Collagen‐Induced Arthritis Mouse Model

Abstract

The collagen-induced arthritis mouse model is a widely studied autoimmune model of rheumatoid arthritis. In this model, autoimmune arthritis is induced by immunization of genetically susceptible strains of mice with type II collagen emulsified in complete Freund's adjuvant. This article describes the steps necessary for the acquisition, handling, and preparation of CII, in addition to the selection of mouse strains, proper immunization technique, and methods for evaluation of the incidence and severity of the autoimmune arthritis. In this model, the first signs of arthritis appear approximately 21 to 28 days after immunization. The protocols in this article should provide the investigator with all the necessary information required to reproducibly induce a high incidence of CIA in genetically susceptible strains of mice, and to critically evaluate the pathology of the disease.

Role of Citrullinated Collagen in Autoimmune Arthritis

“…Native type I collagen was solubilized from bovine skin and native type II collagen was solubilized from fetal calf articular cartilage by limited-pepsin digestion and purified as described earlier [ 11 ]. The purified collagen was dissolved in cold 10 mM acetic acid at 3 or 4 mg/mL and stored frozen at −70 °C until used.…”

“…In this study, we gained new insights into the mechanisms by which citrullination of collagen controls autoimmune arthritis. We used the well-studied mouse model of arthritis collagen-induced arthritis (CIA) because it mimics RA [ 11 ]. After the PADi4 enzyme was employed to citrullinate collagen, the resulting proteins were tested for binding and activation of LAIR-1.…”

Dual-specificity phosphatase 5 is a novel target gene of miR-216a-3p, inhibits the proliferation and invasion of fibroblast-like synoviocytes

“…Mice were then monitored every other day for arthritis progression. The severity of arthritis in all four paws of mice was evaluated using a ve-degree score to consider the different combinations of in amed groups of joints and to reduce the in uence of subjectivity on the investigator's evaluation [30,31].…”

“…55, 2001) and the guidelines for the Care and Use of Laboratory Animals of the Army Medical University (Chongqing, China). Male DBA/1 J mice (6-8 weeks old) were injected intradermally at the tail base with chicken type II collagen (CII) (Chondrex, Redmond, USA) as described previously [30]. On day 20, mice were given a booster immunization of 100 µL CII emulsi ed with Incomplete Freund's Adjuvant (IFA, Chondrex, Redmond, USA).…”

Advances in experimental models of rheumatoid arthritis

“…Researchers rely on animal models that span multiple species and strategic scientific approaches to tackle these objectives. Although researchers have attempted to establish RA models in various animals, including rodents [27], rabbits [28], minipigs [29, 30], and non‐human primates [31, 32] over the past few decades, no single model can replicate all aspects of human RA.…”