Collection and characterization of banana gene pools (Musa spp.) in Manipur (N.E. India) using PCR–RFLP and RAPD and ISSR markers

Singh, Warepam Amuchou; Singh, Nandeibam Samarjit; Devi, Elangbam Julia; Handique, Pratap Jyoti; Devi, Huidrom Sunitibala

doi:10.1007/s40415-021-00722-y

Cited by 4 publications

(2 citation statements)

References 51 publications

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“…Each molecular marker has its advantages and disadvantages. However, ISSR markers combine the advantages of several molecular markers such as high polymorphism, reproducibility, low cost, ease of use, and no need for genome sequencing (Singh et al 2021). Therefore, the analysis of the genetic diversity of 34 germplasms of E. scandens from different locations using ISSR markers provided the foundation for the identi cation of E. scandens germplasm resources for breeding.…”

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confidence: 99%

Evaluating the genetic diversity of Erythropalum scandens based on using inter-simple sequence repeat markers

Yang

Zhang

Huang

et al. 2023

Genet Resour Crop Evol

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Erythropalum scandens Blume, an emerging medicinal plant with great potential for drug development, also possesses high edible value. In this study, we investigated the genetic diversity of the germplasm of E. scandens obtained from different geographical locations using inter simple sequence repeat (ISSR) markers. For this purpose, 18 ISSR primer pairs with a distinct background and adequate polymorphism were selected. We established an optimal ISSR-PCR reaction system (20 µL) with the following parameters: 1 µL DNA template (60 ng•µL -1 ), 1.2 µL primers (10 µmol•µL -1 ), 10 µL MasterMix, and 7.8 µL H 2 O. A total of 183 loci were ampli ed using the 18 primer pairs, of which 121 (66.12%) indicated polymorphism. Moreover, 34 germplasms of E. scandens exhibited genetic similarity coe cients ranging from 0.7104 to 0.9563, genetic distances ranging from 0.0447 to 0.3420, Nei's genetic diversity index of 0.1946, and Shannon's information index of 0.2982, suggesting high intraspeci c genetic diversity. UPGMA cluster and PCoA analyses distinguished the germplasm of E. scandens obtained from Guangxi from those collected from Guangdong, Hainan, Fujian, and Guizhou. However, the Mantel correlation analysis revealed that the genetic variation among the 34 germplasms of E. scandens was not signi cantly related to geographical distance. The analysis of the genetic background of wild and cultivated germplasms of E. scandens can help guide variety selection and breeding. Furthermore, the present study revealed the genetic background and a nities among 34 germplasms of E. scandens. Overall, our ndings lay the foundation for the conservation and utilization of germplasm resources, identi cation and classi cation of varieties, and variety selection and improvement of E. scandens at the molecular level. are shown in Fig. 2.

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Section: )mentioning

confidence: 99%

Evaluating the genetic diversity of Erythropalum scandens based on using inter-simple sequence repeat markers

Yang

Zhang

Huang

et al. 2023

Genet Resour Crop Evol

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“…Breeding approaches to enhance additional varieties having high yield, high nutritional amount, and resistance to biotic and abiotic stress are essential to compensate for food deficiencies (Mekonnen et al 2022;Biswas et al 2023). Musa cultivars breeding can be problematic and difficult (Singh et al 2021;Hinge et al 2022). The situation was considered impractical essentially because of considerations such as vegetative propagation, the existence of polyploidy level, low fertility of clones, and time required to produce an improved cultivar, preventing fast continue in breeding, particularly now that the problem of climate change is globally threatening harvest produces.…”

Section: Genetic Diversity and Cluster Analysismentioning

confidence: 99%

Molecular evaluation of genetic diversity and relationships of Musa cultivars in Thailand using Start Codon Targeted (SCoT) markers

NITIWORAKARN,

PHAE-NGAM,

VANIJAJIVA

2023

Biodiversitas

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Abstract. Nitiworakarn S, Phae-Ngam W, Vanijajiva O. 2023. Molecular evaluation of genetic diversity and relationships of Musa cultivars in Thailand using Start Codon Targeted (SCoT) markers. Biodiversitas 24: 4060-4068. Musa cultivars are mostly obtained from crossing between wild diploid subspecies of M. acuminata (A genome) and M. balbisiana (B genome), and they reveal numerous levels of ploidy and genomic constitution. The present study, for the first time, investigated the genetic diversity of 90 Musa cultivars from 17 localities of Chai Badan district in Lopburi Province, Thailand, using start codon targeted (SCoT) markers. The SCoT technique has shown very high repeatability for characterizing Musa cultivars. The result indicated that DNA fingerprints from 30 SCoT primers generated 244 amplicons, among which 238 bands (97.54%) were polymorphic, with a mean of 7.93 polymorphic bands per primer. The average polymorphism information content (PIC) was high, ranging from 0.345 to 0.483, with an average of 0.432. Genetic diversity and its partitioning parameters were calculated and demonstrated that present Musa cultivars maintain relatively high genetic diversity. The unweighted pair-group method with an arithmetic cluster analysis, principal coordinate analysis and STRUCTURE analysis results were the same and showed the clear division of the genotypes into two distinct clusters, which were cluster-I consisted of only the AA genomes and cluster-II comprised of ABB and BB genomes. In conclusion, SCoT markers obtained here showed their manipulation in genetic diversity and the relationship of cultivated Musa, which would be practical for genetic preservation and continuing breeding programs in Thailand and other regions.

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Recent Advances in the Use of Molecular Markers for Fruit Crop Improvement

Lalrinmawii,

Mir,

Perveen

2023

Molecular Marker Techniques

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Collection and characterization of banana gene pools (Musa spp.) in Manipur (N.E. India) using PCR–RFLP and RAPD and ISSR markers

Cited by 4 publications

References 51 publications

Evaluating the genetic diversity of Erythropalum scandens based on using inter-simple sequence repeat markers

Evaluating the genetic diversity of Erythropalum scandens based on using inter-simple sequence repeat markers

Molecular evaluation of genetic diversity and relationships of Musa cultivars in Thailand using Start Codon Targeted (SCoT) markers

Recent Advances in the Use of Molecular Markers for Fruit Crop Improvement

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