Colloidal and physicochemical characterization of proteincontaining poly(lactide-co-glycolide) (PLGA) microspheres before and after drying

Progress in many therapies, in particular in the therapies based on peptides, proteins and nucleic acids used as bioactive compounds, strongly depends on development of appropriate carriers which would be suitable for controlled delivery of the intact abovementioned compounds to required tissues, cells and intracellular compartments. This review presents last ten years' achievements and problems in development and application of synthetic polymer nanoparticulate carriers for oral, pulmonary and nasal delivery routes of oligopeptides and proteins. Whereas some traditional synthetic polymer carriers are only briefly recalled the main attention is concentrated on nanoparticles produced from functional copolymers mostly with hydroxyl, carboxyl and amino groups, suitable for immobilization of targeting moieties and for assuring prolonged circulation of nanoparticles in blood. Formulations of various nanoparticulate systems are described, including solid particles, polymer micelles, nanovesicles and nanogels, especially systems allowing drug release induced by external stimuli. Discussed are properties of these species, in particular stability in buffers and models of body fluids, loading with drugs and with drug models, drug release processes and results of biological studies. There are also discussed systems for gene delivery with special attention devoted to polymers suitable for compacting nucleic acids into nanoparticles as well as the relations between chemical structure of polymer carriers and ability of the latter for crossing cell membranes and for endosomal escape.

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“…Elaissari and coworkers did show that this subject is of significant importance for design of nanoparticulate drug delivery systems [115].…”

Section: Discussionmentioning

confidence: 99%

Progress in Nanoparticulate Systems for Peptide, Proteins and Nucleic Acid Drug Delivery

Słomkowski¹,

Gosecki

2011

CPB

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“…The MPS‐PLGA was prepared using a modified double‐emulsion method . The water‐in‐oil (W/O) emulsion was created using 250 µL of water that was poured into the organic phase (DCM).…”

Section: Methodsmentioning

confidence: 99%

Combination of PDMS microfilters and micromixers based on flexible thermoplastic films for size sorting and mixing of microparticles

Lee

Lopez-Martinez

Baraket

et al. 2015

J of Applied Polymer Sci

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International audienceWe present a study for the development of flexible microfilters based on sealing microstructured poly(dimethylsiloxane) (PDMS) to different functionalized thermoplastic films [polyimide (PI), polyethylene naphthalate (PEN), and polyethylene terephthalate (PET)]. The microfilter was manufactured by soft-lithography and replica molding and then combined with plasma activation and chemical treatment using 3-(aminopropyl)triethoxysilane (APTES). To demonstrate the functionality of the PDMS microfilters, poly(lactic-co-glycolic acid) (PLGA) microparticles (MPS) were filtered through the microfluidic device based on the three thermoplastic films. Subsequently, the mixing capabilities of a passive PDMS micromixer was observed with the injection of polymeric MPS (fluorescent and nonfluorescent) as fluidic mixers are not generally effective at mixing particles. On mixing nonfluorescent MPS (approximate to<10-30 mu m in diameter) a mixing performance of 13.3% at 5 mm was observed. Therefore, a PDMS microfiltering device was integrated with a PDMS micromixer using a simple and cost effective home-made polymeric connector for filtration at a size sorting of 11 mu m. The results exhibit that the combination of the two microfluidic devices can be achieved with size sorting and mixing of MPS with an improved mixing performance of 62.5% at 3 mm

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“…The physico‐chemical double‐emulsion approach seems to be a suitable carrier to encapsulate FGF‐2 and TGF‐β1 growth factors into a biodegradable polymer of lactide and glycolide (PLGA). This physico‐chemical solvent extraction/evaporation technique is a widely used method to encapsulate hydrophilic drugs, especially proteins, peptides, and vaccines to ensure their long‐term release . It provides accurate control and reliable reproducibility of all physico‐chemical properties including the produced carrier microsphere size, morphology, loading and release profile, and hydrolysis in aqueous solutions .…”

Section: The Controlled Release Carriers Simulate the Local Microenvimentioning

confidence: 99%

Dentin–pulp regeneration: the primordial role of the microenvironment and its modification by traumatic injuries and bioactive materials

About¹

2013

Endodontic Topics

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Understanding dentinogenesis and pulp regeneration during physiological and pathological conditions represents a real challenge in the provision of a suitable treatment that ideally leads to the induction of the pulp regenerative potential. This paper focusses on the early steps of dentin–pulp regeneration that appear to be critical after pulp capping procedures. Different models are described in this paper where the interactions between different cell types in vitro illustrate their role in maintaining pulpal homeostasis. After traumatic injuries, the cells modify the local pulpal microenvironment by secreting growth factors that orchestrate and induce the processes required for dentin–pulp regeneration. Applying dental materials onto the injured pulp modifies this local microenvironment and affects the potential for pulpal regeneration. The paper also describes the added value of developing an entire human tooth culture model for understanding these early steps and discusses the interest of its use in evaluating newly developed pulp capping materials through the example of BiodentineTM, developed as a dentin substitute. The growth factors sustained release simulating the local microenvironment is also discussed. Simulating the pulp local environment with a continuous growth factors release is also a basic requirement for establishing future pulp tissue engineering.

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Colloidal and physicochemical characterization of proteincontaining poly(lactide-co-glycolide) (PLGA) microspheres before and after drying

Cited by 11 publications

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Progress in Nanoparticulate Systems for Peptide, Proteins and Nucleic Acid Drug Delivery

Progress in Nanoparticulate Systems for Peptide, Proteins and Nucleic Acid Drug Delivery

Combination of PDMS microfilters and micromixers based on flexible thermoplastic films for size sorting and mixing of microparticles

Dentin–pulp regeneration: the primordial role of the microenvironment and its modification by traumatic injuries and bioactive materials

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