ISO 4684, and EN ISO 4047, respectively. Aminic nitrogen and average molecular weight (M w ) were evaluated by validated in-house methods [ 36 ]. Gel permeation chromatography (GPC) was accomplished using an Agilent Technologies instrument (1260 model) (Santa Clara, CA, USA) equipped with PL aquagel-OH MIXED-H column (7.5 × 300 mm, 8 μm) and multidetection unit (260 GPC/SEC MDS containing RID, LS, and VS detectors).…”

Section: Methodsmentioning

confidence: 99%

“…Collagen hydrolysate (HC10) is an accessible and inexpensive resource of collagen, which was not used in typical electrospinning processes due to its low molecular weight (3.95 kDa) and low viscosity (1.5 cP) in water ( Table 2). Concentrated collagen hydrolysate (HC10CC) has associative properties, i.e., its molecular weight increased by 4.5 times as compared to HC10 [36], and its viscosity increased to 615 cP (Table 2), which makes it an interesting material for electrospinning. The conductivity of KH shows the highest value due to the high content in salts, which influences the particle size and stability.…”

Section: Characterization Of Hc10 Hc10cc Rcg and Kh Extractsmentioning

confidence: 99%

See 1 more Smart Citation

New Nanofibers Based on Protein By-Products with Bioactive Potential for Tissue Engineering

et al. 2020

View full text Add to dashboard Cite

Concentrated collagen hydrolysate (HC10CC), rabbit collagen glue (RCG), and keratin hydrolysate (KH) were investigated in terms of their extraction from mammalian by-products and processing by electrospinning. The electrospun nanofibers were characterized by scanning electron microscopy coupled with the energy dispersive X-ray spectroscopy (SEM/EDS), attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), differential scanning calorimetry (DSC), and indentation tests. The cytotoxicity of the electrospun nanofibers was conducted on L929 fibroblast cells using MTT and LDH assays and cell morphology observations. The electrospun RCG and KH nanofibers morphology showed an average size of nanofibers ranging between 44 and 410 nm, while the electrospun HC10CC nanofibers exhibited higher sizes. The ATR-FTIR spectra performed both on extracted proteins and electrospun nanofibers showed that the triple helix structure of collagen is partially preserved. The results were in agreement with the circular dichroism analysis for protein extracts. Furthermore, the viscoelastic properties of electrospun KH nanofibers were superior to those of electrospun RCG nanofibers. Based on both in vitro quantitative and qualitative analysis, the electrospun nanofibers were not cytotoxic, inducing a healthy cellular response. The results of new electrospun protein-based nanofibers may be useful for further research on bioactive properties of these nanofibers for tissue engineering.

show abstract

Collagen Hydrolysate as Sustainable Additive for Cereal Seed Treatment

Cited by 4 publications

References 2 publications

Biostimulant Action of Protein Hydrolysates: Unraveling Their Effects on Plant Physiology and Microbiome

Biostimulant Action of Protein Hydrolysates: Unraveling Their Effects on Plant Physiology and Microbiome

New Nanofibers based on Protein By-Products with Bioactive Potential for Tissue Engineering

New Nanofibers Based on Protein By-Products with Bioactive Potential for Tissue Engineering

Contact Info

Product

Resources

About