Collared Pecary (tayassu tajacu) as a new model of renal ischemic injury induced by clamping the renal artery

Abstract

PURPOSE:The use of the collared peccary as an experimental model for ischemic nephropathy. METHODS:A total of 12 collared peccary (Tayassu tajacu) was used and ischemic nephropathy was induced in six of these animals that constituted the experimental group (G1) while the other six formed the control group (G2). Ischemic nephropathy was induced surgically by partial occlusion of the left renal artery. The disease course was assessed by hematological tests, serum chemistry, urinalysis, ultrasound (US) and doppler ultrasound function of the renal artery before induction, and at five, 10, 15 and 20 days after surgery.Twenty days after the occlusion, unilateral nephrectomy and histopathological examination were performed to assess renal morphology. RESULTS:Statistical analysis by Fischer's test showed a significant difference (p<0.05) between the control group and the experimental group. The histopathological examination showed glomerular, tubular and interstitial lesions. In the experimental group, 83.3% (5 /6) showed moderate renal lesions and only 16.7% (1/6) were classified with no lesions. The ultrasound examination of the right kidney presented statistical difference between day 5 and day 10 post occlusion. CONCLUSION:The collared peccary as a good experimental model for ischemic renal disease, because it could be manipulated during the research time without death, with health conditions that permit any subsequent procedure for disease therapy.Key words: Artiodactyla. Kidney Diseases. Ischemia. Models, Animal. Collared Pecary (tayassu tajacu) as a new model of renal ischemic injury induced by clamping the renal arteryActa Cirúrgica 2014 -561

B-mode and Doppler ultrasonography of abdominal structures of the peccary (Tayassu tajacu, Linnaeus 1758)

“…The anesthetic protocol used in this study produced adequate sedation without hemodynamic compromise, which would alter the flow measurements taken (all animals were monitored for cardiovascular changes using an electrocardiogram and blood pressure measurements). This anesthetic protocol was used with great success in the peccary by Pessoa et al, (2014) to collect adipose tissue, by Argôlo Neto et al, (2016) to collect bone marrow, and by Bezerra et al, (2014) in establishing a preclinical model of renal injury from renal artery clamping. Batista et al, (2009) reported that chemical tranquilization in the peccary significantly reduces heart rate, rectal temperature, biochemical parameters, and stress indicators, making it easier to manipulate the animal.…”

Echocardiographic reference ranges for sedated healthy peccaries ( Tayassu tajacu, Linnaeus , 1758 )

“…Peccaries (Tayassu tajacu, Linnaeus, 1758) are wild mammals belonging to the Tayassuidae family and Tayassu genus. They are found experimental models for evaluating the evolution of diseases, peccaries are a good alternative model for scientific investigations (Bezerra et al, 2014).…”

Assessment of the vertebral heart scale and cardiothoracic ratio to standardize the heart size of collared peccaries (Tayassu tajacu Linnaeus, 1758) restrained with ketamine and midazolam

“…The collared peccary is highly adaptable to different environments, extremely robust and resistant to various diseases. These characteristics greatly favor their use in scientific investigations, as observed by Bezerra et al (2014) and Pessoa et al (2014), which used these animals as models for renal ischemic lesions and the isolation of mesenchymal cells derived from adipose tissue. Thus, these animals are more accessible alternatives for scientific studies than is the domestic pig (Sus scrofa domesticus) (Monteiro et al 2009) or mini pigs (Vodička et al 2005).…”