Collection of new diversity of wild and cultivated bananas (Musa spp.) in the Autonomous Region of Bougainville, Papua New Guinea

Sardos, Julie; Christelová, Pavla; Čížková, Jana; Paofa, J.; Sachter-Smith, G.; Janssens, Steven; Rauka, G. B.; Ruas, Max; Daniells, J. W.; Doležel, Jaroslav; Roux, Nicolás

doi:10.1007/s10722-018-0690-x

Cited by 21 publications

(14 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was the case notably for the banana collection conserved at the USDA-ARS Tropical Agriculture Research Station in Puerto Rico, which successfully characterized its germplasm (Irish et al 2014). The Musa Genotyping Centre also allowed the 'real-time' characterization of the germplasm collected during collecting missions in Indonesia in 2012 and 2013 (Christelová et al 2017) and recently in the Autonomous Region of Bougainville (Papua New Guinea) (Sardos et al 2018). The platform therefore has become a powerful and affordable way to characterize banana germplasm globally and is an essential tool for and efficient conservation and use of banana germplasm.…”

Section: Molecular Characterizationmentioning

confidence: 99%

Safeguarding and using global banana diversity: a holistic approach

houwe¹,

Chase

Sardos

et al. 2020

CABI Agric Biosci

View full text Add to dashboard Cite

The CGIAR genebank International Musa Germplasm Transit Centre (ITC) currently holds 1617 banana accessions from 38 countries as an in vitro collection, backed-up by a cryopreserved collection to safeguard global Musa diversity in perpetuity. The ITC also serves as a vital safety backup and transit centre for national banana genebanks and ensures that germplasm is clean of pests and diseases and freely available under the International Treaty on Plant Genetic Resources for Food and Agriculture. In more than 35 years of activity, the ITC has distributed over 18,000 banana accession samples to researchers and farmers in 113 countries. Ex situ conservation of vegetatively-propagated crops such as banana poses very particular challenges. Maintaining the ITC genebank is labor intense and costly. Efficiencies are sought through research and development of techniques on detecting viruses, the genetic integrity of accessions, and on innovative means of safeguarding banana diversity, such as conserving populations of wild species by seed banking. Although the conservation of global banana diversity is the main objective of the ITC, significant value comes from its holistic approach to better understand and promote its germplasm through numerous research activities and resources. Techniques for morphological and molecular characterization serve to identify and describe the collection, while also determining what gaps should be filled by collecting missions with national partners. The evaluation of desirable agronomic traits inherent in Musa spp. are investigated by a high-throughput phenotyping platform, which helps breeding programs to select cultivars resistant or tolerant to biotic and abiotic stresses. Genomic and bioinformatic studies of several banana wild relatives greatly enhance our understanding of Musa genetic diversity, links to important phenotypic traits and bring new methods for management of the collection. Collectively, these research activities produce enormous amounts of data that require curation and dissemination to the public. The two information systems at the ITC, Musa Genebank Management System and the Musa Germplasm Information System, serve to manage the genebank activities and to make public germplasm-related data for over 30 banana collections worldwide, respectively. By implementing the 10-year workplan set out in the Global Strategy for the Conservation and Use of Musa Genetic Resources, the network MusaNet supports Musa researchers and stakeholders, including the ITC, and most importantly, links to the world’s banana-producing countries via three regional banana networks.

show abstract

Section: Molecular Characterizationmentioning

confidence: 99%

Safeguarding and using global banana diversity: a holistic approach

houwe¹,

Chase

Sardos

et al. 2020

CABI Agric Biosci

View full text Add to dashboard Cite

show abstract

“…Collectors must access bunches before they are consumed and seeds are dispersed by birds and mammals [ 82 , 83 , 84 ]. Humans also harvest wild bananas for food, construction and artistry [ 37 , 85 ]. It is therefore important to be able to identify fruits that contain seeds that are mature enough to be desiccation tolerant, without knowing flowering times, whilst they may not have yet attracted frugivores.…”

Section: Discussionmentioning

confidence: 99%

“…Sixteen wild Musa taxa occur in PNG [33]. Several collecting missions have been made in PNG to characterise and collect both cultivar and CWR germplasm [34][35][36][37][38]. These include seed collections, two of which we evaluate here.…”

