Collagen types in keratoconus

Radda, T. M.; Menzel, E. J.; Freyler, H; Gnad, H. D.

doi:10.1007/bf02175894

Cited by 16 publications

(9 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Radda et al have not shown expression changes in type V collagen in KC cornea 38 ; however, our sequencing and validation data shows upregulation of COL5A3 in the KC-affected cornea. These contradictory results could be due to the differences in corneal sample processing (collagen types were separated using salt fractionation and thermal gelation in the pepsin-soluble fraction of the lyophilized normal and KC human corneal tissues) 38 .…”

Section: Discussioncontrasting

confidence: 59%

Differential Expression of Coding and Long Noncoding RNAs in Keratoconus-affected Corneas

Khaled

Bykhovskaya

Yablonski

et al. 2018

Preprint

View full text Add to dashboard Cite

PURPOSE Keratoconus (KC) is the most common corneal ectasia. We aimed to determine the differential expression of coding and long noncoding RNAs (lncRNAs) in human corneas affected with KC.METHODS 200ng total RNA from the corneas of 10 KC patients and 8 non-KC normal controls was used to prepare sequencing libraries with the SMARTer Stranded RNA-Seq kit after ribosomal RNA depletion. Paired-end 50bp sequences were generated using Illumina HiSeq 2500Sequencer. Differential analysis was done using TopHat and Cufflinks with a gene file from Ensembl and a lncRNA file from NONCODE. Pathway analysis was performed using WebGestalt.Using the expression level of differentially expressed coding and noncoding RNAs in each sample, we correlated their expression levels in KC and controls separately and identified significantly different correlations in KC against controls followed by visualization using Cytoscape. RESULTSUsing |fold change| ≥ 2 and a false discovery rate ≤ 0.05, we identified 436 coding RNAs and 584 lncRNAs with differential expression in the KC-affected corneas. Pathway analysis indicated the enrichment of genes involved in extracellular matrix, protein binding, glycosaminoglycan binding, and cell migration. Our correlation analysis identified 296 pairs of significant KC-specific correlations containing 117 coding genes enriched in functions related with cell migration/motility, extracellular space, cytokine response, and cell adhesion, suggesting the potential functions of these correlated lncRNAs, especially those with multiple pairs of correlations.CONCLUSIONS Our RNA-Seq based differential expression and correlation analyses have identified many potential KC contributing coding and noncoding RNAs.

show abstract

Section: Discussioncontrasting

confidence: 59%

Differential Expression of Coding and Long Noncoding RNAs in Keratoconus-affected Corneas

Khaled

Bykhovskaya

Yablonski

et al. 2018

Preprint

View full text Add to dashboard Cite

show abstract

“…Radda et al (1982) found a 5 % increase in type I collagen in keratoconus, while Zimmermann et al (1988) found no differences in collagen composition of biochemical extracts from keratoconus. Analysis of expression of collagens by keratocytes in cell culture further clouds the issue: Newsome et al (1981) stated that collagen types I and III were synthesized in similar amounts by both normal and keratoconic derived stromal cells, while Peters et al, (1993) analysed four cultures of keratoconic cells and found that type I collagen and steady-state RNAs were reduced in cells from three of the four cultures.…”

Section: Extracellular Matrix Proteinsmentioning

confidence: 89%

Cellular Incursion into Bowman's Membrane in the Peripheral Cone of the Keratoconic Cornea

Sherwin

Brookes

Loh

et al. 2002

Experimental Eye Research

View full text Add to dashboard Cite

“…The distribution of collagen types [1,2] as well as the posttranslational modification [2] of collagens were found to be within normal ranges. However, increased collagenolytic activities have been detected in keratoconus [3][4][5][6] and a suppressed translation of keratocyte RNA that could affect the synthesis of proteins like collagens has also been found [7,8].…”

Section: Introductionmentioning

confidence: 94%

Altered Organization of Collagen in the Apex of Keratoconus Corneas

Radner¹,

Zehetmayer²,

Skorpik³

et al. 1998

Ophthalmic Res

View full text Add to dashboard Cite

Purpose: In 15 keratoconus corneas, the three-dimensional arrangement of collagen lamellae was investigated by means of scanning electron microscopy. Methods: Keratoconus corneas without visible scars were obtained during perforating keratoplasty. The noncollagenous matrix of the stroma was removed with sodium hydroxide. Descemet’s membrane was removed mechanically and deeper layers of the stroma were exposed by cutting the tissue tangentially to the corneal surface with an ultramicrotome. The apical and the para-apical regions of keratoconus were compared to the central regions of normal corneas. Results: In the apical regions of 11 out of the 15 keratoconus corneas (73%), the arrangement of the collagen lamellae differs from those of the para-apical regions and normal corneas. Their collagen fibrils form uniform layers and no delimited collagen lamellae can be differentiated. Interlacing between adjacent layers is extremely decreased or even absent. In the para-apical region of keratoconus corneas the three-dimensional arrangement of collagen lamellae does not differ from that in normal corneas. Conclusion: Stromal thinning and conical ectasia in the apex of keratoconus corneas alters the organization of collagen. This will certainly affect the biomechanical properties of the cornea and further lead to a progression of keratoconus irrespective of its primary pathogenesis.

show abstract

Collagen types in keratoconus

Cited by 16 publications

References 11 publications

Differential Expression of Coding and Long Noncoding RNAs in Keratoconus-affected Corneas

Differential Expression of Coding and Long Noncoding RNAs in Keratoconus-affected Corneas

Cellular Incursion into Bowman's Membrane in the Peripheral Cone of the Keratoconic Cornea

Altered Organization of Collagen in the Apex of Keratoconus Corneas

Contact Info

Product

Resources

About