Collagen Synthesized in Fluorocarbon Polymer Implant in the Rabbit Cornea

Drubaix, Isabelle; Legeais, Jean‐Marc; Malek-Chehire, N.; Savoldelli, Michéle; Ménasche, Maurice; Robert, L.; Renard, G; Pouliquen, Y.

doi:10.1006/exer.1996.0042

Cited by 26 publications

(16 citation statements)

References 26 publications

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“…In contrast, Type III collagen was barely noticeable after 3 months, confirming the results obtained biochemically. 21 This result agrees with those of Girard et al and Hassel et al, who demonstrated that the rabbit cornea can regenerate portions of itself. 22,23 The dry weight returns to normal levels in 4 to 6 weeks.…”

Section: Discussionsupporting

confidence: 93%

“…12 SDS-PAGE and densitometric analysis revealed similar patterns of collagen-type distribution in the HP implant and the control stroma. 21 Immunogold labeling techniques confirmed these results and clearly identified Type I collagen to be the collagen predominantly synthesized in the polymer. In contrast, Type III collagen was barely noticeable after 3 months, confirming the results obtained biochemically.…”

Section: Discussionsupporting

confidence: 53%

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Influence of ePTFE polymer implant permeability on the rate and density of corneal extracellular matrix synthesis

Legeais¹,

Drubaix

Briat

et al. 1997

J. Biomed. Mater. Res.

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Microporous polymers have great potential for the production of corneal keratoprosthetic devices. Keratocytes invade the pores of expanded polytetrafluoroethylene implants (ePTFE) and collagen synthesis occurs. This ePTFE becomes translucent after its implantation in the stroma of rabbit cornea. The rate and density of cell growth within this polymer depends on the implant thickness, pore size, and its placement in the cornea. We have investigated the influence of the polymer permeability on the collagen and protein contents ePTFE implants. Rabbit corneal stroma were implanted with ePTFE disks (6 mm in diameter) by intralamellar keratoplasty. The implanted polymers were removed from the stroma after 3 to 6 months. The collagen and protein contents were determined after pepsin solubilization. The collagen content of the high-permeability implant was 3.7-fold greater than that of the low-permeability implant 3 months after implantation and 2.4-fold greater after 6 months. The total protein content of the high-permeability implant was 2.5-fold greater than that of low-permeability implant at 3 months and was the same after 6 months. The collagen-to-protein ratio was 68% in the high-permeability implants, and thus similar to that of normal corneal stroma. Thus, high polymer permeability increased both the rate and density of the corneal extracellular matrix ingrowth.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 53%

Influence of ePTFE polymer implant permeability on the rate and density of corneal extracellular matrix synthesis

Legeais¹,

Drubaix

Briat

et al. 1997

J. Biomed. Mater. Res.

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“…Normal corneal epithelial surfacing is also important for the success of artificial corneal implants including keratoprosthetics and epikeratophakia lenticules. The major complications leading to failure of cornea prosthetics developed to date include leakage, infection, epithelial down growth around the prosthetic, tissue necrosis and eventually extrusion of the implant [Cardona, 1991;Trinkaus-Randall et al, 1991;Girard, 1993;Drubaix et al, 1996;Vijayasekaran et al, 1997]. Providing a surface that would support a normal, stable, self-renewing epithelial layer still remains a challenge and is the key to designing a permanent keratoprosthetic or epikeratophakia lenticule.…”

Section: Discussionmentioning

confidence: 99%

Electron Microscopy of the Canine Corneal Basement Membranes

Abrams

Bentley

Nealey

et al. 2002

Cells Tissues Organs

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The purpose of this study was to characterize the surface topographical features of the epithelial and endothelial (Descemet’s) basement membranes of the canine cornea. Corneas were obtained from young, healthy dogs (<2 years old) with no history or evidence of previous ocular disease. The epithelium and endothelium was carefully removed preserving the anterior and posterior basement membranes. The specimens were examined by transmission electron microscopy and scanning electron microscopy. The epithelial and endothelial basement membrane surface topography is an intricate meshwork of pores and fibers measuring in the nanometer size range. The features of the endothelial basement membrane overall are smaller in size than the epithelial basement membrane. These surface topographical features may incite changes in epithelial and endothelial cell behavior.

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“…[4][5][6] This PTFE polymer is white opaque and hydrophobic before it is implanted and becomes translucent and wettable during its initial 15 days in situ. 4,5,7 It remains translucent for over a year. But this PTFE polymer occasionally fails to become translucent and wettable, or the translucence decreases over time, leading to an opaque polymer.…”

Section: Introductionmentioning

confidence: 99%

“…Keratocytes invade this PTFE polymer and rapidly adopt a corneal phenotype that is distinct from the dermal or scarring phenotype, as shown by the collagen types produced in the implant. 7 The behavior of the same PTFE polymer implanted in the anterior chamber of the rabbit eye and sutured to the posterior surface of the cornea also has been investigated. 8 The polymer is well tolerated and becomes translucent after only 8 days as compared to 12-15 days for intrastromal implantation.…”

Section: Introductionmentioning

confidence: 99%

Quantification and localization of hyaluronan in a PTFE polymer implanted in the corneal stroma

Drubaix

Legeais

Mounier³

et al. 1998

J. Biomed. Mater. Res.

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The amount and distribution of hyaluronan in a PTFE polymer used to support an artificial cornea implanted in the rabbit cornea were determined. The findings were used to describe the polymer-corneal stroma interface and the reason for the translucence and wettability of this originally opaque and hydrophobic biomaterial. PTFE disks (6 mm in diameter, 0.2 mm thick, 50 microns in pore size) were implanted after a free-hand intralamellar dissection. The corneas were removed 15 days, 1 month, and 3 months after implantation. The hyaluronan content of pepsin-solubilized corneal stromal extracts and its distribution (7 microns cryostat sections) were investigated using an alkaline phosphatase-linked hyaluronectin assay that specifically detects nanogram amounts of hyaluronan. A PTFE polymer implant caused large, transient increases in hyaluronan density in the implanted stroma. The presence of amphiphilic hyaluronan in the polymer 15 days post implantation probably produced translucence and wettability of this opaque, hydrophobic implant despite the absence of cells. The hyaluronan density in the PTFE polymer increased considerably during the first month and then decreased to stabilize at a moderate level by the third month. These changes in hyaluronan density parallel the invasion of the polymer by inflammatory cells during the first month and the subsequent replacement of these cells by fibroblasts. The PTFE polymer is a good interface that is compatible with the native corneal stroma, and our results indicate that hyaluronan, because of its amphiphilic character, plays a major role in the polymer wettability and translucence and in the production of typical corneal extracellular matrix within the pores of the polymer.

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Collagen Synthesized in Fluorocarbon Polymer Implant in the Rabbit Cornea

Cited by 26 publications

References 26 publications

Influence of ePTFE polymer implant permeability on the rate and density of corneal extracellular matrix synthesis

Influence of ePTFE polymer implant permeability on the rate and density of corneal extracellular matrix synthesis

Electron Microscopy of the Canine Corneal Basement Membranes

Quantification and localization of hyaluronan in a PTFE polymer implanted in the corneal stroma

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