Colligated multiplexing approach to expanding the field of view in the recording process of digital holographic microscopy

Wu, Yongli; Yang, Yong; Zhai, Hongchen; Ma, Zhixin; Qi, Gai; Deng, Lijun

doi:10.7498/aps.62.084203

Acta Phys. Sin.

2013

DOI: 10.7498/aps.62.084203

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Colligated multiplexing approach to expanding the field of view in the recording process of digital holographic microscopy

Yongli Wu¹,

Yong Yang²,

Hongchen Zhai³

et al.

Abstract: We present a method to expand the record field of view in the recording process of digital holographic microscopy systems without loss of resolution. A series of incoherent sub-holograms covering different regions of sample can be recorded in a single frame of the CCD synchronously based on wavelength and polarization multiplexing. The reconstructed images can be obtained with information about different parts of the sample. Thus, the synthetic reconstructed image with a wider field can be achieved by image st… Show more

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Cited by 2 publications

References 12 publications

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Study on extending the depth of field in reconstructed image for a micro digital hologram

Yang¹,

Wu²,

Ying-Chun³

et al. 2015

Acta Phys. Sin.

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Digital micro holography offers an in-situ, non-contact and three-dimensional way to explore the microscopic world. However, as it is difficult to focalize the whole object in one single reconstructed image, the application of digital micro holography to cases with a large longitudinal object volume is limited by the microscopes depth of field. By extending the depth of field in reconstructed micro holograms in the wavelet domain, this paper fully takes advantage of numerical reconstruction algorithms to solve this problem. First, a recorded hologram is rebuilt using the wavelet transform approach by setting up an appropriate longitudinal interval to obtain a series of reconstructed hologram planes. Then each plane is decomposed with wavelet into its sub-images of both high and low frequencies. Furthermore, the local variance of the maximum intensity gradients of the high- and low-frequency coefficients is calculated and utilized as the focus criterion. Finally, the image planes are fused into a single one with the depth of field extended to a large extent. The feasibility and robustness of this reconstruction procedure for both continuum and particle fields are investigated. One of the demonstrations is made in an experiment of a tilted continuum:carbon fiber. It is different from most of the previous applications where the interrogated is the particles and where the area involved is parallel to the CCD. The carbon fiber gets successfully reconstructed in three dimensions, and the measurement errors of its diameter are presented together with the reconstruction distances. Another is an experiment of a dispersed particle field:micro transparent particles are generated by an ultrasonic atomizer, for which the reconstruction procedure achieves an extended depth of field. In addition, a numerical model based on generalized Lorenz-Mie theory is used to simulate the holograms of both opaque and transparent particles of 1-15 m in diameter. Variations of the longitudinal location errors with the Fraunhofer number are analyzed, and comparisons are made between the results of opaque and transparent particles. Both the experimental and simulation outcomes show that this reconstruction procedure is a reliable one to acquire an extended-depth-of-field hologram for both the continuum and the dispersed particle fields, and then to accurately measure the objects.

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Study on extending the depth of field in reconstructed image for a micro digital hologram

Yang¹,

Wu²,

Ying-Chun³

et al. 2015

Acta Phys. Sin.

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Fresnel incoherent digital holography with large field-of-view

Tang¹,

Wu²,

Rui-Huan³

et al. 2019

Acta Phys. Sin.

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Incoherent digital holography (IDH) is a recently proposed technique to record three-dimensional (3D) information about the object under incoherent illumination, which breaks the limitation that the holographic recording must be illuminated by coherent light sources and thus makes it usable in white-light and fluorescence illuminating circumstance. In particular, the fresnel incoherent correlation holography (FINCH) is an exemplary method which improves the imaging resolution power and efficiency of incoherent digital holography, and it can obtain 3D distribution of objects swiftly without scanning and moving. However, compared with the conventional optical holography, the FINCH system has a very small field-of-view due to the limitation of the pixel number and size of spatial light modulator (SLM). Therefore, expanding the recording field-of-view of FINCH system is very significant for the application of IDH. In the FINCH, the SLM is used as a diffractive beam splitter so that each spherical beam, originating from each object point, is split into two spherical beams with two different curve radii. Then the interference fringes between the two beams are recorded by CCD. In this paper, the field-of-view angle recorded by the SLM is proposed and analyzed based on the physical and numerical principles of the FINCH system. The field-of-view of imaging system is improved by increasing the effective diameter of SLM through moving the center of the dual-lens optical axis mounted on the SLM to the edge in different directions respectively. An optical setup of reflection mode is constructed to verify the theoretical analysis of this study, and the sub-holograms in different field-of-views are obtained by CCD through changing the masks displayed on the SLM sequentially. Then, the complex holograms in different field-of-views are obtained by using the three-step phase-shifting method, and the reconstructed images are acquired respectively through the angular spectrum method (ASM) by using a computer. Finally, the large field-of-view image is obtained by stitching the reconstructed images in each field-of-view by utilizing the matlab program. The experimental results show that the efficient recording field-of-view of SLM can be increased by 2.77 times with our proposed method. Accordingly, the recording field-of-view of the system is improved significantly. The recording field-of-view of the FINCH system will increase further if the center of the dual-lens optical axis continues to move toward the edge. Therefore, this study provides an important support for the further application of high resolution microscopic imaging with large field-of-view.

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Colligated multiplexing approach to expanding the field of view in the recording process of digital holographic microscopy

Cited by 2 publications

References 12 publications

Study on extending the depth of field in reconstructed image for a micro digital hologram

Study on extending the depth of field in reconstructed image for a micro digital hologram

Fresnel incoherent digital holography with large field-of-view

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