Deep evolutionary time (DET) -emergence of life over immense time scales -is a threshold concept in biology. While understanding DET might help learners and citizens contemplate their actions in the context of human existence, especially in combination with experiencing evoked affective responses, interpreting temporal aspects of DET is demanding. DET is communicated through various visualizations that include static two-dimensional representations (e.g., phylograms), interactive animations (e.g., evolution timelines), as well as touch-based interfaces (e.g., dynamic representations of phylogenetic trees). Given the importance of DET as fundamental scientific knowledge of potential societal application, there is a need for educational research on students' interpretation of visually communicated DET. This thesis explores students' interpretation of different forms of visualized DET along a continuum of interactivity, ranging from non-interactive to highly interactive visualizations.The research aim is four-fold, and probes how students interpret DET visualizations in terms of: i) temporal aspects, ii) communicated evolutionary concepts, iii) degree of visualization interactivity, and iv) generated affective responses.The work comprises four studies, which as a collective, adopt exploratory and multi-method fixed and flexible designs. A total of 505 upper secondary, college bridging-year, and university students participated. Data were collected from written and online questionnaires, task-based questions, and semi-structured interviews. Data analysis was qualitative and quantitative, and incorporated deductive and inductive approaches.In analysing students' interpretation of static two-dimensional DET visualizations such as phylograms (Paper I), an instrument for measuring knowledge about the visual representation of deep evolutionary time (DET-Vis) was developed. Emergence of a unidimensional construct during validation represents knowledge about the visual communication of DET. Inspection of item performance suggests that interpreting visualized DET requires both procedural and declarative knowledge. In moving across the continuum from static to more dynamic DET visualizations, analysis of students' interpretation of an animation communicating hominin evolution (Paper II) revealed five temporal aspects influencing interpretation, namely: events at specific times, relative order, concurrent events, time intervals, and time interval durations. A further shift across the continuum involved analysing students' interpretation of a touch-based DET visualization of a three-dimensional phylogenetic tree (DeepTree) (Paper III). Finger-based zooming was associated with movement within the tree itself (spatially orientated), or as movement in iv time (time orientated), respectively, and related to identified misinterpretations. Further analysis showed that interpreting DeepTree evoked the epistemic affective responses of awe, curiosity, surprise, and confusion (Paper IV). Affective responses were expressed in relation to ...