Collection of gas-chromatographic fractions for identification by infrared spectrophotometry

Abstract

ALTHOUGH many types of apparatus are available commercially and many more have been proposed for the collection of gas-chromatographic fractions, they are mainly useful only on a milligram scale, e.g., Haslam, Jeffs and Willisl have decribed methods that will handle down to 1 to 2 p1 of liquids. However, when 0-2 mg or less of material is to be collected, difficulties are encountered, the main one being that, with the rapid flow of carrier gas from the outlet of the chromatograph,

Determination of the Carbon Skeleton and Other Structural Features of Organic Compounds by Gas Chromatography.

“…Quantitative analysis may be based on the graded response. In several experiments, areas under peaks obtained by injecting mixtures of known composition have been within 5 to 10% of the expected amount. Although the accuracy of this result leaves much to be desired, it can undoubtedly be improved for specific analyses.…”

“…of heptane, the expected major product. This amount of substance is sufficient for an infrared (1) or mass spectrum analysis (5).…”