Collection of epididymal semen in the tomcat (Felix catus) by stereomicroscope-aided retrograde flushing (SARF) improves sample quality

Monaco, Davide; Rota, Alessandra; Carbonari, Alice; Lillo, Edoardo; Lacalandra, Giovanni Michele; Rizzo, Annalisa

doi:10.1016/j.anireprosci.2023.107388

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2024

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Cited by 5 publications

References 30 publications

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Epididymal sperm characteristics in Brown-throated Sloth (Bradypus variegatus Schinz, 1825)

Pinto,

Nascimento e Silva,

Couto

et al. 2024

Theriogenology Wild

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Epididymal sperm characteristics in Brown-throated Sloth (Bradypus variegatus Schinz, 1825)

Pinto,

Nascimento e Silva,

Couto

et al. 2024

Theriogenology Wild

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Oviductin sets the species-specificity of the mammalian zona pellucida

de la Fuente,

Maroto,

Cajas

et al. 2024

Preprint

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The zona pellucida (ZP) is vital for species-specific fertilization as this barrier mediates sperm-oocyte binding. Here, we determined whether sperm from distant mammalian orders (Carnivora, Primates, and Rodentia) could penetrate bovine oocytes by examining the role of bovine oviductal fluid and species-specific oviductal glycoprotein (OVGP1 or oviductin) from bovine, murine, or human sources in modulating the species specificity of bovine and murine oocytes. Sperm from all the species were found to penetrate intact bovine ovarian oocytes to form hybrid embryos. However, contact with oviductal fluid or bovine, murine, or human OVGP1, conferred the ZP species specificity, allowing only the penetration of the corresponding sperm regardless of the ZP’s origin. Glycolytic and microstructural analyses revealed that OVGP1 covers the pores present in the ZP and that OVGP1 glycosylation determines sperm specificity. This suggests specific fertilization capacity is acquired in the oviduct through the ZP’s incorporation of specific oviductin.TeaserThe oocyte zona pellucida needs to interact with an oviduct protein called OVGP1 to ensure that only sperm from the same species can fertilize the egg.Abstract FigureModel

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Enhancing felid conservation: Exploring the impact of in vitro culture media on domestic cat blastocyst production

Munoz‐Maceda,

Priego‐Gonzalez,

Núñez‐Puente

et al. 2024

Reprod Domestic Animals

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This study investigated the optimization of assisted reproductive techniques for wild felid conservation, focusing on in vitro procedures using the domestic cat as a model species. The research evaluated the impact of three different in vitro culture media on blastocyst formation. Oocytes and spermatozoa were collected and processed, followed by in vitro fertilization and culture. Results returned a similar blastocyst rate (ANOVA, p > .05), over 16% across all groups. While demonstrating the potential of these techniques, further investigations are warranted to evaluate embryo quality to refine optimal protocols and their applicability in felid conservation efforts.

show abstract

Collection of epididymal semen in the tomcat (Felix catus) by stereomicroscope-aided retrograde flushing (SARF) improves sample quality

Cited by 5 publications

References 30 publications

Epididymal sperm characteristics in Brown-throated Sloth (Bradypus variegatus Schinz, 1825)

Epididymal sperm characteristics in Brown-throated Sloth (Bradypus variegatus Schinz, 1825)

Oviductin sets the species-specificity of the mammalian zona pellucida

Enhancing felid conservation: Exploring the impact of in vitro culture media on domestic cat blastocyst production

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