Collagen I and III and metalloproteinase gene and protein expression in prostate cancer in relation to Gleason score

Duarte, Antonio H.; Colli, Sicilia; Alves-Pereira, Jorge Luiz; Martins, Max Peixoto; Sampaio, Francisco J. B.; Ramos, Cristiane F.

doi:10.1590/s1677-55382012000300007

Cited by 11 publications

(15 citation statements)

References 17 publications

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“…Still, we made an interesting observation that collagen III mRNA expression was significantly lower in tumor site derived cells. Decreased immunostaining for collagen III in prostate tumor when compared to benign areas has been reported . Furthermore, data in Proteinatlas/Pathology suggest very low expression levels of collagen III in prostate cancer.…”

Section: Discussionmentioning

confidence: 97%

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The composition of prostate core matrisome in vivo and in vitro unveiled by mass spectrometric analysis

et al. 2018

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Section: Discussionmentioning

confidence: 97%

“…Decreased immunostaining for collagen III in prostate tumor when compared to benign areas has been reported. 52 Furthermore, data in Proteinatlas/ Pathology 53 suggest very low expression levels of collagen III in prostate cancer. Thus our result may explain these previous in vivo observations.…”

Section: Matrisome In Spheroid-type Culture Differs From the Matrismentioning

confidence: 99%

The composition of prostate core matrisome in vivo and in vitro unveiled by mass spectrometric analysis

et al. 2018

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“…Coll-I and III gene and protein expression were previously evaluated in human PC, using RT-qPCR and IHC [37]. These authors also correlated Coll-I expression with Gleason score.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Collagen Fibers (I, III, IV) and Elastin of Normal and Neoplastic Canine Prostatic Tissues

Calderón

Kobayashi

Vasconcelos

et al. 2019

Veterinary Sciences

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This study aimed to investigate collagen (Coll-I, III, IV) and elastin in canine normal prostate and prostate cancer (PC) using Picrosirius red (PSR) and Immunohistochemical (IHC) analysis. Eight normal prostates and 10 PC from formalin-fixed, paraffin-embedded samples were used. Collagen fibers area was analyzed with ImageJ software. The distribution of Coll-I and Coll-III was approximately 80% around prostatic ducts and acini, 15% among smooth muscle, and 5% surrounding blood vessels, in both normal prostate and PC. There was a higher median area of Coll-III in PC when compared to normal prostatic tissue (p = 0.001 for PSR and p = 0.05 for IHC). Immunostaining for Coll-IV was observed in the basal membrane of prostate acini, smooth muscle, blood vessels, and nerve fibers of normal and PC samples. Although there was no difference in Coll-IV area between normal tissue and PC, tumors with Gleason score 10 showed absence of Coll-IV, when compared to scores 6 and 8 (p = 0.0095). Elastic fibers were found in the septa dividing the lobules and around the prostatic acini of normal samples and were statistically higher in PC compared to normal tissue (p = 0.00229). Investigation of ECM components brings new information and should be correlated with prognosis in future studies.

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“…In one study gene and protein expression of Col-I and Col-III were evaluated in human PC, in relation to Gleason score, using RT-qPCR and IHC [25]. No correlation was found between protein and gene expression for both collagens.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Collagens Fibers (I, III, IV) and Elastin in the Extracellular Matrix of Normal and Neoplastic Canine Prostate

Calderón¹,

Kobayashi²,

Vasconcelos³

et al. 2019

Preprint

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Collagen (Coll) is the most common protein in the extracellular matrix, responsible for providing tissue structure and support. In some types of cancer, including prostate cancer (PC) abundant collagen was identified and related to tumor progression and metastasis. This study aimed to investigate Coll-I, III, IV and elastin in normal canine prostatic tissue and PC, using the Picrosirius red (PSR) and Immunohistochemical (IHC) analysis. Eight normal prostates and 10 PC from formalin-fixed, paraffin-embedded samples were used. Collagen fibers area was analyzed with ImageJ software. The distribution of Coll-I and Col-III was approximately 80% around prostatic ducts and acini, 15% among smooth muscle and 5% around in blood vessels, in both normal prostate and PC. Immunostaining for type IV collagen was observed in the basal membrane of prostate acini, smooth muscle, blood vessels, and never fibers of normal and PC samples. Elastic fibers were found in the septa dividing the lobules and around the prostatic acini of normal samples. A high amount of elastic fibers was observed around the ducts and the urethra in normal and canine PC. The distribution and area percentage of staining for collagen are similar in normal and neoplastic canine prostate when analyzed with PSR and IHC.

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Collagen I and III and metalloproteinase gene and protein expression in prostate cancer in relation to Gleason score

Cited by 11 publications

References 17 publications

The composition of prostate core matrisome in vivo and in vitro unveiled by mass spectrometric analysis

The composition of prostate core matrisome in vivo and in vitro unveiled by mass spectrometric analysis

Characterization of Collagen Fibers (I, III, IV) and Elastin of Normal and Neoplastic Canine Prostatic Tissues

Characterization of Collagens Fibers (I, III, IV) and Elastin in the Extracellular Matrix of Normal and Neoplastic Canine Prostate

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