Collection of red blood cell units by apheresis

Moog, Rainer

doi:10.1016/j.transci.2013.02.008

Cited by 4 publications

(2 citation statements)

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“…Synaptosome samples were prepared as previously described [3][4][5]. Human platelets and RBCs were obtained by apheresis from the Stanford Blood Center using established methods [18,19]. Cryopreserved samples were thawed only once.…”

Section: Samplesmentioning

confidence: 99%

Mass‐tag barcoding for multiplexed analysis of human synaptosomes and other anuclear events

et al. 2021

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Mass-tag cell barcoding has increased the throughput, multiplexing, and robustness of multiple cytometry approaches. Previously, we adapted mass cytometry for cells to analyze synaptosome preparations (mass synaptometry or SynTOF), extending mass cytometry to these smaller, anuclear particles. To improve throughput and individual event resolution, we report here the application of palladium-based barcoding in human synaptosomes. Up to 20 individual samples, each with a unique combinatorial barcode, were pooled for labeling with an antibody cocktail. Our synaptosome protocol used six palladium-based barcoding reagents, and in combination with sequential gating increased the identification of presynaptic events approximately fourfold. These same parameters also efficiently resolved two other anuclear particles: human red blood cells and platelets. The addition of palladium-based mass-tag barcoding to our approach improves mass cytometry of synaptic particles.

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Section: Samplesmentioning

confidence: 99%

Mass‐tag barcoding for multiplexed analysis of human synaptosomes and other anuclear events

et al. 2021

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“…Collections of 2RBC units are performed with the MCS+, the Trima and the ALYX. With all systems, predefined absolute RBC volumes with small variations are obtained . With 2RBC procedures, cell volumes of 360, 400 or 420 ml can be collected that are then manually divided into two single‐dose units.…”

Section: Practical Aspects Of 2rbc Apheresis and Quality Of Automaticmentioning

confidence: 99%

Red cell apheresis: pros and cons

Infanti

2017

ISBT Science Series

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The collection of red blood cells (RBC) by apheresis, first developed in the 1970's for therapeutic use, for many years was not considered suitable for obtaining blood components for transfusion because of concerns on donor safety and elevated costs compared to whole blood (WB) donation. Single-dose RBC apheresis as a part of multicomponent collections and double-dose RBC (2RBC) apheresis for autologous and allogeneic transfusions were approved by the FDA between 1995 and 1997, and in Europe starting from 2000. The widespread implementation of these procedures followed mainly to address blood supply issues, as large numbers of blood components can be rapidly obtained. However, this potential advantage has been only partially confirmed in real practice, with striking regional differences. Limitations of 2RBC collections regarding both blood supply and donor safety have emerged. In particular, donor motivation, iron deficiency and risk of anaemia are issues that need to be addressed. On the other side, 2RBC apheresis represents a valuable alternative to WB collection for autologous or dedicated donations, can reduce the risk of alloimmunization in chronically transfused patients and can facilitate transfusion support in specific cases (i.e. patients with multiple alloantibodies or rare blood groups). Reducing patient exposure to different donors can also be relevant for preventing the transmission of blood-borne infections.

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