Collective and individual migration following the epithelial–mesenchymal transition

Wong, Ian Y.; Javaid, Sarah; Wong, Elisabeth; Perk, Sinem; Haber, Daniel A.; Toner, Mehmet; Irimia, Daniel

doi:10.1038/nmat4062

Cited by 166 publications

(171 citation statements)

References 46 publications

Supporting

Mentioning

165

Contrasting

Unclassified

Order By: Relevance

“…Each pathway when separately activated, increased EMT, migration and invasion, whereas a dual activation reduced this effect. However, EMT and migration and invasion are well related [34, 35]. Cancer cells undergoing metastasis frequently show the phenomenon of EMT.…”

Section: Discussionmentioning

confidence: 99%

Co-Activation of TGFβ and Wnt Signalling Pathways Abrogates EMT in Ovarian Cancer Cells

Mitra¹,

Roy²

2017

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: The aggressive property of ovarian cancer (OC) in terms of epithelial-mesenchymal transition (EMT), proliferation and metastasis are of major concern. Different growth factors including TGFβ are associated with regulating these molecular events but the underlying mechanisms remain unclear. The aim of this report is to decipher the regulation of EMT by co-activation of TGFβ and Wnt signalling cascades in gaining malignancy. Methods: The expression of the different components of signalling events were analyzed by QPCR, Western blot, Immunofluorescence microscopy and flow cytometry. β-catenin promoter activity was checked by luciferase assay. Results: We observed reduced EMT in ovarian cancer cells upon co-activation with TGFβ1 and LiCl as shown by the expressions of epithelial/mesenchymal markers and the EMT promoting factor, Snail1, accompanied by decrease in the invasion and migration of the cells compared to individual pathway activation. A detailed study of the mechanism suggested reduction in the β-catenin and p-GSK3b (Ser 9) levels to be the driving cause of this phenomenon, which was reversed upon co-activation with higher concentrations of LiCl. Conclusions: Therefore, tumourigenesis might be affected by the concentration of ligand/ growth factors for the respective signalling pathways activated in the tumour microenvironment and interaction between them might alter tumourigenesis.

show abstract

Section: Discussionmentioning

confidence: 99%

Co-Activation of TGFβ and Wnt Signalling Pathways Abrogates EMT in Ovarian Cancer Cells

Mitra¹,

Roy²

2017

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…Vimentin is a cytoskeletal protein that is known to be up-regulated in highly motile cancer cells. 20 Fast- and slow-moving GBM157 clones were then randomly identified from a ∼16 h time-lapse experiment on our chip (Figure 1e) and subsequently transported into the microchannels of the NEP component using optical tweezers. The juxtaposing array of microchannels was filled with molecular beacons (MBs) against vimentin .…”

Section: Patient-derived Gscs Exhibition Of Distinct and Heterogeneoumentioning

confidence: 99%

On-Chip Clonal Analysis of Glioma-Stem-Cell Motility and Therapy Resistance

et al. 2016

View full text Add to dashboard Cite

Enhanced glioma-stem-cell (GSC) motility and therapy resistance are considered to play key roles in tumor cell dissemination and recurrence. As such, a better understanding of the mechanisms by which these cells disseminate and withstand therapy could lead to more efficacious treatments. Here, we introduce a novel micro-/nanotechnology-enabled chip platform for performing live-cell interrogation of patient-derived GSCs with single-clone resolution. On-chip analysis revealed marked intertumoral differences (>10-fold) in single-clone motility profiles between two populations of GSCs, which correlated well with results from tumor-xenograft experiments and gene-expression analyses. Further chip-based examination of the more-aggressive GSC population revealed pronounced interclonal variations in motility capabilities (up to ∼4-fold) as well as gene-expression profiles at the single-cell level. Chip-supported therapy resistance studies with a chemotherapeutic agent (i.e., temozolomide) and an oligo RNA (anti-miR363) revealed a subpopulation of CD44-high GSCs with strong antiapoptotic behavior as well as enhanced motility capabilities. The living-cell-interrogation chip platform described herein enables thorough and large-scale live monitoring of heterogeneous cancer-cell populations with single-cell resolution, which is not achievable by any other existing technology and thus has the potential to provide new insights into the cellular and molecular mechanisms modulating glioma-stem-cell dissemination and therapy resistance.

show abstract

“…Chemie von Zell-Zell-Wechselwirkungen wurden kultivierte Zellen bei ihrem Eintritt in verschiedene EMT-Phasen untersucht; [82] Zellen unterschiedlicher Phasen zeigten unterschiedliche Empfindlichkeiten gegenüber Chemotherapeutika. Dieser Ansatz wurde jedoch noch nicht an Patienten-CTCs erprobt.…”

Section: Methodsunclassified

Profilierung zirkulierender Tumorzellen mit Apparaturen und Materialien der nächsten Generation

Green

Safaei²,

Mepham

et al. 2015

Angewandte Chemie

View full text Add to dashboard Cite

In den letzten zehn Jahren gab es wichtige Fortschritte bei der Erfassung seltener zirkulierender Tumorzellen (CTCs) aus dem Blut von Krebspatienten als eine entscheidende Voraussetzung für die nichtinvasive Tumorprofilierung. Diese Fortschritte haben zudem neue Einblicke in die Materialchemie und die Mikrofluidik erbracht und ermöglichten die Erfassung und Auszählung von CTCs mit beispielloser Empfindlichkeit. Allerdings wurde auch immer klarer, dass einfaches Isolieren und Auszählen von Tumorzellen, die in den Kreislauf gelangt waren, nicht die benötigten Informationen lieferte, um unser Verständnis der Biologie dieser seltenen Zellen zu vertiefen oder um sie sich in der Therapie besser zunutze zu machen. Mithilfe von Apparaturen und Materialien der nächsten Generation mit maßgeschneiderten physikalischen und chemischen Eigenschaften ist man inzwischen in der Lage, mehr Informationen aus CTCs zu gewinnen. In diesem Kurzaufsatz werden die Arbeiten der letzten zehn Jahre in diesem Gebiet diskutiert, wobei insbesondere die bahnbrechenden Studien der letzten fünf Jahre Berücksichtigung finden, die den Blick über das einfache Isolieren von CTCs hinaus auf Ansätze gerichtet haben, die eine tiefergehende Analyse ermöglichen.

show abstract

Collective and individual migration following the epithelial–mesenchymal transition

Cited by 166 publications

References 46 publications

Co-Activation of TGFβ and Wnt Signalling Pathways Abrogates EMT in Ovarian Cancer Cells

Co-Activation of TGFβ and Wnt Signalling Pathways Abrogates EMT in Ovarian Cancer Cells

On-Chip Clonal Analysis of Glioma-Stem-Cell Motility and Therapy Resistance

Profilierung zirkulierender Tumorzellen mit Apparaturen und Materialien der nächsten Generation

Contact Info

Product

Resources

About