Collagen membrane functionalized with magnesium oxide via room-temperature atomic layer deposition promotes osteopromotive and antimicrobial properties

Saha, Saswata; Costa, Raphael Cavalcante; Silva, Mirela Caroline; Santos, João Matheus Fonseca e; Chen, Lin; Phakatkar, Abhijit H.; Bhatia, Harshdeep; Faverani, Leonardo Pérez; Barão, Valentim Adelino Ricardo; Shokuhfar, Tolou; Sukotjo, Cortino; Takoudis, Christos G.

doi:10.1016/j.bioactmat.2023.07.013

Cited by 5 publications

(4 citation statements)

References 106 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…28,29 However, the magnesium oxide scaffold investigated by Saha et al faces the challenge of increased magnesium ion release at higher temperatures. 30 Similarly, the titanium scaffold examined by Choi et al exhibits excellent biocompatibility and physical properties, but it may encounter difficulties with absorption when implanted in vivo. 31 Moreover, hydroxyapatite can promote the repair of damaged tissues.…”

Section: A Physical Characterization Of High-strength Ceramic Artific...mentioning

confidence: 99%

Preparation and in vitro osteogenic evaluation of high-strength ceramic artificial bone loaded with anti-tuberculosis drug PaMZ

Li,

Fei,

Zhang

et al. 2023

AIP Advances

View full text Add to dashboard Cite

The objective of this study was to prepare a high-strength ceramic artificial bone loaded with the anti-tuberculosis drug PaMZ (delamanid, moxifloxacin, and pyrazinamide) and evaluate its physical characteristics and osteogenic potential. We utilized 3D printing technology to fabricate artificial bones and then obtained a high-strength ceramic artificial bone by high-temperature firing. Then, a triple combination of anti-tuberculosis drugs, including delamanid (Pa), moxifloxacin (M), pyrazinamide (Z), and polylactic acid-co-glycolic acid mixed in a ratio of 3:12:45:140, was incorporated onto the surface of the ceramic artificial bone. Consequently, a high-strength ceramic artificial bone, loaded with anti-tuberculosis drugs, was successfully obtained. The physical characteristics of the drug-loaded artificial bone were assessed using an electronic universal testing machine and scanning electron microscopy. The osteogenic performance of the artificial bone was evaluated through rat bone marrow mesenchymal stem cell (rBMSCs) co-culture experiment, cell counting kit-8 (CCK-8) cell proliferation assay, alkaline phosphatase staining, and alizarin red staining. The drug-loaded ceramic artificial bone exhibited favorable physical characteristics, void interconnection, a porosity of 30.6% ± 0.7%, and a compressive strength of 17.65 ± 0.46 MPa. The rBMSCs co-culture experiment and CCK-8 cell proliferation experiment demonstrated excellent cell compatibility, while alkaline phosphatase and alizarin red staining indicated good in vitro osteogenic performance. In summary, the high-strength ceramic artificial bone loaded with the anti-tuberculosis drug PaMZ exhibited a favorable morphological structure and compressive strength. In addition, it demonstrated good biocompatibility and osteogenic properties.

show abstract

Section: A Physical Characterization Of High-strength Ceramic Artific...mentioning

confidence: 99%

Preparation and in vitro osteogenic evaluation of high-strength ceramic artificial bone loaded with anti-tuberculosis drug PaMZ

Li,

Fei,

Zhang

et al. 2023

AIP Advances

View full text Add to dashboard Cite

show abstract

“…In addition, porous substrates, 3D-printed scaffolds made of titanium [27,122] and iron [86], as well as the denture material PMMA [53,63,64], have been used. Of particular note is the study [133] where osteopromotive MgO coatings were applied to the surface of collagen, which is an integral part of bone, cartilage, skin, and tendon tissue. ZnO [26,43,44,80,86,87], TiO 2 [53,57,61], MgO [133], hydroxyapatite [98], ZrO 2 [96], TaN [28], Ag [27,57,122], and TiO 2 -ZrO 2 complex oxides [39,40] have been most commonly used as coatings [63,64].…”

Section: Medical Implantsmentioning

confidence: 99%

“…In the majority of cases for orthopedic implants, antibacterial properties were evaluated using S. aureus and E. coli, and, less frequently, MRSA [49,122] and S. epidermidis [122]. For dental implants, the set of strains included Porphyromonas gingivalis [80,87,96], S. Sanguinis, Bifidobacterium [80], S. oralis [63], C. albicans [53,63,64], and antibacterial polymicrobial biofilm from human saliva [133]. In addition to the antibacterial properties, in vitro biocompatibility studies were performed using osteoblast-like MC3T3-E1 [43,44,84,86,87,96,134], MG-63 [26,57], and SAOS-2 osteosarcoma [122], fibroblasts (L929) [26,98,134], and gingival keratinocytes as well as epithelial cells [80,81,122].…”

