Collagenase in the cornea

Itoi, M; Gnädinger, M.C.; Slansky, Harvey H.; Freeman, Melvin L.; Dohlman, Claes H.

doi:10.1016/s0014-4835(69)80050-3

Cited by 54 publications

(19 citation statements)

References 12 publications

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“…Our results are in agreement with previous studies reporting the absence of collagenase production by normal rabbit stromal cells (23) and whole rabbit corneal stroma stripped of epithelium (24). Epithelial cells released enzymatically from other tissues, such as tadpole tail fin (25), on the other hand, secreted active collagenase in culture, suggesting significant species or tissue differences.…”

Section: Discussionsupporting

confidence: 92%

“…Epithelial cells released enzymatically from other tissues, such as tadpole tail fin (25), on the other hand, secreted active collagenase in culture, suggesting significant species or tissue differences. Perhaps of more interest, there are also reports of active collagenase production by intact normal rabbit corneal tissue containing epithelium and superficial stroma (24), as well as other whole tissue explants with epithelial and connective tissue cells intact, such as skin from human (26), guinea pig and rabbit (3), and tadpole (25). These observations, when compared with our culture data, suggest that at least in the case of the rabbit cornea, coculture of normal stromal and epithelial cells requires an additional stimulus for collagenase production.…”

Section: Discussionmentioning

confidence: 99%

“…During the first 4-hr period at 370C, epithelial cells began attaching to the plastic. Essentially complete attachment of both cell types had occurred by 24 hr. Stromal cells and epithelial cells were cultured separately under identical conditions and attached in the same time periods.…”

Section: Materials and Methods Preparation Of Corneal Epithelial And mentioning

confidence: 99%

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Regulation of corneal collagenase production: epithelial-stromal cell interactions.

Johnson-Muller¹,

Gross²

1978

Proc. Natl. Acad. Sci. U.S.A.

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ABS RACTMixtures of epithelial and stromal cells isolated from normal adult rabbit cornea, when cocultured in the presence of cytochalasin B, produced latent collagenase, whereas neither cell type alone, nor the mixture in the absence of this agent, did so. The enzyme, a characteristic animal collagenase, required proteolytic activation. The relative concentrations of epithelial and stromal cells had a profound effect on collagenase production, the enzyme activity being directly proportional to the number of stromal cells but inversely proportional to the number of epithelial cells. The amount of enzyme released into the medium was also directly proportional to cytochalasin B concentration. Media conditioned by cytochalasin B-treated epithelial or stromal cells did not stimulate collagenase secretion by the other cell type. The data suggest direct cell contact or close proximity as the mode of productive interaction and tentative identify the stromal cell as the source of enzyme and the epithelial cell as a stimulator.Degradation of collagen in the development, remodeling, and repair of mesenchymal tissues requires the controlled action of a group of specific enzymes capable of selectively cleaving this fibrous protein (1, 2).The possibility that interactions between two different cell types might significantly modulate the production or activation of collagenase was suggested by Grillo and Gross (3), who described collagenolytic activity in the edge of healing skin wounds in the guinea pig. They noted that sheets of epithelium isolated from the wound edge, when recombined with granulation tissue from the same region, resulted in significantly more activity than that produced by either tissue alone; epithelium and wound mesenchyme cultured separately were poor or erratic producers of collagenase. More recently, the ability of blood monocytic cell populations to induce or stimulate collagenase production by macrophages (4) and human rheumatoid synovial cells (5, 6) has been described. Soluble secreted factors appear to be the mediating agent here rather than cell-to-cell contact. Various biological and chemical agents such as neutral proteases (7), prostaglandins (8), bacterial endotoxins (9), colchicine (10), phorbol myristic acid (C. E. Brinckerhoff and E. D. Harris, Jr., personal communication), and cytochalasin B (11) are also known to stimulate collagenase production by a number of cultured cell types. The enzyme secreted into the medium in these cell cultures is usually in an inactive form that can be activated by exposure to proteases such as trypsin (12-15) or plasmin (16) and by certain organic mercurial compounds (17).The rabbit cornea proved to be a particularly useful tissue for the study of collagenase regulation by epithelial-mesenchymal cell interaction because the two populations may be readily separated with essentially no cross contamination and both will spread and proliferate rapidly in culture. Neither epithelial nor stromal cells from normal corneas nor mixtures thereof secrete detectable co...

