Colloidal stability of carboxylated iron oxide nanomagnets for biomedical use

Abstract

Magnetite nanoparticles (MNP)

Synthesis and characterization of superparamagnetic iron-oxide nanoparticles (SPIONs) and utilization of SPIONs in X-ray imaging

“…The increase in absorbance and change in pH in the further dilution was due to lack of monodispersion. It is already reported that the colloidal stability is greatly influenced by pH and which is contributed by organic acids (Tombácz et al 2013(Tombácz et al , 2014. The reduction in pH value could be attributed by the disappearance of organic molecular hydrogen from the solution into the SPIONs as the interstitial element which is further substantiated with NMR results.…”

Hemocompatibility and Biomedical Potential of Poly(Gallic Acid) Coated Iron Oxide Nanoparticles for Theranostic Use

“…TEM pictures of naked and PGA-coated MNPs are given in Seciton 1 of Supplementary material (Figures S1 and S2) demonstrating also the formation of a thick PGA coating. Particles with not fully polymerized GA shell (GA@MNPs) were also characterized earlier [21,22]. Although they were found to be highly biocompatible (cell viability inhibition < 5%), their low colloidal stability made them inapplicable for biomedical application.…”

“…The pH of magnetite dispersions was adjusted to ∼6.5 and GA was added in concentration of 0.6 mmol GA/g magnetite. The pH was readjusted subsequently for five weeks, during which period GA polymerized at the surface as it is demonstrated in [21,22]. The sample was stored in dark at 4 o C. The values of electrokinetic potential (-40 mV), hydrodynamic diameter (∼170 nm, as compared to that of naked MNPs of ∼100 nm) and critical coagulation concentration (500 mM, while that of naked MNPs of is only 1 mM) revealed the presence of a thick coating layer, which in turn, stabilizes the particles even in the presence of salt at high concentrations.…”

“…The PGA@MNPs for the biomedical tests were prepared on the basis of GA adsorption experiments detailed by us earlier [21,22]. The PGA@MNP dispersions were prepared at a concentration of 15 g/L in aqueous medium.…”

“…This way, the synthesis of PGA@MNPs would proceed in an all-natural soft method and the emergence of toxic by-products would be excluded. The PGA@MNPs have indeed been successfully produced and we have published their physico-chemical and colloidal characterization relevant to possible biomedical applications [20][21][22]. In the present study, we utilized in vitro experiments for testing the biodistribution, biocompatibility and antioxidant capacity of the polygallate stabilized SPION product.…”