Collecting and deactivating TGF-β1 hydrogel for anti-scarring therapy in post-glaucoma filtration surgery

Wang, Ruiqi; Chen, Boyang; Wei, Haiying; Yan, Wei; Wu, Yuping; Cao, Wang Fang; Zhang, Bosong; Liu, Fengzhen; Tian, Hui; Chen, Daniel; Tian, Weiming

doi:10.1016/j.mtbio.2022.100260

Cited by 6 publications

(5 citation statements)

References 74 publications

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“…The contact of the plate’s surface with the sclera tissue moderates fibroblast proliferation due to mild corrosion of HA-Mg degradation [ 11 ], effectively preventing subconjunctival scarring once the drainage plate is fully degraded. Notably, the postoperative scar formation typically takes more than 2 weeks, usually extending around 15 weeks [ 30 ]. This process seems to coincide with the degradation cycle of HA-Mg drainage plate.…”

Section: Discussionmentioning

confidence: 99%

Intraocular pressure control efficacy and safety of HA-Mg glaucoma drainage plate implantation in the anterior chamber of rabbit eyes

Cai,

Luo,

Feng

et al. 2024

J Mater Sci: Mater Med

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The current clinical application of glaucoma drainage devices is made of non-degradable materials. These non-degradable drainage devices often trigger inflammatory responses and scar proliferation, possibly leading to surgical failure. We developed a biodegradable material hydroxyapatite-coated magnesium (HA-Mg) as a glaucoma drainage device. Twelve New Zealand white rabbits were randomly assigned to three groups: HA-Mg drainage plate group (6 right eyes), trabeculectomy group (6 right eyes), and control group (12 left eyes). Results showed that all HA-Mg drainage plates were completely degraded ~4 months postoperatively. At the 5th month postoperatively, there was no statistical difference in the corneal endothelium density between the HA-Mg drainage plate group and the control group (p = 0.857). The intraocular pressure (IOP) level in the HA-Mg drainage plate implantation group was lower than in the other two groups. The trypan blue dye still drained from the anterior chamber to the subconjunctiva 5 months after HA-Mg drainage plate implantation. HE staining revealed the scleral linear aqueous humor drainage channel and anterior synechia were observed after drainage plate completely degraded, with no obvious infiltration with the inflammatory cells. This study showed the safety and efficacy of HA-Mg glaucoma drainage plate in controlling IOP after implantation into the anterior chamber of rabbit eyes. Graphical abstract

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Section: Discussionmentioning

confidence: 99%

Intraocular pressure control efficacy and safety of HA-Mg glaucoma drainage plate implantation in the anterior chamber of rabbit eyes

Cai,

Luo,

Feng

et al. 2024

J Mater Sci: Mater Med

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“…The RAW264.7 cells were seeded in 6 well plates with a cell density of 1 × 10 4 cells per well and incubated for 24 h in an incubator. After 24 h, cells were treated with 1 µg/mL concentration of lipopolysaccharides (LPS) in DMEM with 10% FBS then cells were incubated for 24 h. After that, cells were treated with different concentrations of formulation and incubated for 24 h. After 24 h, cells were taken, and RNA was isolated by using the TRIzol method, and cytokinin expression was determined by using RT-PCR [40,41]. Briefly, Total cellular RNA was isolated from controltreated groups of cells using the TRIzol reagent.…”

Section: Biocompatibility Studymentioning

confidence: 99%

ECM-mimetic, NSAIDs loaded thermo-responsive, immunomodulatory hydrogel for rheumatoid arthritis treatment

