Collateral Sprouting of Peripheral Sensory Neurons Exhibits a Unique Transcriptomic Profile

Lemaitre, Dominique; Hurtado, Maica Llavero; Gregorio, Cristian De; Oñate, Maritza; Martínez, Gabriela; Catenaccio, Alejandra; Wishart, Thomas M.; Court, Felipe A.

doi:10.1007/s12035-020-01986-3

Cited by 19 publications

(15 citation statements)

References 57 publications

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“…Interestingly, our manipulations of the PTEN/mTOR pathway and resulting effects on protein synthesis by overexpression and inhibition of miR21 and miR-199a-3p selectively affected axon elongation rather than axon branching. Transcriptional programs associated with sprouting or branching of sensory neurons have been reported for both in vivo and cultured neurons ( Harrison et al, 2015 ; Lemaitre et al, 2020 ). In contrast to these findings, our data emphasize that the elongating axon growth regulated by the balance of miR-21 and miR-199a-3p levels is impacted by direct posttranscriptional regulation of Pten and mTor mRNAs.…”

Section: Discussionmentioning

confidence: 99%

MicroRNAs 21 and 199a-3p Regulate Axon Growth Potential through Modulation ofPtenandmTor mRNAs

Kar

Lee

Sahoo

et al. 2021

eNeuro

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Section: Discussionmentioning

confidence: 99%

MicroRNAs 21 and 199a-3p Regulate Axon Growth Potential through Modulation ofPtenandmTor mRNAs

Kar

Lee

Sahoo

et al. 2021

eNeuro

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“…Finally, compensatory circuits generated by collateral sprouting might play a role in the rather good subjective outcome of the patients. Given that collateral sprouting is triggered by signals from damaged fibers during the Wallerian degeneration process, it should definitely be considered when the nerve transfer has been done early after the initial injury [ 51 ]. However, when the recipient nerve has been reinnervated after chronic impairment, this phenomenon is less likely to occur at the targeted site.…”

Section: Discussionmentioning

confidence: 99%

Sensory Neurotization of the Ulnar Nerve, Surgical Techniques and Functional Outcomes: A Review

Nguyễn

Pierrart²,

Crenn

2022

JCM

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When ulnar nerve lesions happen above the wrist level, sensation recovery after acute repair or nerve grafting is often challenging. Distal sensory nerve transfers may be an option for overcoming these sequelae. However, little data has been published on this topic. This study aims to review the surgical procedures currently proposed, along with their functional results. Six donor nerves have been described at the wrist level: the palmar branch of the median nerve, the cutaneous branch of the median nerve to the palm with or without fascicles of the ulnar digital nerve of the index finger, the posterior interosseous nerve, the third palmar digital nerve, the radial branch of the superficial radial nerve, the median nerve, and the fascicule for the third web space. Three donor nerves have been reported at the hand level: the ulnar digital nerves of the index, and the radial or ulnar digital nerves of the long finger. Three target sites were used: the superficial branch of the ulnar nerve, the dorsal branch of the ulnar nerve, and the ulnar digital branch of the fifth digit. All the technical points have been illustrated with anatomical dissection pictures. After assessing sensory recovery using the British Medical Research Council scale, a majority of excellent recoveries scaled S3+ or S4 have been reported in the targeted territory for each technique.

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“…Indeed, in the absence of Ctgf, axonal re-growth is slower and appears less polarized, likely due to a slower and misdirected migration of SCs within the bridge, eventually leading to a delay in neurotransmission rescue. Of note, the Ctgf transcript increases in neurons responsible for collateral sprouting, which is a process where undamaged neurons react to an injury-induced environment (Wallerian degeneration) by expanding sprouts that functionally synapse with denervated targets [ 59 ]. Along the same line, Ctgf-positive sprouts, which form in PSCs upon neurotransmission blockade (by either denervation, or intoxication), further support the view that SC-expressed Ctgf may play a role in supporting/guiding axonal elongation.…”

Section: Discussionmentioning

confidence: 99%

Hydrogen peroxide induced by nerve injury promotes axon regeneration via connective tissue growth factor

Negro

Lauria²,

Stazi

et al. 2022

acta neuropathol commun

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Regeneration of the neuromuscular junction (NMJ) leverages on extensive exchange of factors released from motor axon terminals (MATs), muscle fibers and perisynaptic Schwann cells (PSCs), among which hydrogen peroxide (H2O2) is a major pro-regenerative signal. To identify critical determinants of NMJ remodeling in response to injury, we performed temporal transcriptional profiling of NMJs from 2 month-old mice during MAT degeneration/regeneration, and cross-referenced the differentially expressed genes with those elicited by H2O2 in SCs. We identified an enrichment in extracellular matrix (ECM) transcripts, including Connective Tissue Growth Factor (Ctgf), which is usually expressed during development. We discovered that Ctgf levels are increased in a Yes-associated protein (YAP)-dependent fashion in response to rapid, local H2O2 signaling generated by stressed mitochondria in the injured sciatic nerve, a finding highlighting the importance of signals triggered by mechanical force to motor nerve repair. Through sequestration of Ctgf or inactivation of H2O2, we delayed the recovery of neuromuscular function by impairing SC migration and, in turn, axon-oriented re-growth. These data indicate that H2O2 and its downstream effector Ctgf are pro-regenerative factors that enable axonal growth, and reveal a striking ECM remodeling process during nerve regeneration upon local H2O2 signaling. Our study identifies key transcriptomic changes at the regenerating NMJ, providing a rich source of pro-regenerative factors with potential for alleviating the consequences of peripheral nerve injuries.

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Collateral Sprouting of Peripheral Sensory Neurons Exhibits a Unique Transcriptomic Profile

Cited by 19 publications

References 57 publications

MicroRNAs 21 and 199a-3p Regulate Axon Growth Potential through Modulation ofPtenandmTor mRNAs

MicroRNAs 21 and 199a-3p Regulate Axon Growth Potential through Modulation ofPtenandmTor mRNAs

Sensory Neurotization of the Ulnar Nerve, Surgical Techniques and Functional Outcomes: A Review

Hydrogen peroxide induced by nerve injury promotes axon regeneration via connective tissue growth factor

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