Colloidal probe microscopy of membrane–membrane interactions: From ligand–receptor recognition to fusion events

Lorenz, Bärbel; Keller, Rabea; Sunnick, Eva; Geil, Burkhard; Janshoff, Andreas

doi:10.1016/j.bpc.2010.02.008

Cited by 23 publications

(32 citation statements)

References 44 publications

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“…for applications. Moreover, there is a large effort to develop models to recapitulate integral membrane proteins in supported membranes for controlled biophysical studies 26–28 . In order to study the interaction of membranes with transmembrane proteins, it is necessary to prevent deleterious interactions of the embedded protein with the underlying inorganic support.…”

Section: Introductionmentioning

confidence: 99%

Interaction Forces between DPPC Bilayers on Glass

Orozco-Alcaraz

Kuhl

2012

Langmuir

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The Surface Force Apparatus (SFA) was utilized to obtain force-distance profiles between silica supported membranes formed by Langmuir-Blodgett deposition of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). In the absence of a membrane, a long range electrostatic and short range steric repulsion is measured due to deprotonation of silica in water and roughness of the silica film. The electrostatic repulsion is partially screened by the lipid membrane and a van der Waals adhesion comparable to that measured with well packed DPPC membranes on mica is measured. This finding suggest that electrostatic interactions due to the underlying negatively charged silica are likely present in other systems of glass supported membranes. In contrast, the charge of an underlying mica substrate is almost completely screened when a lipid membrane is deposited on the mica. The difference in the two systems is attributed to stronger physisorption of zwitterionic lipids to molecularly smooth mica compared to rougher silica.

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Section: Introductionmentioning

confidence: 99%

Interaction Forces between DPPC Bilayers on Glass

Orozco-Alcaraz

Kuhl

2012

Langmuir

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“…Fluid bilayers display diffusion coefficients of (1.4 ± 0.1) μm 2 /s as determined by fluorescence recovery after photo-bleaching -only slightly reduced compared to giant liposomes or free standing bilayers. 15 Coupling of the epitopes was quantified by reflectometric interference spectroscopy (RIfS) revealing a coupling efficiency of 50 % for 2 . Details of the experimental procedures can be found in SI.…”

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confidence: 99%

Model System for Cell Adhesion Mediated by Weak Carbohydrate–Carbohydrate Interactions

Lorenz¹,

Cienfuegos

Oelkers³

et al. 2012

J. Am. Chem. Soc.

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The multivalent carbohydrate-carbohydrate interaction between membrane anchored epitopes derived from the marine sponge Microciona prolifera (M. prolifera) has been explored by colloidal probe microscopy. An in situ coupling of sulfated and non-sulfated disaccharides to membrane coated surfaces was employed to mimic native cell-cell contacts. The dynamic strength of the homomeric self-association was measured as a function of calcium ion concentration and loading rate. A deterministic model was used to estimate the number of participating bonds in the contact zone.

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“…Thus, we asked how the phosphorylation state of ezrin as well as PIP 2 binding alters the interaction forces as well as the adhesion energies of the ezrin/Factin interface. To probe the bond strength of ezrin/F-actin contacts, we made use of colloidal probe microscopy (23,24). As F-actin is a long filamentous protein (25) and ezrin forms protein clusters on membrane surfaces (26), it is conceivable that multiple ezrin/F-actin interactions occur in vivo and that the individual bond strength is weak.…”

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confidence: 99%