This work was developed with the objective of characterizing the variability and genetic structure of matrices and progenies of the Mangaba genebank of Embrapa Tabuleiros Costeiros, to de ne a core collection. From the matrix of seven accessions, 289 individuals were generated, evaluated by nine SSR markers. The characterization of genetic variability was performed from the estimates: Average number of alleles observed (Na = 5.63) and effective (Ne = 2.71); Shannon Information Index (I = 1.04); Heterozygosity observed (Ho = 0.45) and expected (He = 0.51); Fixation index (f = 0.13); Percent polymorphism (%P = 90.47); and, Number of private alleles (Nap = 20). The Analysis of Molecular Variance (AMOVA) identi ed that the largest proportion of genetic variation is present within accessions (92%). The estimates of genetic differentiation (G ST and R ST ) were considered low (< 0.05) to moderate (0.05 to 0.15) magnitude. Multivariate analyses (PCoA, Rogers and Bayesian genetic diversity) did not discriminate the accessions according to origin. The formation of the core collection allowed retaining 94.90% of the identi ed alleles. The accessions present genetic variability to be explored in mangaba conservation and genetic improvement programs.