Section: Medical Implantsmentioning

confidence: 99%

“…In addition to the above, only a few studies have used the in vivo approach to evaluate the prospects of ALD coatings. The similar Wistar rat (Rattus norvegicus) in vivo model was used to confirm the biocompatibility of ALD MgO nanocoatings and to increase angiogenesis and bone formation [133]. The thicker coatings (500 vs. 200 cycles) were better candidates for guided bone regeneration due to the higher release of magnesium ions.…”

Section: Medical Implantsmentioning

confidence: 99%

See 1 more Smart Citation

Atomic Layer Deposition of Antibacterial Nanocoatings: A Review

Nazarov,

Kozlova,

Rogacheva

et al. 2023

Antibiotics

View full text Add to dashboard Cite

In recent years, antibacterial coatings have become an important approach in the global fight against bacterial pathogens. Developments in materials science, chemistry, and biochemistry have led to a plethora of materials and chemical compounds that have the potential to create antibacterial coatings. However, insufficient attention has been paid to the analysis of the techniques and technologies used to apply these coatings. Among the various inorganic coating techniques, atomic layer deposition (ALD) is worthy of note. It enables the successful synthesis of high-purity inorganic nanocoatings on surfaces of complex shape and topography, while also providing precise control over their thickness and composition. ALD has various industrial applications, but its practical application in medicine is still limited. In recent years, a considerable number of papers have been published on the proposed use of thin films and coatings produced via ALD in medicine, notably those with antibacterial properties. The aim of this paper is to carefully evaluate and analyze the relevant literature on this topic. Simple oxide coatings, including TiO2, ZnO, Fe2O3, MgO, and ZrO2, were examined, as well as coatings containing metal nanoparticles such as Ag, Cu, Pt, and Au, and mixed systems such as TiO2-ZnO, TiO2-ZrO2, ZnO-Al2O3, TiO2-Ag, and ZnO-Ag. Through comparative analysis, we have been able to draw conclusions on the effectiveness of various antibacterial coatings of different compositions, including key characteristics such as thickness, morphology, and crystal structure. The use of ALD in the development of antibacterial coatings for various applications was analyzed. Furthermore, assumptions were made about the most promising areas of development. The final section provides a comparison of different coatings, as well as the advantages, disadvantages, and prospects of using ALD for the industrial production of antibacterial coatings.

show abstract

Diffusion model assisted designing self-assembling collagen mimetic peptides as biocompatible materials

Wang,

Xu,

et al. 2024

Briefings in Bioinformatics

View full text Add to dashboard Cite

Collagen self-assembly supports its mechanical function, but controlling collagen mimetic peptides (CMPs) to self-assemble into higher-order oligomers with numerous functions remains challenging due to the vast potential amino acid sequence space. Herein, we developed a diffusion model to learn features from different types of human collagens and generate CMPs; obtaining 66% of synthetic CMPs could self-assemble into triple helices. Triple-helical and untwisting states were probed by melting temperature (Tm); hence, we developed a model to predict collagen Tm, achieving a state-of-art Pearson’s correlation (PC) of 0.95 by cross-validation and a PC of 0.8 for predicting Tm values of synthetic CMPs. Our chemically synthesized short CMPs and recombinantly expressed long CMPs could self-assemble, with the lowest requirement for hydrogel formation at a concentration of 0.08% (w/v). Five CMPs could promote osteoblast differentiation. Our results demonstrated the potential for using computer-aided methods to design functional self-assembling CMPs.

show abstract

Collagen membrane functionalized with magnesium oxide via room-temperature atomic layer deposition promotes osteopromotive and antimicrobial properties

Cited by 5 publications

References 106 publications

Preparation and in vitro osteogenic evaluation of high-strength ceramic artificial bone loaded with anti-tuberculosis drug PaMZ

Preparation and in vitro osteogenic evaluation of high-strength ceramic artificial bone loaded with anti-tuberculosis drug PaMZ

Atomic Layer Deposition of Antibacterial Nanocoatings: A Review

Diffusion model assisted designing self-assembling collagen mimetic peptides as biocompatible materials

Contact Info

Product

Resources

About