Collagen self-assembly and the development of tendon mechanical properties

Silver, Frederick H.; Freeman, Joseph W.; Seehra, Gurinder P.

doi:10.1016/s0021-9290(03)00135-0

Cited by 576 publications

(478 citation statements)

References 115 publications

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“…33,34 Sliding between fibrils occurs as mechanical loading is applied to tendons. 35,36 Patients with CACP syndrome, in which the lubricin/PRG 4 gene is defective, 13 significantly lose tendon elasticity. 37 We observed lubricin at the interface of collagen bundles where it may lower the adhesion between collagen bundles or perhaps serve another function related to tendon elasticity.…”

Section: Discussionmentioning

confidence: 99%

Expression and mapping of lubricin in canine flexor tendon

et al. 2006

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Matrix composition and the biomechanical environment are intimately interdependent in most connective tissues. Lubricin has many distinct biological functions, including lubrication, antiadhesion, and cytoprotection in cartilage, tendons, and other tissues. This study investigated the distribution of lubricin in the canine flexor digitorum profundus (FDP) tendon by immunohistochemistry. Lubricin was found both on the tendon surface and at the interface of collagen fiber bundles within the tendon, where the cells are subjected to shear force in addition to tension and compression. The expression of lubricin in regions of the canine flexor tendon that differ in mechanical or nutritional environment was also investigated using RT-PCR. Six N-terminal splicing variants were identified from six distinct anatomical regions of flexor tendon. The variants with larger sizes were noted in regions subjected to significant shear and compressive forces. Lubricin is ubiquitous in the FDP tendon, with variations in distribution and splicing that appear to correspond to discrete anatomic locations that differ by mechanical or nutritional environment. ß

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Section: Discussionmentioning

confidence: 99%

Expression and mapping of lubricin in canine flexor tendon

et al. 2006

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“…As discussed in the previous section, several authors have studied the effects of parameters like pH, ionic strength and temperature on fibrillogenesis [11,[22][23][24]37]. Some of them already pointed out that, regarding the process of self-assembly, the modulation of these parameters can determine modifications at the micro-structural level in terms of the fibril size and the electrostatic interactions between neighboring fibrils [20,21,23,[33][34][35]38].…”

Section: (Kpa)mentioning

confidence: 99%

“…This process results in the decomposition of collagen fibers and fibrils into small aggregates of collagen monomers dispersed in the acid solution. It has been widely documented that these small aggregates undergo in vitro -self-assembly‖ at neutral pH, in salt solution and at 37 °C or room temperature, forming fibrils with the typical banding pattern [11]. These gels show good biological properties and can be formed into tubular structures or into any shape suitable for further characterization [7,12,13].…”

Section: Introductionmentioning

confidence: 99%

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Tailoring Mechanical Properties of Collagen-Based Scaffolds for Vascular Tissue Engineering: The Effects of pH, Temperature and Ionic Strength on Gelation

2010

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Collagen gels have been widely studied for applications in tissue engineering because of their biological implications. Considering their use as scaffolds for vascular tissue engineering, the main limitation has always been related to their low mechanical properties. During the process of in vitro self-assembly, which leads to collagen gelation, the size of the fibrils, their chemical interactions, as well as the resulting microstructure are regulated by three main experimental conditions: pH, ionic strength and temperature. In this work, these three parameters were modulated in order to increase the mechanical properties of collagen gels. The effects on the gelation process were assessed by turbidimetric and scanning electron microscopy analyses. Turbidity measurements showed that gelation was affected by all three factors and scanning electron images confirmed that major changes occurred at the microstructural level. Mechanical tests showed that the compressive and tensile moduli increased by four-and three-fold, respectively, compared to the control. Finally, viability tests confirmed that these gels are suitable as scaffolds for cellular adhesion and proliferation.

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“…Silver et al 2003). The local mechanical response of these material structures is typically determined by elastin and collagen fibres, respectively cable-like bundles thereof.…”

Section: Introductionmentioning

confidence: 99%

A microsphere-based remodelling formulation for anisotropic biological tissues

Menzel

Waffenschmidt

2009

Phil. Trans. R. Soc. A.

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Biological tissues possess the ability to adapt according to the respective local loading conditions, which results in growth and remodelling phenomena. The main goal of this work is the development of a new remodelling approach that, on the one hand, reflects the alignment of fibrous soft biological tissue with respect to representative loading directions. On the other hand, the continuum approach proposed is based on a sound micro-mechanically motivated formulation. To be specific, use of a worm-like chain model is made to describe the behaviour of long-chain molecules as present in, for instance, collageneous tissues. The extension of such a one-dimensional constitutive equation to the three-dimensional macroscopic level is performed by means of a microsphere formulation. Inherent with the algorithmic treatment of this type of modelling approach, a finite number of unit vectors is considered for the numerical integration over the domain of the unit sphere. As a key aspect of this contribution, remodelling is incorporated by setting up evolution equations for the referential orientations of these integration directions. Accordingly, the unit vectors considered now allow interpretation as internal variables, which characterize the material's anisotropic properties. Several numerical studies underline the applicability of the model that, moreover, nicely fits into iterative finite element formulations so that general boundary value problems can be solved.

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Collagen self-assembly and the development of tendon mechanical properties

Cited by 576 publications

References 115 publications

Expression and mapping of lubricin in canine flexor tendon

Expression and mapping of lubricin in canine flexor tendon

Tailoring Mechanical Properties of Collagen-Based Scaffolds for Vascular Tissue Engineering: The Effects of pH, Temperature and Ionic Strength on Gelation

A microsphere-based remodelling formulation for anisotropic biological tissues

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