Workers’ attitudes towards people with disabilities can influence labor inclusion. Despite the relevance of learning about these attitudes, in Chile, there are no reliable and validated instruments to measure them in workers. An instrumental study was carried out to adapt and psychometrically analyze the Spanish version of the Scale of Attitudes towards People with Disabilities (EAPCD) in the Chilean population. By means of a non-probabilistic convenience sampling, 310 people between 19 and 76 years of age (M = 39.4; SD = 11.2) from different regions of Chile participated in the study. The linguistic adaptation included the change in Spanish idioms and expressions to the Chilean context, whose relevance was validated with cognitive interviews. Descriptive analysis, internal consistency analysis, and CFA (confirmatory factor analysis) were performed. The CFA indicated an adequate fit to the three-factor model proposed in the original scale (χ2(431) = 808.070, p < 0.01; RMSEA = 0.053; CFI = 0.93; TLI = 0.93). The Social Relations and Normalization dimensions were reliable; however, it is recommended that the items of the Intervention Programs dimension be revised or eliminated, since they could be confusing in the local context. It is concluded that the scale is reliable and valid to evaluate attitudes towards the inclusion of people with disabilities in Chile in the work context.