Colloidal gold‐<scp>McAb</scp> probe‐based rapid immunoassay strip for simultaneous detection of fumonisins in maize

Yao, Jingjing; Sun, Yong; Li, Qingmei; Wang, Fangyu; Teng, Maikun; Yu, Yang; Deng, Ruiguang; Hu, Xiaofei

doi:10.1002/jsfa.8032

Cited by 27 publications

(15 citation statements)

References 20 publications

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“…Preparation of colloidal gold-mAb probe and ICS Colloidal gold was prepared as previously described with average particle diameter of approximately 24 nm [38]. The nanoparticles were conjugated with mAb as reported with slight modi cation [39].…”

Section: Competitive Elisamentioning

confidence: 99%

“…The strip consisted of a semirigid polyethylene sheet (backing card), a nitrocellulose (NC) membrane, a sample pad, a conjugate pad and an absorbent pad. [38] The NC membrane (20 mm × 30 mm) was spotted with one test line (T line) applied with CPH-BSA and one control line (C line) with goat anti-mouse IgG, with 0.5 cm between the two lines. The conjugate pad was treated with colloidal gold-mAb solution.…”

Section: Competitive Elisamentioning

confidence: 99%

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An Ultrasensitive Immunoassay Strip for Simultaneously Detecting Cyproheptadinehydrochloride and Six Phenothiazinesin Feedstuffs Based on a Monoclonal Antibody

Na¹,

Hu²,

Sun³

et al. 2020

Preprint

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Background: An antihistamine cyproheptadine (CPH) and phenothiazines (PZs)sedative hypnoticshave a similar tricyclic structure, these drugs are often illegally added to food animal feedstuffs due to their price advantage and significant effect in promoting growth and improving meat quality. However, the abuse of these drugs may lead totheir residues in animal products, thereby causing harmful effects such as allergies and dermatological reactions on human health.To supervise the use of prohibited drugs and ensure food safety, it is necessary to establish a simple and effective screening method to detect CPH and PZs.In this research, an artificial antigen against cyproheptadine(CPH) was successfully synthesized by bromoacetic acid substitution method. An ultrasensitive and broad-specific monoclonal antibody (mAb) able to recognize CPH and six phenothiazines (PZs) was produced. Based on the gold-labeled mAb, an immunochromatographic strip (ICS) was established.Results:The 50% inhibition concentration (IC50) of the produced mAb against CPH was identified as 0.036 ng mL-1 by ELISA, and the cross-reactivities for six PZs were from 6.33% to 63.16%. The visual detection limits(cut-off values) of the developed ICS ranged from 5 to 100ngg-1 in feedstuffs. Take a reading by strip reader, the IC50 was from 0.570 to 7.750ng g-1. In addition,a recovery experiment was carried out to verify the reliability of the ICS. The intra-assay recoveries were from 79.83% to 103.38% with the highest coefficient of variation (CV) of 11.31%. The inter-assay recoveries were from 79.00% to 96.63% with the highest CV of 12.66%.Conclusions: We have successfully produced a broad-spectrum monoclonal antibody and established an ICS for simultaneouslydetecting CPH and six PZs drugs.In brief, the proposed ICS was considered suitable for qualitatively and quantitatively monitoring CPH and PZs in feedstuffs.

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Section: Competitive Elisamentioning

confidence: 99%

Section: Competitive Elisamentioning

confidence: 99%

An Ultrasensitive Immunoassay Strip for Simultaneously Detecting Cyproheptadinehydrochloride and Six Phenothiazinesin Feedstuffs Based on a Monoclonal Antibody

Na¹,

Hu²,

Sun³

et al. 2020

Preprint

Self Cite

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“…Therefore it is essential to develop a rapid, simple and effective device for NIT detection in the field. Competitive format immunochromatographic strip assay based on the specific binding of antibody and antigen possesses distinct benefits for rapidly screening small analytes . Its user‐friendly format, simple analysis and low cost make it commonly used in real‐time detection of veterinary drug residues .…”

Section: Introductionmentioning

confidence: 99%

“…Competitive format immunochromatographic strip assay based on the specific binding of antibody and antigen possesses distinct benefits for rapidly screening small analytes. [19][20][21][22][23] Its user-friendly format, simple analysis and low cost make it commonly used in real-time detection of veterinary drug residues. [24][25][26][27][28] To the best of our knowledge, only one paper has reported an application for NIT detection based on the immunological method.…”

