Collection and use of dry pollen for pollination of kiwifruit

King, M. J.; Ferguson, A. M.

doi:10.1080/01140671.1991.10422881

Cited by 10 publications

(11 citation statements)

References 2 publications

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“…As an alternative to hand pollination mechanical methods are being considered. Traditionally, pollen was suspended in a wet fluid carrier (Hopping & Jerram, 1980;Hopping & Simpson, 1982;Hopping & Hachng, 1983), but new systems using dry pollen have being assayed (King & Ferguson, 1991). The present work shows results obtained with a mechanical system that uses fresh pollen.…”

Section: Discussionmentioning

confidence: 77%

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Influence of pollination systems on fruit set and fruit quality in kiwifruit (Actinidia deliciosa)

González¹,

Coque²,

Herrero

1998

Annals of Applied Biology

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I SummaryKiwifruit (Actinidiu deliciosa (Chev.) Liang and Ferguson) is a dioecious species highly pollination dependent, since fruit size is directly correlated with seed number. To evaluate the best pollination system for this species a study of two natural and two artificial pollination systems was carried out and their effect on fruit set and fruit quality was determined. While anemophilous pollination produced few and small fruits, that were not commercially acceptable, high fruit set percentages and good quality fruits were obtained when insects participated in the pollination process. Artificial pollination, performed as hand pollination, improved yield quantity and quality. When environmental conditions impair insect activity this method can be an useful agricultural practice in order to assure a good yield.

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Section: Discussionmentioning

confidence: 77%

“…Similar results have been obtained using other systems. Thus, King & Ferguson (1991) recorded an acceptable fruit yield using a mechanical system with dry pollen, but still lower than that obtained using traditional artificial systems with wet pollen.…”

Section: Discussionmentioning

confidence: 78%

Influence of pollination systems on fruit set and fruit quality in kiwifruit (Actinidia deliciosa)

González¹,

Coque²,

Herrero

1998

Annals of Applied Biology

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“…Based on the above, we recommend using the terminology ‘imbibed pollen’ when pollen isolation is performed in any water-containing solution, to discriminate with the data from naturally dry mature pollen. An interesting development is the use of vacuum devices to efficiently isolate dry pollen from anthers (King and Ferguson 1991 ; patent WO2012/5593A2). It remains to be established, however, whether such devices can also be used to isolate pollen fractions other than mature pollen in a dry manner, without the use of watery solutions and an anther squeezing step.…”

Section: Resultsmentioning

confidence: 99%

The effect of isolation methods of tomato pollen on the results of metabolic profiling

et al. 2019

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IntroductionUntargeted metabolomics is a powerful tool to detect hundreds of metabolites within a given tissue and to compare the metabolite composition of samples in a comprehensive manner. However, with regard to pollen research such comprehensive metabolomics approaches are yet not well developed. To enable isolation of pollen that is tightly enclosed within the anthers of the flower, such as immature pollen, the current pollen isolation protocols require the use of a watery solution. These protocols raise a number of concerns for their suitability in metabolomics analyses, in view of possible metabolic activities in the pollen and contamination with anther metabolites.ObjectivesWe assessed the effect of different sample preparation procedures currently used for pollen isolation for their suitability to perform metabolomics of tomato pollen.MethodsPollen were isolated using different methods and the metabolic profiles were analysed by liquid chromatography–mass spectrometry (LC–MS).ResultsOur results demonstrated that pollen isolation in a watery solution led to (i) rehydration of the pollen grains, inducing marked metabolic changes in flavonoids, phenylpropanoids and amino acids and thus resulting in a metabolite profile that did not reflect the one of mature dry pollen, (ii) hydrolysis of sucrose into glucose and fructose during subsequent metabolite extraction, unless the isolated and rehydrated pollen were lyophilized prior to extraction, and (iii) contamination with anther-specific metabolites, such as alkaloids, thus compromising the metabolic purity of the pollen fraction.ConclusionWe conclude that the current practices used to isolate pollen are suboptimal for metabolomics analyses and provide recommendations on how to improve the pollen isolation protocol, in order to obtain the most reliable metabolic profile from pollen tissue.Electronic supplementary materialThe online version of this article (10.1007/s11306-018-1471-4) contains supplementary material, which is available to authorized users.

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“…Furthermore, a consideration of using a wet pollen‐based approach is that the system will be most effective during the day time. Wetting the pollen stimulates early germination, limiting the window in which the pollen is viable for application requiring timely application of the pollen before it is nonviable (King & Ferguson, ). Kiwifruit pollen germinates best in warmer temperatures, being less effective if applied during cold conditions (Jansson & Warrington, ).…”

Section: Machine Visionmentioning

confidence: 99%

Autonomous pollination of individual kiwifruit flowers: Toward a robotic kiwifruit pollinator

Williams

Nejati

Hussein

et al. 2019

Journal of Field Robotics

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There is an increasing concern that the traditional approach of natural kiwifruit pollination by bees may not be sustainable. The alternatives are currently too costly for most growers due to high labor requirements or inefficient usage of expensive pollen. This paper presents a performance evaluation of a novel kiwifruit pollinating robot designed to provide a more efficient, reliable, and cost‐effective means of producing kiwifruit. The robot comprises a novel air‐assisted sprayer, a machine vision system employing convolution neural networks, and a flower targeting system for efficient and effective application of pollen to individual flowers. We show that this pollination system is capable of individually targeting and pollinating 79.5% of flowers at 3.5 km/hr while using comparable amounts of pollen to commercial Cambrian operators. Furthermore, flowers that were successfully pollinated at 1 km/hr grew into the first robotically pollinated kiwifruit which were comparable in quality to commercially grown kiwifruit. However, the overall fruit set was found to be well below commercial requirements and further work on increasing the overall yield is required.

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Collection and use of dry pollen for pollination of kiwifruit

Cited by 10 publications

References 2 publications

Influence of pollination systems on fruit set and fruit quality in kiwifruit (Actinidia deliciosa)

Influence of pollination systems on fruit set and fruit quality in kiwifruit (Actinidia deliciosa)

The effect of isolation methods of tomato pollen on the results of metabolic profiling

Autonomous pollination of individual kiwifruit flowers: Toward a robotic kiwifruit pollinator

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