Section: Introductionmentioning

confidence: 99%

Challenges for Ex Situ Conservation of Wild Bananas: Seeds Collected in Papua New Guinea Have Variable Levels of Desiccation Tolerance

Kallow

Longin

Sleziak³

et al. 2020

Plants

View full text Add to dashboard Cite

Ex situ seed conservation of banana crop wild relatives (Musa spp. L.), is constrained by critical knowledge gaps in their storage and germination behaviour. Additionally, challenges in collecting seeds from wild populations impact the quality of seed collections. It is, therefore, crucial to evaluate the viability of seeds from such collecting missions in order to improve the value of future seed collections. We evaluate the seed viability of 37 accessions of seven Musa species, collected from wild populations in Papua New Guinea, during two collecting missions. Seeds from one mission had already been stored in conventional storage (dried for four months at 15% relative humidity, 20 °C and stored for two months at 15% relative humdity, −20 °C), so a post-storage test was carried out. Seeds from the second mission were assessed freshly extracted and following desiccation. We used embryo rescue techniques to overcome the barrier of germinating in vivo Musa seeds. Seeds from the first mission had low viability (19 ± 27% mean and standard deviation) after storage for two months at 15% relative humidity and −20 °C. Musa balbisiana Colla seeds had significantly higher post-storage germination than other species (p < 0.01). Desiccation reduced germination of the seeds from the second collecting mission, from 84 ± 22% (at 16.7 ± 2.4% moisture content) to 36 ± 30% (at 2.4 ± 0.8% moisture content). There was considerable variation between and (to a lesser extent) within accessions, a proportion of individual seeds of all but one species (Musa ingens N.W.Simmonds) survived desiccation and sub-zero temperature storage. We identified that seeds from the basal end of the infructescence were less likely to be viable after storage (p < 0.001); and made morphological observations that identify seeds and infructescences with higher viability in relation to their developmental maturity. We highlight the need for research into seed eco-physiology of crop wild relatives in order to improve future collecting missions.

show abstract

“…A total of 591 accessions, with coherent classification and genotyping data, were appointed as a core set (CS) to act as a source with which subsequently analyzed material can be compared. In another study, Sardos et al [ 110 ] genotyped wild and cultivated accessions collected in the Bougainville region (AROB), Papua New Guinea, using the same 19 SSR loci, and subsequently aligned the profiles to the CS database. Joint analysis of CS and AROB data enabled confirmation of the classification of AROB accessions.…”

Section: Genetic Diversitymentioning

confidence: 99%

Recent Large-Scale Genotyping and Phenotyping of Plant Genetic Resources of Vegetatively Propagated Crops

Nybom

Lācis

2021

Plants

View full text Add to dashboard Cite

Several recent national and international projects have focused on large-scale genotyping of plant genetic resources in vegetatively propagated crops like fruit and berries, potatoes and woody ornamentals. The primary goal is usually to identify true-to-type plant material, detect possible synonyms, and investigate genetic diversity and relatedness among accessions. A secondary goal may be to create sustainable databases that can be utilized in research and breeding for several years ahead. Commonly applied DNA markers (like microsatellite DNA and SNPs) and next-generation sequencing each have their pros and cons for these purposes. Methods for large-scale phenotyping have lagged behind, which is unfortunate since many commercially important traits (yield, growth habit, storability, and disease resistance) are difficult to score. Nevertheless, the analysis of gene action and development of robust DNA markers depends on environmentally controlled screening of very large sets of plant material. Although more time-consuming, co-operative projects with broad-scale data collection are likely to produce more reliable results. In this review, we will describe some of the approaches taken in genotyping and/or phenotyping projects concerning a wide variety of vegetatively propagated crops.

show abstract

Collection of new diversity of wild and cultivated bananas (Musa spp.) in the Autonomous Region of Bougainville, Papua New Guinea

Cited by 21 publications

References 38 publications

Safeguarding and using global banana diversity: a holistic approach

Safeguarding and using global banana diversity: a holistic approach

Challenges for Ex Situ Conservation of Wild Bananas: Seeds Collected in Papua New Guinea Have Variable Levels of Desiccation Tolerance

Recent Large-Scale Genotyping and Phenotyping of Plant Genetic Resources of Vegetatively Propagated Crops

Contact Info

Product

Resources

About