Collapse-free thermal bonding technique for large area microchambers in plastic lab-on-a-chip applications

Kim, Dong Sung; Lee, Hyun Sup; Han, Jungyoup; Lee, Se Hwan; Ahn, Chong H.; Kwon, Tai Hun

doi:10.1007/s00542-007-0416-z

Cited by 18 publications

(8 citation statements)

References 10 publications

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“…Among several thermoplastics, cyclic olefin copolymers (COC) and polymethylmethacrylate (PMMA) are frequently exploited for making microfluidic devices. These devices were often fabricated by bonding a cover sheet with a substrate containing microchannels and other microfeatures using various bonding methods including thermal fusion,3-7 solvent bonding,8-14 surface treatment,15-20 and adhesives 21, 22. Each of these methods has advantages and disadvantages as reviewed in the literature 1, 2…”

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confidence: 99%

Chemical-Assisted Bonding of Thermoplastics/Elastomer for Fabricating Microfluidic Valves

Liu

Chen

et al. 2010

Anal. Chem.

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Thermoplastics such as cyclic olefin copolymer (COC) and polymethylmethacrylate (PMMA) have been increasingly used in fabricating microfluidic devices. However, the state-of-the-art microvalve technology is a polydimethylsiloxane (PDMS)-based three-layer structure. In order to integrate such a valve with a thermoplastics-based microfluidic device, a bonding method for thermoplastics/PDMS must be developed. We report here a method to bond COC with PDMS through surface activation by corona discharge, surface modification using 3-(trimethoxysilyl)propyl methacrylate (TMSPMA), and thermal annealing. The method is also applicable to PMMA. The bonding strength between thermoplastics and PDMS was represented by the peeling force, which was measured using a method established by the International Organization for Standardization (ISO). The bonding strength measurement offered an objective and quantitative indicator for protocol optimization, as well as comparison with other PDMSassociated bonding methods. Using optimized bonding conditions, two valve arrays were fabricated in a COC/PDMS/COC device and cyclic operations of valve closing/opening were successfully demonstrated. The valve-containing devices withstood 100 psi (~689 KPa) without delamination. Further, we integrated such valve arrays in a device for protein separation and demonstrated isoelectric focusing in the presence of valves.Thermoplastics have been increasingly employed for microfluidics applications. 1 Compared with glass and silicon, thermoplastics offer several advantages including manufacturability, low cost, and biocompatibility. [1][2][3][4] Among several thermoplastics, cyclic olefin copolymers (COC) and polymethylmethacrylate (PMMA) are frequently exploited for making microfluidic devices. These devices were often fabricated by bonding a cover sheet with a substrate containing microchannels and other microfeatures using various bonding methods including thermal fusion, 3-7 solvent bonding, [8][9][10][11][12][13][14] surface treatment, [15][16][17][18][19][20] and adhesives. 21,22 Each of these methods has advantages and disadvantages as reviewed in the literature. 1,2 A microfluidic system consists of a number of necessary building blocks. One of them is microvalves that may regulate flows, contain fluids, and isolate one region from the other. 23,24 Microvalves can be actuated using a variety of mechanisms, including electric, pneumatic, and thermal methods. 23 The state-of-the-art microvalve technique for lab-on-achip systems is elastic membrane-based polydimethylsiloxane (PDMS) valves. Quake's * To whom correspondence should be addressed. hfan@ufl.edu; Fax: 352-392-7303. . 25 followed by several studies using thousands of integrated microvalves for high-throughput applications. [26][27][28][29] The elastomer valves consists of three layers of PDMS. Control channels in the top layer are used to regulate the fluid channels in the bottom layer through the elastic property of the middle PDMS layer. The elastomer valve has been adopted by...

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Chemical-Assisted Bonding of Thermoplastics/Elastomer for Fabricating Microfluidic Valves

Liu

Chen

et al. 2010

Anal. Chem.

