Collagenase and Sodium Iodoacetate-Induced Experimental Osteoarthritis Model in Sprague Dawley Rats

Al-Saffar, F. J.; Ganabadi, S.; Yaakub, Halimatun; Fakurazi, Sharida

doi:10.3923/ajsr.2009.167.179

Cited by 25 publications

(18 citation statements)

References 26 publications

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“…Group I were injected with 50 μl of normal saline solution and served as control. Animals from the second group were intra-articularly injected with 50 μl collagenase type II (Sigma Aldrich, USA) dissolved in saline (50 unit) into the right knee joint [9, 18]. The injection was performed on 1st and 4th day of the experiment [19].…”

Section: Methodsmentioning

confidence: 99%

New herbal composition (OA-F2) protects cartilage degeneration in a rat model of collagenase induced osteoarthritis

Nirmal

Jagtap

Narkhede

et al. 2017

BMC Complement Altern Med

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BackgroundPrevalence of osteoarthritis (OA) is on rise on the global scale. At present there are no satisfactory pharmacological agents for treating OA. Our previous study showed that Sida cordifolia L. and Zingiber officinale Rosc. had protective effect on cartilage. Here, we describe the effect of OA-F2, a herbal formulation prepared using combination of these two plants in alleviating OA associated symptoms in a rat model of collagenase-induced OA.MethodsOA was induced by intra-articular injection of collagenase type II in wistar rats. Diclofenac (10 mg/kg) was used as a reference control. Rats (n = 6) were divided into 6 groups: Healthy control (HC), osteoarthritic control (OAC), diclofenac (DICLO), OA-F2L (135 mg/kg), OA-F2M (270 mg/kg) and OA-F2H (540 mg/kg). The effects of the 20 days treatment were monitored by parameters like knee diameter, paw volume, paw retraction; serum C-reactive protein (CRP), alkaline phosphatase (ALP) and glycosaminoglycan (GAG). Radiography and histopathology of knee joint were also studied. Additionally, gene expression was studied from isolated synovium tissue proving anti-osteoarthritic potential of OA-F2.ResultsOral administration of OA-F2 has significantly prevented knee swelling compared to OAC; OA-F2 and DICLO, significantly reduced paw volume compared to OAC. Paw latency was remarkably increased by OA-F2 compared to OAC. OA-F2L (−0.670, p < 0.001), M (−0.110, p < 0.05) and H (0.073) has markedly reduced levels of CRP compared to DICLO. OA-F2L (p < 0.05), M (p < 0.001) and H (p < 0.05) significantly reduced ALP levels, compared to DICLO. GAG release in the serum was also significantly lowered in OA-F2 treated group compared to DICLO. Radiological and histopathological observations showed cartilage protection by OA-F2. OA-F2 has upregulated SOD and GPx. Upregulated CAT expression was observed in OA-F2M and H. Considerable down-regulation of expression of MMP-3 and MMP-9 was observed in all the groups. Up-regulation of TIMP-1 was observed in rats treated with OA-F2L, H and DICLO.ConclusionOA-F2 has shown therapeutic effects in rat model of collagenase induced OA by demonstrating cartilage protection through controlling MMPs and improving anti-oxidant levels in arthritic synovium and is a potent candidate for further drug development and treatment for OA.

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Section: Methodsmentioning

confidence: 99%

New herbal composition (OA-F2) protects cartilage degeneration in a rat model of collagenase induced osteoarthritis

Nirmal

Jagtap

Narkhede

et al. 2017

BMC Complement Altern Med

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“…The difference of swelling between both the knees was documented for all the groups of MIA-injected rats using a calibrated digital caliper (WORKZONE, China). 41 Radio imaging. Lateral views of the knee joint were taken at the 7th day under anaesthesia (5% Na-thiopentone) to appraise the outcome of the treatment on the experimental arthritic knee joint.…”

Section: Skin Irritation Studymentioning

confidence: 99%

“…Macroscopic investigation was performed to find the deformities of the joint capsules. 41,42 Tissue preparations for histopathology…”

Section: Skin Irritation Studymentioning

confidence: 99%

“…Decalcification time is directly proportional to the age of the animals. 41,43 The joints were embedded in paraffin and cut into frontal sections of femorotibial joints for conventional histopathology study under a light microscope. 17 Each section was stained with haematoxylin and eosin (H&E) to assess the pathological changes in the knee joints.…”