Shah,

Ghosh,

et al. 2024

BMC Biotechnol

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Background Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease, and it leads to irreversible inflammation in intra-articular joints. Current treatment approaches for RA include non-steroidal anti-inflammatory drugs (NSAIDs), disease-modifying anti-rheumatic drugs (DMARDs), corticosteroids, and biological agents. To overcome the drug-associated toxicity of conventional therapy and transdermal tissue barrier, an injectable NSAID-loaded hydrogel system was developed and explored its efficacy. Results The surface morphology and porosity of the hydrogels indicate that they mimic the natural ECM, which is greatly beneficial for tissue healing. Further, NSAIDs, i.e., diclofenac sodium, were loaded into the hydrogel, and the in vitro drug release pattern was found to be burst release for 24 h and subsequently sustainable release of 50% drug up to 10 days. The DPPH assay revealed that the hydrogels have good radical scavenging activity. The biocompatibility study carried out by MTT assay proved good biocompatibility and anti-inflammatory activity of the hydrogels was carried out by gene expression study in RAW 264.7 cells, which indicate the downregulation of several key inflammatory genes such as COX-2, TNF-α & 18s. Conclusion In summary, the proposed ECM-mimetic, thermo-sensitive in situ hydrogels may be utilized for intra-articular inflammation modulation and can be beneficial by reducing the frequency of medication and providing optimum lubrication at intra-articular joints. Graphical Abstract

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“…Multiple publications report lowered sTWEAK as a biomarker of atherosclerosis-associated diseases, which could be a possible link with the atherosclerotic theory of glaucoma. [106][107][108][109][110] TRAIL is a cytokine that plays an important role in regulating innate and adaptive immune systems. Similarly to TRAIL and other TNFSFs, it can be expressed as a trans-membrane protein (ie on epithelial, mucosal cells, neurons, and oligodendrocytes) and as a soluble form (sTRAIL).…”

Section: Tweak and Trailmentioning

confidence: 99%

“…TGF-β1 plays a crucial role, eg in scar formation after glaucoma surgery. 106 TGF-β1 is also associated with the pathogenesis of pseudoexfoliation glaucoma since it activates TGF-βpathway. 135 In neuroinflammation and neurodegeneration, TGF-β1 is linked with possible Alzheimer's disease pathogenesis.…”

Section: Lap Tgf-beta1mentioning

confidence: 99%

Inflammation and Neurodegeneration in Glaucoma: Isolated Eye Disease or a Part of a Systemic Disorder? - Serum Proteomic Analysis

Okruszko,

Szabłowski,

Zarzecki

et al. 2024

JIR

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Glaucoma is the most common optic neuropathy and the leading cause of irreversible blindness worldwide, which affects 3.54% of the population aged 40-80 years. Despite numerous published studies, some aspects of glaucoma pathogenesis, serum biomarkers, and their potential link with other diseases remain unclear. Recent articles have proposed that autoimmune, oxidative stress and inflammation may be involved in the pathogenesis of glaucoma. Methods: We investigated the serum expression of 92 inflammatory and neurotrophic factors in glaucoma patients. The study group consisted of 26 glaucoma patients and 192 healthy subjects based on digital fundography. Results: Patients with glaucoma had significantly lower serum expression of IL-2Rβ, TWEAK, CX3CL1, CD6, CD5, LAP TGF-beta1, LIF-R, TRAIL, NT-3, and CCL23 and significantly higher expression of IL-22Rα1. Conclusion:Our results indicate that patients with glaucoma tend to have lower levels of neuroprotective proteins and higher levels of neuroinflammatory proteins, similar to those observed in psychiatric, neurodegenerative and autoimmune diseases, indicating a potential link between these conditions and glaucoma pathogenesis.

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Collecting and deactivating TGF-β1 hydrogel for anti-scarring therapy in post-glaucoma filtration surgery

Cited by 6 publications

References 74 publications

Intraocular pressure control efficacy and safety of HA-Mg glaucoma drainage plate implantation in the anterior chamber of rabbit eyes

Intraocular pressure control efficacy and safety of HA-Mg glaucoma drainage plate implantation in the anterior chamber of rabbit eyes

ECM-mimetic, NSAIDs loaded thermo-responsive, immunomodulatory hydrogel for rheumatoid arthritis treatment

Inflammation and Neurodegeneration in Glaucoma: Isolated Eye Disease or a Part of a Systemic Disorder? - Serum Proteomic Analysis

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