Collection of allogeneic peripheral blood progenitor cells by two protocols on an apheresis system

Schreiner, Thomas; Wiesneth, Markus; Krug, Ethan; Kalinova, J; Büchele, H; Maccari, Birgit; Erne, Elke; Bischof, Christine; Reys, S De

doi:10.1046/j.1537-2995.1998.38111299056316.x

Cited by 32 publications

(23 citation statements)

References 11 publications

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“…When comparing leukapheresis volumes and components obtained with the two collection strategies, our results are in agreement with data from literature showing a major difference in the harvest volume, which is generally higher when V4.7 is applied [13,17]. Probably, an enrichment of nucleated cells is obtained with V4.7, also in MM patients higher preleukapheresis WBC counts.…”

Section: Discussionsupporting

confidence: 90%

“…Probably, an enrichment of nucleated cells is obtained with V4.7, also in MM patients higher preleukapheresis WBC counts. On the other hand, in our study, the number of platelets collected with V4.7 was significantly lower than reported, either in the PBSC collections from patients with a variety of malignancies or allogeneic donors [12,17,18] or in the MNC collection [19] when compared with V6.0. As a matter of fact, in our data, platelets CE% and RBC contamination was similar in both aphereses system, while hemoglobin was more concentrated in V6.0 harvest.…”

Section: Discussioncontrasting

confidence: 87%

See 1 more Smart Citation

Peripheral blood stem cell collection in multiple myeloma: A retrospective analysis of 6 years leukapheresis activity in 109 patients treated at the Istituto Nazionale dei Tumori of Milan

Coluccia

Montefusco

Tùnesi

et al. 2009

J of Clinical Apheresis

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Double autologous stem cell transplantation is the standard treatment in newly diagnosed multiple myeloma (MM) patients younger than 65 years; therefore, optimization of leukapheresis is crucial. We performed a retrospective analysis of 297 leukaphereses comparing semiautomated (V4.7 in 20% of collections) versus automated (V6.0 in 80%) Caridian (COBE) Spectra versions and analyzing the influence of M-protein on the outcome. Both methods gave comparable collection efficiencies (CE%) (53.4% vs. 55.7% in V6.0 and V4.7, respectively) with similar leukapheresis time and processed volume. Harvest volume was higher in V4.7 (P < 0.0001) with similar contamination of red blood cells (RBCs) (P = 0.77) and platelets (P = 0.09) when compared with V6.0. In patients with higher peripheral white blood cells (WBCs), V6.0 with adjusted harvest volume (<700 mL), achieved similar CD34(+) CE% (P = 0.39) and better enrichment of nucleated cells (P < 0.0,002) but higher RBCs (P < 0.0,001) and platelets contamination (P = 0.001), when compared with a larger cycle volume in patients with lower WBCs. In hard to mobilize patients, CD34(+) CE% was significantly more efficient with V4.7 than V6.0 (P < 0.0,001). CD34(+) CE% was unaffected by serologic M-protein, but platelet CE% was higher in the absence of M-protein (P = 0.0,003), without any reduction in peripheral patients platelets. We, therefore, conclude that in the setting of MM patients with a high WBCs count and/or low percentage of peripheral CD34(+) cells, collections with V4.7 or adjusted cycle volume V6.0 gave comparable result in CD34(+) CE%. RBCs and platelets contamination is higher if low cycle volume is chosen. In hard to mobilize patients, V4.7 is advisable.

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Section: Discussionsupporting

confidence: 90%

Section: Discussioncontrasting

confidence: 87%

Peripheral blood stem cell collection in multiple myeloma: A retrospective analysis of 6 years leukapheresis activity in 109 patients treated at the Istituto Nazionale dei Tumori of Milan

Coluccia

Montefusco

Tùnesi

et al. 2009

J of Clinical Apheresis

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“…The product yields and CE of white blood cells (WBCs), MNCs, and CD34+ cells, and residual PLTs were enumerated. CE calculations were based on the total blood volumes (TBVs) of donors and patients (TBV‐CE) or the cell numbers passed through the instrument (CE1)

\begin{matrix} TBV-CE = number of collected cells \times 100 / \\ preapheresis cell count \times TBV . \end{matrix}

\begin{matrix} TBV-CE = number of collected cells \times 100 / \\ preapheresis cell count \times TBV . \end{matrix}

…”

Section: Methodsmentioning

confidence: 99%

“…The reported advantages of Spectra‐Auto, comparing with other apheresis platforms, are decreased citrate toxicity, less PLT contamination, and feasible CEs 14‐19. However, there are few direct comparisons of Spectra‐MNC and Spectra‐Auto, and uncertainty remains.…”

mentioning

confidence: 99%

Peripheral blood progenitor cell collection by two programs for autologous and allogeneic transplantation

et al. 2013

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“…CD34+ cell collection efficiency [6,7] and recruitment factor [8][9][10] for the various cell populations were calculated as previously described.…”

Section: Laboratory Evaluation Of Peripheral Blood and Leukapheresis mentioning

confidence: 99%

Large volume leukapheresis: Efficacy and safety of processing patient’s total blood volume six times

Bojanić

Dubravčić

Batinić

et al. 2011

Transfusion and Apheresis Science

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Collection of allogeneic peripheral blood progenitor cells by two protocols on an apheresis system

Cited by 32 publications

References 11 publications

Peripheral blood stem cell collection in multiple myeloma: A retrospective analysis of 6 years leukapheresis activity in 109 patients treated at the Istituto Nazionale dei Tumori of Milan

Peripheral blood stem cell collection in multiple myeloma: A retrospective analysis of 6 years leukapheresis activity in 109 patients treated at the Istituto Nazionale dei Tumori of Milan

Peripheral blood progenitor cell collection by two programs for autologous and allogeneic transplantation

Large volume leukapheresis: Efficacy and safety of processing patient’s total blood volume six times

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