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Regulation of corneal collagenase production: epithelial-stromal cell interactions.

Johnson-Muller¹,

Gross²

1978

Proc. Natl. Acad. Sci. U.S.A.

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“…Cysteine hydrochloride seems to be the most potent inhibitor of collagenase which may be activated in alkali-burned corneas by the regrowing epithelial cells and by the inflammatory cells invading corneal stroma [Br o w n et al, 1969[Br o w n et al, ,1972 Itoi et al, 1969;P fister et al, 1971 ;Slansky et al, 1969Slansky et al, , 1970K rause and N evasaari, 1972; F ra n ço is and C ambie, 1972].…”

Section: Introductionmentioning

confidence: 99%

Intraocular Penetration of Cysteine from Hydrophilic Gel Contact Lenses through the Intact, Epithelium-Denuded and Lime-Burned Cornea

Krejcí¹,

Praus²,

Brettschneider³

1976

Ophthalmic Res

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Hydrophilic gel contact lenses made of poly(hydroxyethylmethacrylate) saturated with a 1% solution of cysteine hydrochloride containing cysteine-³⁵S were applied for 3 h onto rabbit corneas either with intact, scarified, or lime-burned epithelium. Cysteine was found to be easily released from the contact lens and the radioactivity measurements showed increased penetration of cysteine across epithelium-denuded corneas as compared with intact corneas. In lime-burned corneas the cysteine penetration was nearly five times higher in comparison with the values obtained for intact corneas. The rapid intraocular penetration of cysteine from the hydrophilic contact lenses may be useful in clinical practice to prevent the evolution of degenerative changes of eye tissues caused by alkali burns.

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“…The activation of various hydrolytic enzymes has also been implicated in certain ocular disorders, e.g. in the ulcerated cornea and in the retina of the dystrophic rat [Itoi et al, 1969;Burden et ah, 1971], The presence of granular leucocytes in the mechanically injured lens has been reported [Unakar et ah, 1973]. It has been suggested that liberation of granules from the leucocytes followed by extracellular release of hydrolytic enzymes, which are normally confined to the granules, may play a role in the process of wound healing [Ross, 1968;G raf et a!., 1962;Wright and Malawista, 1972], This paper is concerned with the role of granular leucocytes in the injured rabbit lens and particularly with the histochemical localization of an acid hydrolase, i.e.…”

Section: Introductionmentioning

confidence: 99%

Histochemical Localization of Acid Phosphatase in the Injured Rabbit Lens

Unakar¹,

Binder²,

Reddan³

1975

Ophthalmic Res

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The role of granular leucocytes, particularly neutrophils, during wound healing in the injured rabbit lens and the distribution of acid phosphatase were investigated using electron microscopic histochemistry. Granular leucocytes first appeared in the wound coagulum approximately after 12 h following injury. They contained granules which gave a positive reaction for acid phosphatase. At 12 and 24 h acid phosphatase localization was found to be extracellular and was most pronounced at the apical region of the epithelial cells. This localization was most intense at the immediate periphery of the wound and decreased with distance from the site of injury. At 48 h following injury this extracellular localization decreased. The possible role of hydrolytic enzymes in tissue repair is discussed.

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Collagenase in the cornea

Cited by 54 publications

References 12 publications

Regulation of corneal collagenase production: epithelial-stromal cell interactions.

Regulation of corneal collagenase production: epithelial-stromal cell interactions.

Intraocular Penetration of Cysteine from Hydrophilic Gel Contact Lenses through the Intact, Epithelium-Denuded and Lime-Burned Cornea

Histochemical Localization of Acid Phosphatase in the Injured Rabbit Lens

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