Collagen Nanofiber on Titanium or Partially Stabilized Zirconia by Electrospray Deposition

Abstract

The present study demonstrated collagen nanofiber deposition onto titanium and partially stabilized zirconia, yttria-stabilized zirconia (Y-TZP), using an electrospray deposition (ESD) technique. Type I collagen was dissolved into 1,1,1,2,2,2-hexafluoro-2-propanol at different concentrations. The effects of the flow rate of the collagen solution and the applied voltage on collagen nanofiber deposition were investigated.For titanium substrates, a collagen concentration of 50 mg/ml, a flow rate of 5.0 µl/min, and an applied voltage of 16 kV were found to be suitable for the deposition of uniform collagen nanofibers. Collagen nanofibers could be sprayed onto Y-TZP, which is not an electrically conductive substrate, although nanofibers fused during ESD. To avoid the fusion of nanofibers, titanium sputter-coating was performed on Y-TZP. Less fusion of collagen fibers occurred. A collagen concentration of 50 mg/ml, a flow rate of 5.0 µl/min, and an applied voltage of 25 kV were found to be appropriate for the deposition of uniform collagen nanofibers onto titanium sputter-coated Y-TZP. The ESD technique was shown to be effective for the deposition of collagen nanofibers.

Pilot study of gingival connective tissue responses to 3-dimensional collagen nanofiber-coated dental implants

“…Electrospun scaffolds consisting of polycaprolactone, collagen, and nanoparticulate hydroxyapatite were reported to support the adhesion, integrin-related signaling, and proliferation of mesenchymal stem cells, while the pore size of electrospun scaffolds influenced cell infiltration 23) . Hayakawa et al 24) confirmed the formation of collagen nanofibers on titanium disk surfaces by changing some conditions for ESD, such as the collagen concentration, flow rate and voltage applied.…”

“…In their study, the deposited ALP was a 2-dimensional film structure, not a 3-dimensional fiber structure. Our previous studies demonstrated that a collagen concentration of 50 mg/mL, flow rate of 5.0 mL/min, and applied voltage of 25 kV produced 3-dimensional uniform structures for collagen nanofibers with a diameter of approximately 200-280 nm 24) . Thus, the same conditions were used to deposit collagen nanofibers onto the screw-type titanium implants.…”