Collagenases and Elastases

Mandl, Ines

doi:10.1002/9780470122686.ch5

Cited by 26 publications

(10 citation statements)

References 295 publications

(115 reference statements)

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“…Qualities such as texture or grain size, iron content and plasticity (Hodgskiss, 2006;Sagona, 2003;Wadley et al, 2009;Watts, 2002) may have been more desired than colour, depending on the end product for which the ochre was intended. High iron content, for example, preserves organic materials such as leather (Camerona, 1991;Jones, 2009) and ochre has been used as an aid in tanning and working hides (Audouin and Plisson, 1982;Weedman, 2002a, 2002b;Keeley, 1978 cited in Volman, in press;Mandl, 1961;Velo, 1984). Other recorded ethnographic uses of ochre include: body paint for protection from the sun, cold and insects (Bonwick, 1898, cited in Volman, in press;Ling Roth, 1890, cited in Volman, in press;Marshall, 1976;Pitsi, cited in Wadley, 2001: 7;Watts, 1998); an internal and external medicine (Audouin and Plisson, 1982;Peile, 1979;Velo, 1984); an aggregate in adhesives with which to haft tools (Allain and Rigaud, 1986;Gibson et al, 2004;Hodgskiss, 2006;Lombard, 2002Lombard, , 2004aLombard, , b, 2005Lombard, , 2006Lombard, , 2007Rots, 2002;Wadley, 2005bWadley, , 2006Wadley et al, 2004Wadley et al, , 2009Williamson, 2000); a deodorant on human skin and a waterproofing and protecting agent for wood (Chase, 2008).…”

Section: Introductionmentioning

confidence: 98%

Identifying grinding, scoring and rubbing use-wear on experimental ochre pieces

Hodgskiss

2010

Journal of Archaeological Science

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Section: Introductionmentioning

confidence: 98%

Identifying grinding, scoring and rubbing use-wear on experimental ochre pieces

Hodgskiss

2010

Journal of Archaeological Science

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“…The collagenolytic activity was measured following the procedure described in Reference [65] using a modification of the collagen digestion method, in which the enzymes were incubated for 5 h with native bovine Achilles tendon collagen (Sigma-Aldrich, St. Louis, MO, USA) at 37 °C. The collagen digestion was determined using the colorimetric ninhydrin process [66].…”

Section: Methodsmentioning

confidence: 99%

Vibrio Proteases for Biomedical Applications: Modulating the Proteolytic Secretome of V. alginolyticus and V. parahaemolyticus for Improved Enzymes Production

Salamone¹,

Nicosia

Ghersi

et al. 2019

Microorganisms

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Proteolytic enzymes are of great interest for biotechnological purposes, and their large-scale production, as well as the discovery of strains producing new molecules, is a relevant issue. Collagenases are employed for biomedical and pharmaceutical purposes. The high specificity of collagenase-based preparations toward the substrate strongly relies on the enzyme purity. However, the overall activity may depend on the cooperation with other proteases, the presence of which may be essential for the overall enzymatic activity, but potentially harmful for cells and tissues. Vibrios produce some of the most promising bacterial proteases (including collagenases), and their exo-proteome includes several enzymes with different substrate specificities, the production and relative abundances of which strongly depend on growth conditions. We evaluated the effects of different media compositions on the proteolytic exo-proteome of Vibrio alginolyticus and its closely relative Vibrio parahaemolyticus, in order to improve the overall proteases production, as well as the yield of the desired enzymes subset. Substantial biological responses were achieved with all media, which allowed defining culture conditions for targeted improvement of selected enzyme classes, besides giving insights in possible regulatory mechanisms. In particular, we focused our efforts on collagenases production, because of the growing biotechnological interest due to their pharmaceutical/biomedical applications.

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“…Keeley 1980: 172;Audoin & Plisson 1982;Velo 1984;Couraud 1988;discussion in Watts 2002 andWadley 2005). This argument is sometimes based on second-hand interpretations of Mandl (1961), who argued that metal ions can inhibit the production of collagenase, an enzyme attacking the hide collagen. Experiments conducted by Audoin and Plisson (1982) also seem to validate this hypothesis.…”

Section: Hide-processing and Ochrementioning

confidence: 99%

Ochre and hide-working at a Natufian burial place

Dubreuil

Grosman

2009

Antiquity

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Particular stones found on Epi-Palaeolithic sites in the Levant are thought to be for grinding vegetable matter and to be essential instruments in the development of food processing. Finding an assemblage of these tools in a burial cave, the authors ask a harder question: could they have been used for processing hides with ochre? Use-wear analysis allows a positive verdict, and so the tools take their place in the ritual apparatus associated with burial.

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Collagenases and Elastases

Cited by 26 publications

References 295 publications

Identifying grinding, scoring and rubbing use-wear on experimental ochre pieces

Identifying grinding, scoring and rubbing use-wear on experimental ochre pieces

Vibrio Proteases for Biomedical Applications: Modulating the Proteolytic Secretome of V. alginolyticus and V. parahaemolyticus for Improved Enzymes Production

Ochre and hide-working at a Natufian burial place

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