Collagenase activity in rabbit carcinoma: Cell source and cell interactions

Dabbous, Mustafa Kh.; Eltorky, Mahmoud; Haney, Lena; Brinkley, Sr. Burchada; Sobhy, Nahed

doi:10.1002/ijc.2910310317

Cited by 36 publications

(20 citation statements)

References 22 publications

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“…Many studies have examined the role of collagenolytic enzymes in tumour invasion and metastatic spread (for reviews see Woolley et al, 1980;Liotta et al, 1982; and recently several investigations have emphasised the importance of tumour:host cell interactions in connective tissue degradation (Tarin, 1976;Dabbous et al, 1977Dabbous et al, ,1983aBiswas, 1982;Henry et al, 1983). The tumour: host interface or 'invasion zone' (Strauli, 1980) of many invasive tumours is variable with regard to the type and relative numbers of host cells.…”

Section: Discussionmentioning

confidence: 99%

“…Collagenase was assayed by measuring the release of soluble radioactive peptides from 30 pl 14C-glycine-labelled reconstituted gel of collagen fibrils (Dabbous et al, 1983a) after 16h at 350C. One unit of collagenase degrades 1 jg collagen min 1 at 35°C and data is expressed as cumulative units per mg protein per 3 days of culture (Dabbous et al, 1983b).…”

Section: Histologymentioning

confidence: 99%

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Mast cells and matrix degradation at sites of tumour invasion in rat mammary adenocarcinoma

Dabbous¹,

Walker²,

Haney³

et al. 1986

Br J Cancer

111

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Summary Significant numbers of mast cells have been demonstrated histologically around the periphery of the invasive rat mammary adenocarcinoma 13672NF. The number of mast cells at microfoci along the tumour:host tissue junction was significantly greater than that found in normal mammary tissues, and few mast cells were detected within the tumour itself. Mast cell degranulation, often associated with disruption and lysis of the connective tissue matrix, was a common feature in later stages of tumour proliferation. When soluble products derived from purified rat peritoneal mast cells were added to monolayer cultures of rat stromal fibroblasts or tumour cells they stimulated a significant increase in total collagenase production, and the mast cell products were also capable of activating the latent collagenases thus produced. Histological examination indicated that degradation of local collagenous matrix was a common feature of mast cell degranulation, an observation possibly explained by the release of mast cell enzymes and/or the potential of this cell to modulate the expression of collagenolytic activity by surrounding cells. These observations suggest that, at least in some tumours, mast cells contribute to the connective tissue breakdown commonly associated with tumour invasiveness and metastatic spread.

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Section: Discussionmentioning

confidence: 99%

Section: Histologymentioning

confidence: 99%

Mast cells and matrix degradation at sites of tumour invasion in rat mammary adenocarcinoma

Dabbous¹,

Walker²,

Haney³

et al. 1986

Br J Cancer

111

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“…Rabbit VX2 carcinoma cells donated by Hanoch Alpern-Elran of this institute were a line grown in DMEM with 10% FBS from primary cultures of an enzyme-digested VX2 tumor. 51 These cells were seeded 1 week before experiments. All cell cultures were maintained at 37°C in an atmosphere of 95% air/5% CO 2 with a relative humidity of 95%.…”

Section: Cell Culturementioning

confidence: 99%

Interaction of native and cell-modified low density lipoprotein with collagen gel.

1988

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“…In fact, two subpopulations, each lacking a necessary but different enzyme, might be complementary so that a mixture of the two 'nonmetastatic' cells would metastasize. In some cases, the enzyme production itself may be the result of cellular interactions [91][92][93][94][95][96][97][98]. Lygns et al [91] found that two variants of a rat mammary carcinoma were interdependent.…”

Section: Invasion and Intravasationmentioning

confidence: 99%

Cellular interactions in metastasis

Miller¹,

Heppner²

1990

Cancer Metast Rev

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The metastatic cascade is a sequence of events that must be completed for metastases to be established. The realization that tumors are heterogeneous, consisting of many different subpopulations differing in many characteristics, and the belief that there are selective events in the metastatic process have led several laboratories to isolate and characterize variants with both high and low metastatic potential. Typically, the highly metastatic variants have been able to form distant metastases when implanted into the subcutis. Such lines have been popular for studies of metastatic mechanisms and anti-metastatic therapy, but they may be atypical examples, and thus not the best experimental models. Recent studies indicate that normal tissue influences metastasis such that many tumors metastasize only if placed in the orthotopic site. Furthermore, some cells that do not metastasize individually are able to do so in conjunction with other variant subpopulations. Thus, mixtures of tumor cells in the tissue of origin can express a more malignant character. We review possible mechanisms for such influential interactions, as well as the role of cellular interactions in generating heterogeneity and stabilizing tumor characteristics.

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Collagenase activity in rabbit carcinoma: Cell source and cell interactions

Cited by 36 publications

References 22 publications

Mast cells and matrix degradation at sites of tumour invasion in rat mammary adenocarcinoma

Mast cells and matrix degradation at sites of tumour invasion in rat mammary adenocarcinoma

Interaction of native and cell-modified low density lipoprotein with collagen gel.

Cellular interactions in metastasis

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