Colloidal Platinum in Hydrogen-Rich Water Exhibits Radical-Scavenging Activity and Improves Blood Fluidity

Abstract

The 'colloidal platinum' stabilized with polyvinylpyrrolidone (Pt/PVP-colloid) was dispersed in hydrogen-rich water (HW; hydrogen concentration, 0.82 ppm; oxidation-reduction potential, -583 mV) or regular water (RW; <0.01 ppm, +218 mV). And we evaluated the antioxidant activity of Pt/PVP-colloid in HW or RW on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and improvement of blood fluidity under 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress. When applied with the 0.25-0.5 ppm Pt/PVP-colloid in RW or HW, the level of DPPH radicals decreased to 77.5-59.6% or 16.1-5.6%, in contrast to the level as high as 81.3% for HW alone, respectively, as measured by an electron spin resonance method. The horse blood, which was subjected to AAPH-induced oxidative stress, was incubated for 24 hr with RW or HW, and thereafter required 13.7 sec (100%) or 5.7 sec (42.3%) for passing through the micro-channels in a rheology equipment. When treated with 0.5-1.0 ppm Pt/PVP-colloid in RW or HW, the blood passage time in the micro-channels decreased dose-dependently to 9.7-7.3 sec (71.6-53.8%) or 4.3-1.3 sec (32.8-10.3%), and the rate of micro-channels clogged with erythrocyte aggregates decreased to 23.8-21.0% or 15.8-9.8%, respectively, from 42.8% for no addition of Pt/PVP. By scanning electron microscopy, AAPH-treated erythrocytes lost intact surface morphology on the membrane together with protrusions and without hollows, being indicative of impaired transforming ability, and the rate of erythrocyte agglutination was increased to 46.2%. When treated the horse blood with HW alone significantly decreased the rate of erythrocyte agglutination to 29.6%, whereas 1.0 ppm Pt/PVP-colloid in RW or HW decreased it to 24.1% or 21.1%, respectively. Thus, DPPH-radical-scavenging and erythrocyte-protecting effects of Pt/PVP-colloid in HW were superior to those of Pt/PVP-colloid in RW or Pt/PVP-free HW. The results could be mainly attributed to the enhanced antioxidant activity of Pt/PVP in HW, which may be due to captured-hydrogen on platinum.

Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro

“…For comparing HW with RW, the water characteristic parameters were measured with the different dilution rates (Table 2, Figures 1 and 2). It must be emphasized that some stable characteristic indicators and the proprieties for innocuity and harmlessness of hydrogen water were obtained from several separated in vivo and human experiments which we had reported [19-23]. Meanwhile, via tube-feeding, PU patients were enforced to intake HW of 600 mL per day, in the morning and afternoon for approximately one hour, respectively, immediately after HW was manufactured everytime.…”

Electrolytically generated hydrogen warm water cleanses the keratin-plug-clogged hair-pores and promotes the capillary blood-streams, more markedly than normal warm water does

“…It was reported by our research team that rosary-like aggregation of erythrocytes was caused by oxidative stress, together with disappearance of the surface dent structure being necessary for passage of erythrocytes within the inside of a capillary vessel being more narrow than a diameter of erythrocytes, but prevented by hydrogen water, as observed by scanning electron microscopy. 14 As another mechanism that underlies the hydrogen-promoted blood streams, oxidative stress-induced delay of blood streams passing through narrow channels in a blood-streaming rheology comb-shaped apparatus was observed to be restored by hydrogen water. 14 …”

“… 14 As another mechanism that underlies the hydrogen-promoted blood streams, oxidative stress-induced delay of blood streams passing through narrow channels in a blood-streaming rheology comb-shaped apparatus was observed to be restored by hydrogen water. 14 …”

“…Although most drugs specifically act to their targets, a hydrogen molecule seems to differ from conventional pharmaceutical drugs, because a hydrogen molecule rapidly diffuses and militates moderately into tissues and cells. 14 By these reasons, we have been performed the application of nanomaterials with antioxidant properties on the anti-aging field because of their excellent activities to anti-inflammatory and healing effects 13 .…”

Electrospun Poly(ε-caprolactone) Composite Nanofibers with Controlled Release of Cis-Diamminediiodoplatinum for a Higher Anticancer Activity

“…Dozens of cisplatin analogs, such as carboplatin, oxaliplatin, nedaplatin, and lobaplatin, were synthesized and used in some limited range [4]. However, the efficacies of cisplatin and its analogs were primarily restricted by their poor water solubility, toxicity, and cross-resistance [5]. The rapid development of nanotechnology had promoted the in-depth study of platinum anticancer drugs [6].…”