Section: Skin Irritation Studymentioning

confidence: 99%

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Accentuated transdermal application of glucosamine sulphate attenuates experimental osteoarthritis induced by monosodium iodoacetate

et al. 2016

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Osteoarthritis is a chronic degenerative joint disease causing pain and disability. Glucosamine sulphate is a well known oral supplement for its treatment. The present pioneering study provides an overview of the accentuated transdermal delivery of glucosamine sulphate through the optimized gel formulation with guar gum and sodium carboxymethyl cellulose (Na-CMC). Response surface methodology based on the three-level three-factor central composite design provided the optimum concentration of guar gum, Na-CMC and glycerol for a maximum flux. The transdermal characterization, ex vivo permeation study and in vivo study were performed with optimized gel formulation. The factorial design predicted the optimum values of guar gum, Na-CMC and glycerol which were 418.53 mg, 444.97 mg and 2322.4 mg respectively for 25 g of the gel. This optimized gel demonstrated the maximum flux,i.e., 1047.46 mg cm À2 h À1 . The optimized gel showed satisfactory results with respect to drug uniformity, pH, stability, rheological properties, zeta potential, drug-excipient compatibility and skin irritation.The release of the drug from the optimized transdermal gel followed the controlled first order Fickian (non-steady) release pattern. The in vivo study was carried out in a rat model of osteoarthritis induced by monosodium iodoacetate damaging the tibial plateau. In this study the optimized formulation effectively reduced the symptoms like reduction in swelling of the knee joint, gross changes in digitized radio images and morphological and histopathological alterations. Additionally the changes in the release pattern of the proinflammatory cytokine tumor necrosis factor-a illustrated the efficacy of the transdermal gel for the treatment of experimental osteoarthritis. Thus the optimized gel was found to be a unique potential vehicle for transdermal application of glucosamine sulphate which effectively attenuates the experimental osteoarthritis.

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“…The rats were individually caged in air-conditioned animal room at 22°C and were given commercial pellet and tap water ad libitum. To induce osteoarthritis (OA), rats were anesthetized and injected intraarticularly with 50 μL of monosodium iodoacetate (MIA) diluted with saline at a concentration of 60 mg mLG 1 (Sigma, USA) in their right knee joints at day 0 (Al-Saffar et al, 2009).…”

Section: Materials and Methods Experimental Designmentioning

confidence: 99%

Effects on Renal Histology, Renal Metabolizing Enzymes and Oxidative Stress Biomarkers in Rats Administered Zerumbone Against Osteoarthritis

Saffar¹

2015

Asian J. of Cell Biology

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The study aimed to assess histology of rat kidneys and explore the renal oxidative stress post treatment with zerumbone via evaluation of some renal metabolizing enzymes and assay of prostaglandin E 2 in the serum. To conduct such research, fifty adult male Sprague Dawley rats were randomly assigned into five equal groups. Rats in the first and second groups were administered 2 mL kgG 1 body weight of 0.2 and 0.4% w/v zerumbone, respectively. Third group were treated with Celecoxib and served as positive control and rats in the fourth group were received corn oil and served as negative control whereas rats of the fifth group were left without treatment as a basal normal group. Microscopic findings revealed normal picture of the renal tissue and no signs of morphological changes. Biochemical analysis showed that the microsomal content of Cytochrome P450 was significantly induced in the first three groups compared to fourth and fifth groups. Activity of cytosolic glutathione-S-transferase enzyme was induced significantly in zerumbone treated groups. Non significant induction of total glutathione enzyme in the animals treated with zerumbone or celecoxib was detected. Similarly, there was no significant elevation in the level of malondialdehyde in the first three groups compared to fourth and fifth groups. Significant reduction of prostaglandin hormone following four weeks of oral administration of zerumbone and celecoxib compared to the negative control group was observed. Interestingly the levels of hormone in second group showed comparable level to those measured in the normal group.

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Collagenase and Sodium Iodoacetate-Induced Experimental Osteoarthritis Model in Sprague Dawley Rats

Cited by 25 publications

References 26 publications

New herbal composition (OA-F2) protects cartilage degeneration in a rat model of collagenase induced osteoarthritis

New herbal composition (OA-F2) protects cartilage degeneration in a rat model of collagenase induced osteoarthritis

Accentuated transdermal application of glucosamine sulphate attenuates experimental osteoarthritis induced by monosodium iodoacetate

Effects on Renal Histology, Renal Metabolizing Enzymes and Oxidative Stress Biomarkers in Rats Administered Zerumbone Against Osteoarthritis

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