Collagen peptides modulate the metabolism of extracellular matrix by human dermal fibroblasts derived from sun‐protected and sun‐exposed body sites

Abstract

Clinical data published in recent years have demonstrated positive effects of collagen hydrolysate (CH) on skin aging clinical signs. CH use as food supplement has a long history; however, few studies have addressed the underlying purpose of CH on the cellular and molecular biology of skin cells that could elucidate clinical improvement findings. Wide diversity of characteristics has been reported for dermal fibroblasts derived from different body sites and it is unknown whether collagen peptides could modulate differently cells from chronological aged and photoaged skin areas. This study investigated the influence of CH on the extracellular matrix metabolism and proliferation of human dermal fibroblasts (HDFs) derived from chronological aged (sun-protected) and photoaged (sun-exposed) body sites. CH treatment did not affect cellular proliferation of either cell cultures, but notably modulated cell metabolism in monolayer model, increasing the content of dermal matrix precursor and main protein, procollagen I and collagen I, respectively. These effects were confirmed in the human dermal equivalent model. The increase in collagen content in the cultures was attributed to stimulation of biosynthesis and decreased collagen I metabolism through inhibition of metalloproteinase activity (MMP) 1 and 2. Modulation of CH in dermal metabolism did not differ between cells derived from sun-protected and sun-exposed areas, although lower concentrations of CH seemed to be enough to stimulate sun-exposed-derived HDFs, suggesting more pronounced effect in these cells. This study contributes to understanding the biological effects of CH on skin cells and viability of its use as a functional ingredient in food supplements.

Enhancing Skin Health: By Oral Administration of Natural Compounds and Minerals with Implications to the Dermal Microbiome

“…In 2018, Zague et al reported that collagen peptides modulate the metabolism of extracellular matrix proteins by human dermal fibroblasts (in culture) that were derived from sun-protected and sun-exposed body sites [ 23 ]. The in vitro collagen hydrolysate treatment increased the dermal matrix precursors along with procollagen type I and collagen type I proteins.…”

“…The in vitro collagen hydrolysate treatment increased the dermal matrix precursors along with procollagen type I and collagen type I proteins. The increased levels of collagen were attributed to enhanced biosynthesis of collagens by fibroblasts but also decreased collagen type I metabolism through the inhibition of metalloproteinases (MMP 1 and MMP 2) activities [ 23 ]. The authors concluded that food-derived collagen hydrolysates improved skin cells and dermal health by enhancing collagen production and inhibiting the enzymes that break it down.…”

Effects of a Combination of Water-Soluble Coenzyme Q10 and Collagen on Skin Parameters and Condition: Results of a Randomised, Placebo-Controlled, Double-Blind Study

“…While in our previous study there were no significant effects on dermal density determined after 12 weeks of supplementation with CoQ10 alone, by the addition of collagen peptides and other nutrients to the test product, significant improvement in dermal density (16.1%) in comparison to the placebo was achieved in the present study, while dermis thickness was not affected. As dermis density is related to the amount of properly structured dermal proteins, e.g., collagen and elastin, the test product may have reduced degradation or promoted the synthesis of structural proteins as shown for collagen peptides in some in vivo [15], animal [45] and in vitro [46] studies as well in some in vitro studies for CoQ10 [39,42,43]. The observed improvement in dermal density is almost double when compared to the previously reported influence of supplementation, with a 2.5-fold dose of CP alone (10 g/day; 8.8% improvement) [17], indicating that some synergy may develop when using CoQ10 and CP simultaneously.…”

Alterations in cellular force parameters and cell projections in Nasal polyps-derived fibroblasts

“…Culture flasks were incubated at 37 C in a humidified atmosphere of 5% CO 2 . The second to third passages of fibroblasts were used for the experiment [34].…”