Collagen Patches Impregnated with Antimicrobial Agents Have High Local Antimicrobial Efficacy and Achieve Effective Tissue Gluing

Baar, S.; Schörner, Christoph; Röllinghoff, Martin; Radespiel‐Tröger, Martin; Hümmer, H. P.; Carbon, R.

doi:10.1007/s15010-001-0073-6

Cited by 10 publications

(3 citation statements)

References 29 publications

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“…By plasticity we understand an ability of material to change shape and sizes without being destroyed through exposure to external force. Plasticity is measured by such parameters as percentage elongation (contraction) δ and lateral contraction (expansion) ψ during a static test for stretching (contraction) [73][74][75]:…”

Section: Reviewsmentioning

confidence: 99%

In vitro Evaluation of Performance Properties of Sponge Hemostatic Dressings (Review)

Северинов¹,

Lazarenko²,

Sotnikov³

et al. 2020

Sovrem Tehnol Med

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Dressings for restoring organ defects and/or hemostasis in the injury site are being actively applied in operational units. These dressings are used in various surgeries and are widely represented in the foreign and domestic markets of medical products. Many local implants have different levels of hemostatic activity, which requires standardization of the algorithm of choice and the methods of their study. Here the methods of studying the performance properties of hemostatic implants in vitro have been considered and evaluation criteria of their physical, chemical and organoleptic properties in vitro have been proposed. This will allow a researcher to choose optimal variants of samples for further experiments on biological models more effectively as well as to save funds, time and reduce the number of experiments in vivo.

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Section: Reviewsmentioning

confidence: 99%

In vitro Evaluation of Performance Properties of Sponge Hemostatic Dressings (Review)

Северинов¹,

Lazarenko²,

Sotnikov³

et al. 2020

Sovrem Tehnol Med

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“…Therefore, a method for sustained controlled local delivery is desirable. Previously, CaSO 4 pellets have been shown to be a reliable, nontoxic delivery method for antibiotics in the treatment of osteomyelitis, 20 collagen sponges have been used to deliver antibiotics and growth factors, 21,22,23 and demineralized bone matrix has been used as a bone graft material. 24,25 These controlled-release systems are all materials that are readily available to orthopaedic surgeons in the operating room.…”

Section: Introductionmentioning

confidence: 99%

An evaluation of carrier agents for desferoxamine, an up-regulator of vascular endothelial growth factor

et al. 2012

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Avascularity and hypoxia result in avascular necrosis and play a negative role in fracture healing. The FDA-approved iron chelating agent, desferoxamine (DFO) in a liquid form, has been shown to induce angiogenesis and improve fracture healing through upregulation of the vascular endothelial growth factor. We were concerned that local injection of DFO would either fail to adequately deliver sufficient drug to the desired site or lead to undesired delivery to adjacent sites. Therefore, a sustained release delivery system was desirable to direct DFO to the intended site. Calcium sulfate pellets, collagen sponges, and demineralized cortical bone matrix were all evaluated as potentially controlled release systems for DFO using a fetal mouse metatarsal angiogenesis assay. Angiogenesis was analyzed using a vascularity grading scale, by measuring the mean vessel length of the 5 longest vessels, and by counting the mean number of vessels per metatarsal. Although there was some evidence of angiogenesis with all three carriers, DFO loaded CaSO4 pellets increased vascularity grading, the mean length of the five longest vessels, and the mean number of vessels, all by statistically significant margins versus the control. These results suggest that CaSO4 pellets could be used as a viable, nontoxic, controlled release system for DFO in clinical situations where increased angiogenesis and bone growth are desirable.

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“…Gentamicin‐loaded collagen fleece is used primarily in the surgical treatment of localized bone and soft tissues infections and in the prevention of wound infections 1–8…”

Section: Introductionmentioning

confidence: 99%

Elution kinetics, antimicrobial efficacy, and degradation and microvasculature of a new gentamicin‐loaded collagen fleece

et al. 2008

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Management of bone and soft tissue infections generally includes surgical procedures as well as attendant treatment and prevention with gentamicin-loaded fleeces. Conventional gentamicin-containing collagen fleeces currently in use are strongly acidic and exhibit limited biocompatibility thereby adversely affecting wound healing. To improve the antibiotic delivery system, a new phosphate-buffered, gentamicin-loaded fleece with pH-neutral properties has been developed (Jason G). This study aimed at comparing the elution kinetics of gentamicin release and the antimicrobial efficacy of conventional fleeces with the newly developed fleece in vitro. In addition, degradation and microvasculature of implanted fleeces were examined in a rat model and assessed using histology, as well as detection of ED-1 and PECAM-expression using immunohistochemistry. We show that the phosphate-buffered fleeces have reduced release (p < 0.05) of the integrated gentamicin. However, all of the fleeces tested had a significant antimicrobial effect on the growth of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa strains (p < 0.01). Among the fleeces tested, the new Jason G fleece had the weakest but nevertheless sufficient antimicrobial effectiveness. Evaluation of the antibiotic effect in the prevention of an infection showed no differences between the applied fleeces. Following surgical implantation of fleece in the backs of Wistar rats we observed, on day 5 after implantation, an increase in cell infiltration and microvascularization with the phosphate-buffered fleece as compared with conventional fleeces, which show necrotic cells on their surface. Unlike the acidic fleeces, on day 15 after implantation the pH-neutral fleece was resorbed widely. Here, we show that the new, pH-neutral, gentamicin-containing fleece Jason G exhibits good overall antimicrobial effectiveness against both gram-positive and gram-negative bacteria in vitro with improved degradation properties and microvasculature formation in vivo.

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Collagen Patches Impregnated with Antimicrobial Agents Have High Local Antimicrobial Efficacy and Achieve Effective Tissue Gluing

Abstract: Due to ease of practical handling and favorable pharmacoeconomics, this DDS is recommended for both open and minimally invasive surgery.

Cited by 10 publications

References 29 publications

In vitro Evaluation of Performance Properties of Sponge Hemostatic Dressings (Review)

In vitro Evaluation of Performance Properties of Sponge Hemostatic Dressings (Review)

An evaluation of carrier agents for desferoxamine, an up-regulator of vascular endothelial growth factor

Elution kinetics, antimicrobial efficacy, and degradation and microvasculature of a new gentamicin‐loaded collagen fleece

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