Collision-Induced Dissociation of Branched Oligosaccharide Ions with Analysis and Calculation of Relative Dissociation Thresholds

Penn, Sharron G.; Cancilla, Mark T.; Lebrilla, Carlito B.

doi:10.1021/ac960155i

Cited by 85 publications

(78 citation statements)

References 38 publications

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“…At 50% full power, the sum of all other fragments together accounts for more than 70% to the total ion for DFpLNH II and DFLNnH. Our data support the previous report where the oligosaccharides containing fucoses were particularly labile, and losing fucosyl residue required lower energy [23,24]. Fortunately, increasing the laser power resulted in more fragment ions and simultaneously decreased matrix ions.…”

Section: Influence Of the Laser Power On Isd Of Oligosaccharidessupporting

confidence: 89%

Structural Characterization of Neutral Oligosaccharides by Laser-Enhanced In-Source Decay of MALDI-FTICR MS

Yang

Song

et al. 2011

J. Am. Soc. Mass Spectrom.

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MALDI in-source decay (ISD) technique described to date has proven to be a convenient and rapid method for sequencing purified peptides and proteins. However, the general ISD still can not produce adequate fragments for the detailed structural elucidation of oligosaccharides. In this study, an efficient and practical method termed the laser-enhanced ISD (LEISD) technique of MALDI-FTICR MS allows highly reliable and abundant fragmentation of the neutral oligosaccharides, which was attributed to the ultrahigh irradiation laser of mJ level. The yield of ISD fragmentation was evaluated under different laser powers for 7 neutral oligosaccharides using DHB as matrix. Better quality ISD spectra including fragment ions in low-mass region were obtained at higher laser power. Results from the LEISD of oligosaccharides demonstrated that a significantly better signal-to-noise ratio (S/N) and more structural information could be obtained in comparison to the conventional CID. It was also suggested that the valuable A ions derived from cross-ring cleavage of the linear oligosaccharides allowed the distinction among α(1→4)-, α(1→6)-, β(1→4)-, and β(1→3)-linked isobaric structures according to fragment types and intensities. In addition, ideal fragmentation ions observed by LEISD method facilitated the determination of the sequences and branched points of complex oligosaccharides from human milk.

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Section: Influence Of the Laser Power On Isd Of Oligosaccharidessupporting

confidence: 89%

Structural Characterization of Neutral Oligosaccharides by Laser-Enhanced In-Source Decay of MALDI-FTICR MS

Yang

Song

et al. 2011

J. Am. Soc. Mass Spectrom.

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“…In order to confirm the structures proposed for the 4 can be attributed to the loss of water with the formation of ion at m/z 551 from the precursor ion, followed by successive losses of one and two xylosyl residues yielding the fragment ions at m/z 419 and 287, respectively. Loss of water was previously observed in the CID spectra of oligosaccharides 19,30,31 and shown to occur at the reducing end. 32 No Z-type or X-type fragment ions were identified in any of the spectra.…”

Section: Electrospray Ionisationmentioning

confidence: 66%

Structural characterisation of underivatised olive pulp xylo‐oligosaccharides by mass spectrometry using matrix‐assisted laser desorption/ionisation and electrospray ionisation

Reis

Coimbra

Domingues

et al. 2002

Rapid Comm Mass Spectrometry

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Purified olive pulp glucuronoxylans, with a Xyl/GlcA ratio of 7:1, were subjected to mild acid hydrolysis and the mixture of oligosaccharides obtained was fractionated by size exclusion chromatography. One elution fraction representative of low molecular weight oligosaccharides was analysed by mass spectrometry using matrix-assisted laser desorption/ionisation (MALDI) and electrospray ionisation (ESI) as ionisation methods, in the positive mode. Both types of spectra showed cationised molecules [M + Na](+) of xylo-oligosaccharides in a range below m/z 1,000. The xylo-oligosaccharide structures identified were series of neutral oligosaccharides of xylose (Xyl(n), n = 3-7), of acidic oligosaccharides substituted by one glucuronic acid (Xyl(n)GlcA, n = 3-5) and by two glucuronic acid residues (Xyl(n)GlcA(2), n = 2 and 3), and also of acidic oligosaccharides substituted with one 4-O-methylglucuronic acid residue (Xyl(n)meGlcA, n = 2-4). The proposed structures were confirmed by tandem mass (MS/MS) spectra obtained using collision induced dissociation of the molecular ions. Fragmentation of cationised adducts of neutral Xyl(n) yielded C- and A-type fragments, while ammonium adducts mainly yielded B-type fragments. The fragmentation of the sodium adducts of acidic oligosaccharides (Xyl(n)meGlcA, Xyl(n)GlcA) resulted in the loss of the substituting residue (GlcA or meGlcA) as the predominant fragment, while the corresponding ammonium adducts yielded B-type fragments.

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“…been reported. 29 This indicates that the linear hexamer is not identical with the hexamer present in the enzyme digest of AX 70-80%.…”

Section: Electrospray Ms/ms Analysesmentioning

confidence: 97%

Characterization of plant oligosaccharides by matrix‐assisted laser desorption/ionization and electrospray mass spectrometry

Fernández

Obel²,

Scheller³

et al. 2003

J. Mass Spectrom.

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Structural characterization of arabinoxylans from wheat by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and electrospray ionization (ESI) mass spectrometry using a Q-TOF mass analyser (ESI-Q-TOF) or an ion trap (IT) mass analyser is presented. An arabinoxylan sample digested with endoxylanase A was analysed using MALDI-TOF mass spectrometry (MS), resulting in the identification of molecular ions for structures with up to 22 monosaccharide residues. As the two-component monosaccharides xylose and arabinose are isobaric, structures differing in the number of arabinose branching residues were indistinguishable based on molecular mass and also fragmentation pattern upon collision-induced dissociation (CID). Permethylation followed by ESI-CID analyses using ITMS was performed to obtain structural information regarding the number of arabinose branching residues and their spatial arrangement along the xylose backbone. Analysis of the signal corresponding to an oligomer with six monosaccharide residues showed the presence of at least four isomeric structures differing in degree of branching and position of the branched residue relative to the cleavage site of the enzyme. This is the first demonstration of the use of ESI-ITMS for the structural characterization of arabinoxylan mixtures.

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Collision-Induced Dissociation of Branched Oligosaccharide Ions with Analysis and Calculation of Relative Dissociation Thresholds

Cited by 85 publications

References 38 publications

Structural Characterization of Neutral Oligosaccharides by Laser-Enhanced In-Source Decay of MALDI-FTICR MS

Structural Characterization of Neutral Oligosaccharides by Laser-Enhanced In-Source Decay of MALDI-FTICR MS

Structural characterisation of underivatised olive pulp xylo‐oligosaccharides by mass spectrometry using matrix‐assisted laser desorption/ionisation and electrospray ionisation

Characterization of plant oligosaccharides by matrix‐assisted laser desorption/ionization and electrospray mass spectrometry

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