Collecting fungal mycelium using in-growth mesh bags: Effects of the sand particle size and seasonality

Zuev, Andrey G.; Khmeleva, M.V.; Tiunov, Alexei V.

doi:10.1016/j.pedobi.2019.150591

Cited by 9 publications

(2 citation statements)

References 34 publications

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“…55 Current research on mycelium-based materials lacks essential details on manufacturing processes, material compositions, incubation, and growth conditions because fungal mycelium is a living organism and requires intensive research. 56,57 In contrast to well researched and standardized processes for engineering composites, mycelium-bound composites represent a nowum. According to research, publications report on relatively coarse material compositions for mycelium-bound composites such as rice husks, oat hulls, rapeseed cake, cotton residues, sawdust, leaves and bio-waste, etc.…”

Section: Overview Of Nanomaterials Used In Mycelium-reinforced Nanocompositesmentioning

confidence: 99%

Polyacrylonitrile (PAN) nanofiber mats for mushroom mycelium growth investigations and formation of mycelium-reinforced nanocomposites

Trabelsi

Mamun

Klöcker

et al. 2021

Journal of Engineered Fibers and Fabrics

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Mycelium-bound composites are new environmentally friendly, cost-effective and sustainable materials, enable energy-saving bio-composite fabrication, and provide an alternative to synthetic materials. Current research on mycelium-based composites reports on relatively coarse material compositions such as rice husks, cotton residues, sawdust, leaves and bio-waste, etc. According to research, very few publications report on mycelium-reinforced composites with the use of nanomaterials and this topic is under-researched and this study helps to fill this gap. The focus of this study deals with the preparation of mycelium-reinforced nanocomposites including nanofiber mats and the investigation of the different nanofiber mat morphologies on the growth of fungal mycelium. The mycelium macrofibers from Pleurotus ostreatus fungi were grown on polyacrylonitrile (PAN) nanofiber mats. Different morphologies of nanofiber mats such as fibrous and non-fibrous membrane areas or a mixture of both were used for mycelial growth with an additional nutrient. Moreover, mycelium/PAN nanocomposites were oxidative stabilized and carbonized and mycelium retains its morphology. For faster color differentiation between mycelium and nanofibers, PAN nanofiber mats were dyed in a one-step process by adding dye powder to the electrospinning solution as an additional tool. No significant differences in mycelial growth and morphology were observed regarding the different nanofiber mat types and the use of dye. These mycelium-reinforced nanocomposites are promising for many applications such as medicine and biotechnology, air and water purification and filtration, vertical farming, architecture, etc., and enable energy-saving bio-composite fabrication.

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Section: Overview Of Nanomaterials Used In Mycelium-reinforced Nanocompositesmentioning

confidence: 99%

Polyacrylonitrile (PAN) nanofiber mats for mushroom mycelium growth investigations and formation of mycelium-reinforced nanocomposites

Trabelsi

Mamun

Klöcker

et al. 2021

Journal of Engineered Fibers and Fabrics

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“…The ingrowth bags then are retrieved after an incubation period. This approach has been used to estimate the production, biomass turnover, or composition of fine roots, mycorrhizal fungal dynamics, and bacterial dispersal [ 3 , 11 , 18 , 22 , 61 , 64 , 69 ]. Ingrowth bags have not been well-assessed as a monitoring tool for soil microbial communities and the limitations of using ingrowth bags versus bulk soil have not been evaluated using MinION sequencing.…”

Section: Introductionmentioning

confidence: 99%

Sterile sentinels and MinION sequencing capture active soil microbial communities that differentiate crop rotations

Erlandson,

Ewing,

Osborne

et al. 2024

Environmental Microbiome

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Background Soil microbial communities are difficult to measure and critical to soil processes. The bulk soil microbiome is highly diverse and spatially heterogeneous, which can make it difficult to detect and monitor the responses of microbial communities to differences or changes in management, such as different crop rotations in agricultural research. Sampling a subset of actively growing microbes should promote monitoring how soil microbial communities respond to management by reducing the variation contributed by high microbial spatial and temporal heterogeneity and less active microbes. We tested an in-growth bag method using sterilized soil in root-excluding mesh, “sterile sentinels,” for the capacity to differentiate between crop rotations. We assessed the utility of different incubation times and compared colonized sentinels to concurrently sampled bulk soils for the statistical power to differentiate microbial community composition in low and high diversity crop rotations. We paired this method with Oxford Nanopore MinION sequencing to assess sterile sentinels as a standardized, fast turn-around monitoring method. Results Compared to bulk soil, sentinels provided greater statistical power to distinguish between crop rotations for bacterial communities and equivalent power for fungal communities. The incubation time did not affect the statistical power to detect treatment differences in community composition, although longer incubation time increased total biomass. Bulk and sentinel soil samples contained shared and unique microbial taxa that were differentially abundant between crop rotations. Conclusions Overall, compared to bulk soils, the sentinels captured taxa with copiotrophic or ruderal traits, and plant-associated taxa. The sentinels show promise as a sensitive, scalable method to monitor soil microbial communities and provide information complementary to traditional soil sampling.

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Mycorrhiza-feeding soil invertebrates in two coniferous forests traced with 13C labelling

et al. 2023

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Collecting fungal mycelium using in-growth mesh bags: Effects of the sand particle size and seasonality

Cited by 9 publications

References 34 publications

Polyacrylonitrile (PAN) nanofiber mats for mushroom mycelium growth investigations and formation of mycelium-reinforced nanocomposites

Polyacrylonitrile (PAN) nanofiber mats for mushroom mycelium growth investigations and formation of mycelium-reinforced nanocomposites

Sterile sentinels and MinION sequencing capture active soil microbial communities that differentiate crop rotations

Mycorrhiza-feeding soil invertebrates in two coniferous forests traced with 13C labelling

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