Section: Introductionmentioning

confidence: 99%

A rapid colloidal gold‐based immunochromatographic strip assay for monitoring nitroxynil in milk

Yang

et al. 2020

J Sci Food Agric

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BACKGROUND Nitroxynil (NIT) is a veterinary drug against hepatic fluke disease for food‐producing cattle and sheep. NIT has a long half‐life time in animals since it is highly bound to plasma protein. Therefore NIT possibly remains in animal edible tissues or milk due to drug abuse. In this study, a specific murine monoclonal antibody (mAb) against NIT was prepared and an immunochromatographic strip assay based on the mAb was developed for screening NIT in milk. RESULTS The affinity constant of the anti‐NIT mAb was 2.93 × 1010 and the anti‐NIT mAb had almost no cross‐reactivity with other analogs, so that it showed good specificity. The cutoff value of this test strip was considered to be 50 ng mL−1 by the naked eye. When detected by the strip reader, the half maximum inhibition concentration (IC50) of the immunoassay strip was calculated to be 5.716 ng mL−1 and the limit of detection (LOD) was 1.146 ng mL−1. Intra‐assay recoveries from 88.80 to 97.13% were obtained, with the highest coefficient of variation (CV) at 9.01%; inter‐assay recoveries ranged from 84.60 to 106.87%, with the highest CV at 9.93%. CONCLUSION The operative procedure of the proposed method can be completed within 10 min. The strip developed in this study was a practical tool for rapid semiquantitative and quantitative detection of NIT in milk. This study suggested great potential for analytically monitoring NIT in other food samples. © 2019 Society of Chemical Industry

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“…On the contrary, LFIST, which utilizes the specific interaction between antibody and antigen in a sandwich format 21 or competitive format 22 , is rapid and easy to be used by non-professionals. LFISTs for the specific detection of bacteria 23 , viruses 24 , pesticides 25 , veterinary drugs 26 – 28 , toxins 29 , 30 and allergens 31 , 32 have been developed and tested. However, there is no available LFIST for detecting OP currently.…”

Section: Introductionmentioning

confidence: 99%

A Lateral Flow Immunochromato-graphic Strip Test for Rapid Detection of Oseltamivir Phosphate in Egg and Chicken Meat

Yang

Wang

et al. 2018

Sci Rep

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A lateral flow immunochromatographic strip test (LFIST) based on a competitive format was developed for rapid and sensitive on-site detection of oseltamivir phosphate (OP) residues in poultry product. The sensitivity (half inhibitory concentration, IC50) of the LFIST in the detection of egg and chicken meat samples was confirmed to be 2.56 and 2.63 µg/kg, and the limit detection (LOD) value were 0.43 and 0.42 µg/kg, respectively. For intra-assay and inter-assay reproducibility, recoveries of OP spiked samples ranged between 82.8% and 91.2% with coefficients of variations (CV) less than 5.67% (intra-assay) and 6.52% (inter-assay). The performance of LFIST was comparable to high-performance liquid chromatography (HPLC) in a parallel testing of egg samples and chicken samples. LFIST takes less than 5 minutes, eliminates the dependency on professional personnel, and thus can be used as a surveillance tool for on-site detection of OP residues.

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Colloidal gold‐McAb probe‐based rapid immunoassay strip for simultaneous detection of fumonisins in maize

Abstract: The immunochromatographic test strip could be employed in the rapid simultaneous detection of FB , FB and FB in maize samples on-site. © 2016 Society of Chemical Industry.

Cited by 27 publications

References 20 publications

An Ultrasensitive Immunoassay Strip for Simultaneously Detecting Cyproheptadinehydrochloride and Six Phenothiazinesin Feedstuffs Based on a Monoclonal Antibody

An Ultrasensitive Immunoassay Strip for Simultaneously Detecting Cyproheptadinehydrochloride and Six Phenothiazinesin Feedstuffs Based on a Monoclonal Antibody

A rapid colloidal gold‐based immunochromatographic strip assay for monitoring nitroxynil in milk

A Lateral Flow Immunochromato-graphic Strip Test for Rapid Detection of Oseltamivir Phosphate in Egg and Chicken Meat

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