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“…Subsequently, the structured substrate was covered with a flat PS substrate via collapse-free thermal bonding technique. 20 To verify moldability of the LOD designs, injection molding using metal mold has been conducted to fabricate the preliminary LOD containing the C-MUFID and the CSM on a trial basis. Consequently, injection molding of the preliminary LOD was successfully achieved as described in supplementary Fig.…”

Section: A Lod Fabricationmentioning

confidence: 99%

Centrifugal multiplexing fixed-volume dispenser on a plastic lab-on-a-disk for parallel biochemical single-end-point assays

Park

Kim

2015

Biomicrofluidics

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In this study, a multiple sample dispenser for precisely metered fixed volumes was successfully designed, fabricated, and fully characterized on a plastic centrifugal lab-on-a-disk (LOD) for parallel biochemical single-end-point assays. The dispenser, namely, a centrifugal multiplexing fixed-volume dispenser (C-MUFID) was designed with microfluidic structures based on the theoretical modeling about a centrifugal circumferential filling flow. The designed LODs were fabricated with a polystyrene substrate through micromachining and they were thermally bonded with a flat substrate. Furthermore, six parallel metering and dispensing assays were conducted at the same fixed-volume (1.27 μl) with a relative variation of ±0.02 μl. Moreover, the samples were metered and dispensed at different sub-volumes. To visualize the metering and dispensing performances, the C-MUFID was integrated with a serpentine micromixer during parallel centrifugal mixing tests. Parallel biochemical single-end-point assays were successfully conducted on the developed LOD using a standard serum with albumin, glucose, and total protein reagents. The developed LOD could be widely applied to various biochemical single-end-point assays which require different volume ratios of the sample and reagent by controlling the design of the C-MUFID. The proposed LOD is feasible for point-of-care diagnostics because of its mass-producible structures, reliable metering/dispensing performance, and parallel biochemical single-end-point assays, which can identify numerous biochemical.

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“…Among various polymeric materials suitable for micromolding of microfluidic chips, the cyclic olefin copolymer (COC) shows several excellent properties, for instance good moldability, high transparency, biocompatibility, and so forth. Furthermore, the molded COC articles can be easily bonded together by a thermal bonding technique [20], which is essential for constructing closed microfluidic devices. In this regard, COC (Topas 5013, Ticona) was selected as a molding material in the present study.…”

Section: Microinjection Molding Processmentioning

confidence: 99%

A novel monolithic fabrication method for a plastic microfluidic chip with liquid interconnecting ports

Lee¹,

Kwon²

2010

J. Micromech. Microeng.

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In the present study, a novel monolithic fabrication method was developed for the manufacturing of a plastic microfluidic chip with liquid interconnecting ports. As the present method can realize both interconnecting ports and through holes, which are essential components for the delivery of working fluids, in the plastic microfluidic chip, no additional processes and external ports are required. Furthermore, the connection of external silicone tubing can be simply achieved by utilizing an elastic deformation of the used tubing. As one representative example, a microinjection molding of a prototype microfluidic chip having two types of interconnecting ports was demonstrated. After obtaining upper and lower plates by the microinjection molding process utilizing mold cores with pin structures, the thermal bonding of the molded plates was carried out, resulting in the prototype plastic microfluidic chip with interconnecting ports. From microfluidic experiments using the fabricated prototype, it was found that the present method could be quite useful in various microfluidic applications.

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Collapse-free thermal bonding technique for large area microchambers in plastic lab-on-a-chip applications

Cited by 18 publications

References 10 publications

Chemical-Assisted Bonding of Thermoplastics/Elastomer for Fabricating Microfluidic Valves

Chemical-Assisted Bonding of Thermoplastics/Elastomer for Fabricating Microfluidic Valves

Centrifugal multiplexing fixed-volume dispenser on a plastic lab-on-a-disk for parallel biochemical single-end-point assays

A novel monolithic fabrication method for a plastic microfluidic chip with liquid interconnecting ports

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